LUCAS RIBEIRO XAVIER CORTELLA
Índice h a partir de 2011
2
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/65, Hospital das Clínicas, Faculdade de Medicina
LIM/65, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
Agora exibindo 1 - 3 de 3
- Surface Topography Obtained with High Throughput Technology for hiPSC-Derived Cardiomyocyte Conditioning(2022) CORTELLA, Lucas R. X.; CESTARI, I. A.; S, M.; MAZZETTO, M.; LASAGNI, A. F.; CESTARI, Ismar N.The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) to replace myocardial tissue after an infarct holds great promises. However, hiPSC-CM are phenotypically immature when compared to cells in the adult heart, hampering their clinical application. We aimed to develop and test a surface structuring technique that would improve hiPSC-CM structural maturation. Laser ablation was used to fabricate a micron-pattern on polyurethane surface and evaluated cell morphology, orientation and F-actin assemblage to detect phenotypic changes in response to the microtopography. This topography positively influenced cell morphology regarding to spreading area and elongation, and hiPSC-CM orientation, improving their structural maturation. The methodology thus presented has relatively low cost and is easily scalable, making it relevant for high-throughput applications such as drug screening for the pharma industry.
- Blood cell adhesion to arterial filters analysis by scanning electron microscopy and real-time PCR assay: observational clinical study in cardiac surgery patients(2022) GATTO, Chiara Scaglioni Tessmer; PICCIONI, Marilde Albuquerque; STRUNZ, Celia Maria Cassaro; CESTARI, Idagene Aparecida; CUNHA, Ligia Cristina Camara; ROGGERIO, Alessandra; SILVA, Vanessa Monteiro da; ZUCCATO, Maria Cecilia Freire; CORTELLA, Lucas Ribeiro Xavier; KAKOI, Adelia Aparecida Yuka; JATENE, Fabio Biscegli; COSTA JUNIOR, Jose Otavio Auler da; GALAS, Filomena Regina Barbosa GomesIntroduction: Arterial filter is the part of the cardiopulmonary bypass circuit where blood cells are exposed to high mechanical stress and where cellular aggregates may fasten in large quantities. The aim of this study was to analyse blood cell adhesiveness in the arterial filter through scanning electron microscopy and real-time PCR assay. Methods: Prospective, clinical and observational study performed on 28 patients undergoing cardiac surgery with cardiopulmonary bypass. Arterial filters were analysed by scanning electron microscopy. Real-time PCR assay was performed in extracted material from the arterial filters for analysis of platelet GPIb and CD45 leucocyte gene expression. Blood coagulation was analysed during cardiopulmonary bypass. Patients were followed until hospital discharge or 28 days after surgery. Results: All studied arterial filters used in the subject patients showed a degree of adhesion from blood elements at scanning electron microscopy. All studied filters were positive for platelets GPIb gene expression and 15% had CD45 leucocyte gene expression. The GPIb platelet gene expression in blood lowered at the end of cardiopulmonary bypass (p = 0.019). There was negative correlation between blood GPIb platelet gene expression and Clot SR (HEPSCREEN2 ReoRox(R)) (rho = 0.635; p = 0.027). The filter fields count was correlated to the D-dimer dosage (rho = 0.828; p < 0.001). Conclusion: There was adhesion of blood elements, especially nucleated platelets, on all arterial filters studied. Although the arterial filter worked as a safety device, that possibly prevented arterial embolisation, it may also have caused greater hyperfibrinolysis during cardiopulmonary bypass.
- Experimental Apparatus for Evaluation of Calcium Fluctuations in Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells(2022) ARANA, M. C.; LAHUERTA, R. D.; CORTELLA, L. R. X.; MAZZETTO, M.; SOLDERA, M.; LASAGNI, A. F.; CESTARI, I. N.; CESTARI, I. A.In this work, we developed and tested an experimental apparatus to evaluate calcium fluctuations in cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM). The set-up is composed of a signal module for registration and analysis of the signals and a perfusion chamber. This chamber allows the maintenance of the cells, control of perfusion and temperature, and electric stimulation. The signal module consists of a CCD camera attached to a fluorescence microscope with the appropriate software and hardware for eliciting and recording fluorescent signals originating from hiPSC-CM intracellular Ca+2 concentration changes, under electrical stimulation. We employed this system for analysis of calcium fluctuation from hiPSC-CM cultivated on micro textured polyethylene terephthalate surfaces.