LUCAS RIBEIRO XAVIER CORTELLA
Índice h a partir de 2011
2
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/65, Hospital das Clínicas, Faculdade de Medicina
LIM/65, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- Surface Topography Obtained with High Throughput Technology for hiPSC-Derived Cardiomyocyte Conditioning(2022) CORTELLA, Lucas R. X.; CESTARI, I. A.; S, M.; MAZZETTO, M.; LASAGNI, A. F.; CESTARI, Ismar N.The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) to replace myocardial tissue after an infarct holds great promises. However, hiPSC-CM are phenotypically immature when compared to cells in the adult heart, hampering their clinical application. We aimed to develop and test a surface structuring technique that would improve hiPSC-CM structural maturation. Laser ablation was used to fabricate a micron-pattern on polyurethane surface and evaluated cell morphology, orientation and F-actin assemblage to detect phenotypic changes in response to the microtopography. This topography positively influenced cell morphology regarding to spreading area and elongation, and hiPSC-CM orientation, improving their structural maturation. The methodology thus presented has relatively low cost and is easily scalable, making it relevant for high-throughput applications such as drug screening for the pharma industry.
- Conditioning of hiPSC-derived cardiomyocytes using surface topography obtained with high throughput technology(2021) CORTELLA, Lucas R. X.; CESTARI, Idagene A.; LAHUERTA, Ricardo D.; ARANA, Matheus C.; SOLDERA, Marcos; RANK, Andreas; LASAGNI, Andres F.; CESTARI, Ismar N.Surface functionalization of polymers aims to introduce novel properties that favor bioactive responses. We have investigated the possibility of surface functionalization of polyethylene terephthalate (PET) sheets by the combination of laser ablation with hot embossing and the application of such techniques in the field of stem cell research. We investigated the response of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) to topography in the low micrometer range. HiPSC-CMs are expected to offer new therapeutic tools for myocardial replacement or regeneration after an infarct or other causes of cardiac tissue loss. However, hiPSC-CMs are phenotypically immature compared to myocytes in the adult myocardium, hampering their clinical application. We aimed to develop and test a high-throughput technique for surface structuring that would improve hiPSC-CMs structural maturation. We used laser ablation with a ps-laser source in combination with nanoimprint lithography to fabricate large areas of homogeneous micron- to submicron line-like pattern with a spatial period of 3 mu m on the PET surface. We evaluated cell morphology, alignment, sarcomeric myofibrils assembly, and calcium transients to evaluate phenotypic changes associated with culturing hiPSC-CMs on functionalized PET. Surface functionalization through hot embossing was able to generate, at low cost, low micrometer features on the PET surface that influenced the hiPSC-CMs phenotype, suggesting improved structural and functional maturation. This technique may be relevant for high-throughput technologies that require conditioning of hiPSC-CMs and may be useful for the production of these cells for drug screening and disease modeling applications with lower costs.
- Experimental Apparatus for Evaluation of Calcium Fluctuations in Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells(2022) ARANA, M. C.; LAHUERTA, R. D.; CORTELLA, L. R. X.; MAZZETTO, M.; SOLDERA, M.; LASAGNI, A. F.; CESTARI, I. N.; CESTARI, I. A.In this work, we developed and tested an experimental apparatus to evaluate calcium fluctuations in cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM). The set-up is composed of a signal module for registration and analysis of the signals and a perfusion chamber. This chamber allows the maintenance of the cells, control of perfusion and temperature, and electric stimulation. The signal module consists of a CCD camera attached to a fluorescence microscope with the appropriate software and hardware for eliciting and recording fluorescent signals originating from hiPSC-CM intracellular Ca+2 concentration changes, under electrical stimulation. We employed this system for analysis of calcium fluctuation from hiPSC-CM cultivated on micro textured polyethylene terephthalate surfaces.
- Endothelial cell responses to castor oil-based polyurethane substrates functionalized by direct laser ablation(2017) CORTELLA, L. R. X.; CESTARI, I. A.; GUENTHER, D.; LASAGNI, A. F.; CESTARI, I. N.Surface-induced thrombosis and lack of endothelialization are major drawbacks that hamper the widespread application of polyurethanes for the fabrication of implantable cardiovascular devices. Endothelialization of the blood-contacting surfaces of these devices may avoid thrombus formation and may be implemented by strategies that introduce micro and submicron patterns that favor adhesion and growth of endothelial cells. In this study, we used laser radiation to directly introduce topographical patterns in the low micrometer range on castor oil-based polyurethane, which is currently employed to fabricate cardiovascular devices. We have investigated cell adhesion, proliferation, morphology and alignment in response to these topographies. Reported results show that line-like and pillar-like patterns improved adhesion and proliferation rate of cultured endothelial cells. The line-like pattern with 1 mu m groove periodicity was the most efficient to enhance cell adhesion and induced marked polarization and alignment. Our study suggests the viability of using laser radiation to functionalize PU-based implants by the introduction of specific microtopography to facilitate the development of a functional endothelium on target surfaces.
- Experimental Method for Recording Epicardium Potentials and Cardiac Myocyte Shortening(2017) MARCHINI, Gustavo S.; TAMASHIRO, Daniel S. U.; OYAMA, Helena T.; CORTELLA, Lucas; CESTARI, Ismar N.; CESTARI, Idagene A.The hemodynamic changes observed in advanced stages of heart diseases are often accompanied by changes in the electrical and mechanical properties of cardiac myocytes. The objective of this work is to develop an experimental method for recording ventricle epicardium potentials in isolated rat hearts and isolated cardiac myocyte shortening. Briefly, rat heart was removed, aorta was cannulated and coronary arteries were retrogradely perfused with heated and oxygenated buffer solutions. Ag-AgCl electrodes fixed in a silicone pouch placed around the heart were used to measure epicardium potentials. The perfusion was switched to an enzyme-containing solution for digestion of the heart and obtaining isolated cardiac myocytes. Measurements of shortening were made in cells electrically stimulated. The results suggest the possibility of relating the electrical behavior of the whole heart with mechanical properties of cardiac myocytes and may represent an useful tool in basic cardiac research.