RICARDO DOS SANTOS SIMOES
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
SCGINEC-62, Hospital Universitário
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
39 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 39
- Isoflavones in gynecology(2018) CARBONEL, Adriana Aparecida Ferraz; SIMOES, Ricardo Santos; GIRAO, Joao Henrique Castello; SASSO, Gisela Rodrigues da Silva; BERTONCINI, Clelia Rejane Antonio; SORPRESO, Isabel Cristina Esposito; SOARES JUNIOR, Jose Maria; SIMOES, Manuel Jesus; BARACAT, Edmund Chada
- Glycosaminoglycan profiles in the uterus of adult ovariectomized rats treated with estrogen and progestagen(2012) SIMOES, Ricardo Santos; OLIVEIRA-FILHO, Ricardo Martins; NADER, Helena Bonciani; BARACAT, Edmund ChadaObjectives: To evaluate the effects of conjugated equine estrogens (CE) alone or in combination with medroxyprogesterone acetate (MPA) on glycosaminoglycans (GAGs) in the cervix and horns of the rat uterus. Study design: Thirty days after ovariectomy, adult rats were randomly divided into four groups: Cl, control (treated with drug vehicle); GII CE (50 mu g/kg per day); GIII, MPA (0.2 mg/kg per day), and GIV, CE + MPA (doses as in GII and Gill). Drugs and vehicle were given by gavage during 28 days. Afterwards the animals were anesthetized, the cervix and uterine horns were dissected out and the middle portion fixed in 10% formaldehyde solution; other portions were fixed in acetone for histological examination and glycosaminoglycan quantification, respectively. Agarose gel electrophoresis was used for sulfated GAG analyses, and hyaluronic acid was assayed with an ELISA-like method. Statistical analysis was done by the Student's t test and the Tukey-Kramer test (P < 0.05). Results: The cervix and uterine horn structures presented signs of atrophy in the control group (GI). The other groups, mainly groups III and IV, had histological aspects of proliferation. In all groups the concentration of sulfated GAGs (especially dermatan sulfate) was higher than that of non-sulfated GAGs, both in cervix and in uterine horns. Estrogens increased sulfated GAG concentration at the cervix and the horn, whereas in uterine horns the amounts of sulfated GAGs were decreased after estrogens plus MPA treatment. The concentration of hyaluronic acid in uterine horns was higher than in cervices. Conclusions: The profiling and amounts of glycosaminoglycans in the two portions of the rat uterus are uneven. Dermatan sulfate occurs in higher concentrations in both cervix and uterine horns. Sulfated GAGs in rat cervix were increased by estrogens plus MPA, but were decreased by MPA alone in uterine horns.
- The effects of soybean isoflavones and 17-estradiol in uterus and mammary glands of diabetic rat models(2018) CARBONEL, Adriana Aparecida Ferraz; LIMA, Patricia Daniele Azevedo; LIM, Jung Jin; FUCHS, Luiz Fernando Portugal; PAIOTTI, Ana Paula Ribeiro; SASSO, Gisela Rodrigues da Silva; SIMOES, Ricardo Santos; SOARES JUNIOR, Jose Maria; BARACAT, Edmund Chada; SIMOES, Manuel de JesusThe objective of this study was to evaluate the action of soy isoflavones and 17 beta estradiol on the extracellular matrix in the uterus and mammary gland of diabetic rats. Sixty adult female rats underwent ovariectomy, then randomized into seven groups of ten animals each: Non-diabetic: GI Sham control animals ovariectomized; and GII control ovariectomized that received propylene glycol vehicle. Diabetic: GIII Sham control diabetic animals ovariectomized; GIV ovariectomized diabetic animals receiving propylene glycol vehicle; GV diabetic ovariectomized animals treated with soy isoflavones (150mg/kg by gavage); GVI ovariectomized diabetic rats treated with estrogen (17b-estradiol, 10mg/kg, subcutaneously); GVII diabetic ovariectomized animals treated with soy isoflavones (150 mg/kg by gavage), and with estrogen (17b-estradiol, 10 mg/kg combination therapy). Treatments occurred during 30 consecutive days. After animals euthanasia, a portion of the uterus was immersed in liquid nitrogen for molecular biology analysis, the other portion of uterus and mammary glands were removed and processed for paraffin embedding. Soy isoflavones (GV) and 17b estradiol improved the production of compounds of extracellular matrix, such as small leucine-rich proteoglycans (SLRPs). The combination of both therapies had an additive effect in SLRPs expression. Soy isoflavones contribute to the uterine integrity of SLRPs of diabetic rats.
