LUCAS RIBEIRO XAVIER CORTELLA
Índice h a partir de 2011
2
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/65, Hospital das Clínicas, Faculdade de Medicina
LIM/65, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
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- Blood cell adhesion to arterial filters analysis by scanning electron microscopy and real-time PCR assay: observational clinical study in cardiac surgery patients(2022) GATTO, Chiara Scaglioni Tessmer; PICCIONI, Marilde Albuquerque; STRUNZ, Celia Maria Cassaro; CESTARI, Idagene Aparecida; CUNHA, Ligia Cristina Camara; ROGGERIO, Alessandra; SILVA, Vanessa Monteiro da; ZUCCATO, Maria Cecilia Freire; CORTELLA, Lucas Ribeiro Xavier; KAKOI, Adelia Aparecida Yuka; JATENE, Fabio Biscegli; COSTA JUNIOR, Jose Otavio Auler da; GALAS, Filomena Regina Barbosa GomesIntroduction: Arterial filter is the part of the cardiopulmonary bypass circuit where blood cells are exposed to high mechanical stress and where cellular aggregates may fasten in large quantities. The aim of this study was to analyse blood cell adhesiveness in the arterial filter through scanning electron microscopy and real-time PCR assay. Methods: Prospective, clinical and observational study performed on 28 patients undergoing cardiac surgery with cardiopulmonary bypass. Arterial filters were analysed by scanning electron microscopy. Real-time PCR assay was performed in extracted material from the arterial filters for analysis of platelet GPIb and CD45 leucocyte gene expression. Blood coagulation was analysed during cardiopulmonary bypass. Patients were followed until hospital discharge or 28 days after surgery. Results: All studied arterial filters used in the subject patients showed a degree of adhesion from blood elements at scanning electron microscopy. All studied filters were positive for platelets GPIb gene expression and 15% had CD45 leucocyte gene expression. The GPIb platelet gene expression in blood lowered at the end of cardiopulmonary bypass (p = 0.019). There was negative correlation between blood GPIb platelet gene expression and Clot SR (HEPSCREEN2 ReoRox(R)) (rho = 0.635; p = 0.027). The filter fields count was correlated to the D-dimer dosage (rho = 0.828; p < 0.001). Conclusion: There was adhesion of blood elements, especially nucleated platelets, on all arterial filters studied. Although the arterial filter worked as a safety device, that possibly prevented arterial embolisation, it may also have caused greater hyperfibrinolysis during cardiopulmonary bypass.
- Experimental Method for Recording Epicardium Potentials and Cardiac Myocyte Shortening(2017) MARCHINI, Gustavo S.; TAMASHIRO, Daniel S. U.; OYAMA, Helena T.; CORTELLA, Lucas; CESTARI, Ismar N.; CESTARI, Idagene A.The hemodynamic changes observed in advanced stages of heart diseases are often accompanied by changes in the electrical and mechanical properties of cardiac myocytes. The objective of this work is to develop an experimental method for recording ventricle epicardium potentials in isolated rat hearts and isolated cardiac myocyte shortening. Briefly, rat heart was removed, aorta was cannulated and coronary arteries were retrogradely perfused with heated and oxygenated buffer solutions. Ag-AgCl electrodes fixed in a silicone pouch placed around the heart were used to measure epicardium potentials. The perfusion was switched to an enzyme-containing solution for digestion of the heart and obtaining isolated cardiac myocytes. Measurements of shortening were made in cells electrically stimulated. The results suggest the possibility of relating the electrical behavior of the whole heart with mechanical properties of cardiac myocytes and may represent an useful tool in basic cardiac research.