conferenceObject Elevation of collagen and proteoglycan in diabetic rat vaginas treated with soybean isoflavones, 17 beta-estradiol and combined therapy(2016) CARBONEL, Adriana A.; SIMOES, Ricardo Santos; BERTONCINI, Clelia R.; SOARES JR., Jose M.; SIMOES, Manuel J.- Evaluation of the isoflavones and estrogen effects on the rat adrenal(2017) MARINHO, Darci Souza; CALIO, Michele Longoni; SANTOS, Miriam Aparecida; KO, Gui Mi; TEIXEIRA, Cristiane Paula; CARBONEL, Adriana Ferraz; LISBOA-NASCIMENTO, Telma; CASTRO, Rodrigo Aquino; SIMOES, Ricardo Santos; BERTONCINI, Clelia Rejane AntonioThe aim of this study was to evaluate the morphometry and the gene expression of Ki-67, VEGF and caspase 3 and the stress oxidative in the adrenal gland of ovariectomized rats treated with estrogen or isoflavones. We used 15 Wistar rats ovariectomized treated with isoflavones or estrogen during 30days. At the end of the treatment, the left adrenal gland was removed for subsequent histological studies and the right was used to evaluate gene expression of angiogenesis (VEGF-A), cell proliferation (Ki-67), apoptose (caspase 3 clivated) and oxidative stress. Treatment with estrogen showed a largest increase in the layers of the adrenal cortex than with isoflavones. These hypertrofic effects agree with higher expression elevation of Ki67 and VEGF, which did not occur with the caspase 3, indicating that isoflavones have great proliferative effect on the adrenal gland. Similar results were also observed on superoxide quantification show that isoflavone has a protective effect against oxidative stress. Our results indicate positively the trophic therapeutic potential of isoflavones has a protective effect and can contribute to the development of effective therapies to decrease the symptoms of menopause.
- Endométrio na janela de implantação em mulheres com síndrome dos ovários policísticos(2011) LOPES, Ione Maria Ribeiro Soares; BARACAT, Maria Candida Pinheiro; SIMOES, Manuel de Jesus; SIMOES, Ricardo Santos; BARACAT, Edmund Chada; SOARES JR., Jose MariaEndometrium in women with polycystic ovary syndrome during the window of implantation The human endometrium undergoes to a complex series of proliferative and secretory changes in each menstrual cycle and displays only a short period of receptivity, known as the ""window of implantation"", necessary for the implantation of the blastocyst in the uterus. The implantation process occurs in a sequential manner, leading to the establishment of pregnancy. Morphofunctional changes during this period may prevent or hinder the implantation. For this reason, the study of the endometrium at this stage is important for the improvement of therapies that may interfere with the mechanisms involved in maternal-embryonic interaction. Several gynecological disorders, including polycystic ovary syndrome (PCOS), are associated with decreased fertility and uterine receptivity. In spite of recent advances in assisted reproduction techniques, allowing the selection of high quality embryos, the implantation rate remains low and has not increased enough in recent decades. This article aims at reviewing the endometrial aspects of the ""window of implantation"" in women with polycystic ovary syndrome, focusing mainly on adhesion molecules. For that purpose, we analyzed 105 articles published in journals indexed in PubMed in the last 50 years (up to May 2011). In conclusion, the endometrial receptivity seems to be the major limiting factor for the establishment of pregnancy in a large number of gynecological diseases, including PCOS, and treatment to improve implantation rates is likely to be taken towards this direction.
- Imbalance between the cellular proliferation and death in harderian gland of female mice with hyperprolactinemia(2021) ARAUJO, Ariadne Stavare Leal; VERNA, Carina; ARAUJO JUNIOR, Osvaldo Pereira; SOARES JUNIOR, Jose Maria; SIMOES, Ricardo Santos; BARACAT, Edmund Chada; SIMOES, Manuel de Jesus; GOMES, Regina Celia TeixeiraOBJECTIVE: To assess the impact of the metoclopramide-induced hyperprolactinemia in cellular death and proliferation in the harderian gland of female mice. METHODS: Twenty female mice were divided into two groups of 10 animals each and treated: 0.2 mL of saline solution (controls, Ctr) and 200 mu g of metoclopramide (experimental, hyperprolactinemia), both for 50 consecutive days and at 12:00 a.m. On the 50th day, the female were euthanized, and the harderian glands were removed and processed for immunohistochemistry for detected ki67 and TUNEL method. Data were statistically analyzed by unpaired Student's t test (p<0.05). RESULTS: The harderian gland of the hyperprolactinemia group showed increase in the immunoexpression of Ki67 and TUNEL compared to the Ctr group (p<0.05), and there was no significant difference in the amount of porphyrin in the HPrl group compared to the Ctr group. CONCLUSION: The hyperprolactinemia led to increased cell death in the acini the harderian gland and cell proliferation in the stroma glandular, fact that suggesting a reduction process of cellular activity and fibrosis, which suggests impairment in the functioning of the lacrimal harderian.
- Histomorphometric and immunohistochemical changes in interstitial cells and ovarian follicles of rats with polycystic ovaries treated with clomiphene citrate(2021) LOMBARDI, Leonardo Augusto; MATTOS, Leandro Sabara; SIMOES, Ricardo Santos; FLORENCIO-SILVA, Rinaldo; SIMOES, Manuel de Jesus; FRANCO, Paulo Celso; CARBONELL, Adriana Aparecida Ferraz; SASSO, Gisela Rodrigues da Silva; BARACAT, Edmund Chada; SOARES-JR, Jose MariaObjective To evaluate the histomorphometric and immunohistochemical changes in interstitial cells and ovarian follicles of rats treated with clomiphene citrate during and after induction of permanent estrus. Methods Twenty four adult-female rats with regular estrous cycle were equally divided into three groups: (1) GCtrl-at estrous phase. (2) GPCOS-at permanent-estrous phase. (3) GCC-PCOS rats, which remained exposed to 60 days of continuous illumination and treated with Clomiphene Citrate. After that, the animals were euthanized, and the ovaries were removed and processed for paraffin embedding. Sections were stained with H.E. for histomorphometry or subjected to immunohistochemistry for Ki-67 and cleaved caspase-3 detections. Results The GPCOS showed lack of corpus luteum and several ovarian cysts, as well as interstitial-like cells. The presence of corpus luteum and a significant increase in primary and antral follicles were observed in GCC, which also showed a decrease in the number of ovarian cysts and in the area occupied by interstitial-like cells, as well as a decrease in nuclear volume of interstitial cells. The percentage of cell proliferation was significantly higher in granulosa cells of the GCC. On the other hand, the percentage of apoptosis was significantly higher in the granulosa cells of GPCOS than the GCC. Conclusion The ovaries of rats treated with clomiphene citrate showed a decrease in the number of cysts, an increase in the number of ovarian follicles, the presence of corpus luteum along with a decrease in the nuclear volume in the area occupied by interstitial cells.
- May isoflavones prevent breast cancer risk?(2022) CARBONEL, Adriana Aparecida Ferraz; SIMOES, Ricardo Santos; SASSO, Gisela da Silva; VIEIRA, Renata Ramos; LIMA, Patricia Azevedo; SIMOES, Manuel de Jesus; JR, Jose Maria Soares
- Estrogens: possible protection against Amyotrophic Lateral Sclerosis?(2019) VASCONCELOS, Katia de; OLIVEIRA, Acary Souza Bulle; FUCHS, Luiz Fernando Portugal; SIMOES, Ricardo Santos; GIRAO, Manoel Joao Batista Castello; SOARES JUNIOR, Jose Maria; BARACAT, Edmund Chada