MARCIO CORREA MANCINI
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/18 - Laboratório de Carboidratos e Radioimunoensaios, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/18 - Laboratório de Carboidratos e Radioimunoensaios, Hospital das Clínicas, Faculdade de Medicina - Líder
11 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 11
- Lack of mutations in the leptin receptor gene in severely obese children(2012) DIAS, Natasha Favoretto; FERNANDES, Ariana Ester; MELO, Maria Edna de; REINHARDT, Heidi Lui; CERCATO, Cintia; VILLARES, Sandra Mara Ferreira; HALPERN, Alfredo; MANCINI, Marcio C.Objective: To analyze the LEPR gene in obese children and to investigate the associations between molecular findings and anthropometric and metabolic features. Subjects and methods: Thirty-two patients were evaluated regarding anthropometric characteristics, blood pressure, heart rate, serum glucose, insulin, leptin levels, and lipid profile. The molecular study consisted of the amplification and automatic sequencing of the coding region of LEPR in order to investigate new mutations. Results: We identified a high prevalence of metabolic disorders: impaired fasting glucose in 12.5% of the patients, elevated HOMA-IR in 85.7%, low HDL-cholesterol levels in 46.9%, high triglyceride levels in 40.6%, and hypertension in 58.6% of the patients. The molecular study identified 6 already described allelic variants: rs1137100 (exon-2), rs1137101 (exon-4), rs1805134 (exon-7), rs8179183 (exon-12), rs1805096 (exon-18), and the deletion/insertion of the pentanucleotide CTTTA at 3'untranslated region. Conclusions: The frequency of alleles observed in this cohort is similar to that described in the literature, and was not correlated with any clinical feature. The molecular findings in the analysis of the LEPR did not seem to be implicated in the etiology of obesity in these patients.
- Differences in the gut microbiota of women according to ultra- processed food consumption(2023) FERNANDES, Ariana E.; ROSA, Paula W. L.; MELO, Maria E.; MARTINS, Roberta C. R.; SANTIN, Fernanda G. O.; MOURA, Aline M. S. H.; COELHO, Graziele S. M. A.; SABINO, Ester C.; CERCATO, Cintia; MANCINI, Marcio C.Background and aims: High consumption of ultra-processed food (UPF) has been associated with increased risk of obesity and other metabolic diseases, and this dietary pattern seems to be responsible for chronic changes in the gut microbiota. The aim of this study was to assess the associations of UPF with the gut microbiota and obesity-associated biometrics in women. Methods and results: This cross-sectional study examined 59 women. The following parameters were evaluated: food consumption using NOVA classification, anthropometric and metabolic parameters, and gut microbiome by next-generation sequencing. The mean age was 28.0 & PLUSMN; 6.6 years. The mean caloric intake was 1624 & PLUSMN; 531 kcal, of which unprocessed or minimally processed food (G1) accounted for 52.4 & PLUSMN; 13.5%, and UPF accounted for 31.4 & PLUSMN; 13.6%. Leptin levels adjusted for fat mass were negatively associated with G1 and positively associated with UPF. We found 15 species in the gut microbiota that correlated with G1 (3 positively and 12 negatively) and 9 species associated with UPF (5 positively and 4 negatively). Conclusion: Higher consumption of UPF was directly associated with leptin resistance, and this study suggests that the consumption of UPF or G1 may affect the composition of the gut micro biota. & COPY; 2022 The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University.
- Effects of two diet techniques and delivery mode on weight loss, metabolic profile and food intake of obese adolescents: a fixed diet plan and a calorie-counting diet(2017) MENDES, M. D. S. D.; MELO, M. E. de; FERNANDES, A. E.; FUJIWARA, C. T. H.; PIOLTINE, M. B.; TEIXEIRA, A.; COELHO, K.; GALASSO, M.; CERCATO, C.; MANCINI, M. C.The aim of this study is to compare the weight loss of obese adolescents on two different low-calorie diets: fixed diet plan and calorie-counting diet. This is a randomized clinical study with 66 obese adolescents (body mass index Z score (ZBMI) > + 3, 13.7 +/- 0.7 years, 60.6% male) with anthropometric, food intake, physical activity, laboratory, body composition and stage of pubertal development data evaluated. There was a reduction in the ZBMI in both groups (P < 0.0001), without significant difference between them (P = 0.87). There was a significant reduction in insulin, and homeostasis model assessment insulin resistance (HOMA-IR), with no difference between groups. A reduction in total energy intake of the groups was found, with an increase in the proportion of protein and reduction in carbohydrates. In this cohort of severely obese adolescents, fixed diet plan and calorie-counting diet led to a similar reduction of ZBMI, metabolic markers and total energy intake.
conferenceObject ASSOCIATION BETWEEN RS1137100 SNP IN LEPR GENE AND WAIST-TO-HEIGHT RATIO IN BRAZILIAN OVERWEIGHT CHILDREN AND ADOLESCENTS(2013) FUJIWARA, C. T. H.; FERNADES, A. E.; MELO, M. E.; SANTOS, A.; CERCATO, C.; HALPERN, A.; MANCINI, M. C.conferenceObject RS1137101 SNP IN LEPR GENE IS NOT ASSOCIATED WITH BINGE EATING AND CARDIOMETABOLIC RISK IN BRAZILIAN OVERWEIGHT CHILDREN AND ADOLESCENTS(2013) FUJIWARA, C. T. H.; FERNANDES, A. E.; MELO, M. E.; SANTOS, A.; CERCATO, C.; HALPERN, A.; MANCINI, M. C.conferenceObject RS12970134 IN MC4R GENE IS NOT RELATED TO WORST METABOLIC PROFILE IN OVERWEIGHT CHILDREN(2013) FERNANDES, A. E.; FUJIWARA, C. T. H.; MELO, M. E.; SANTOS, A.; CERCATO, C.; HALPERN, A.; MANCINI, M.- Associations between a common variant near the MC4R gene and serum triglyceride levels in an obese pediatric cohort(2015) FERNANDES, Ariana Ester; MELO, Maria Edna de; FUJIWARA, Clarissa Tamie Hiwatashi; PIOLTINE, Marina Brosso; MATIOLI, Sergio Russo; SANTOS, Aritania; CERCATO, Cintia; HALPERN, Alfredo; MANCINI, Marcio C.Polymorphisms near the MC4R gene may be related to an increased risk for obesity, but studies of variations in this gene and its relation to cardiometabolic profiles and food intake are scarce and controversial. The aim of this study is to evaluate the influence of the variants rs12970134 and rs17782313 near the MC4R gene in food intake, binge eating (BE) behavior, anthropometric parameters, body composition, metabolic profile, and cardiometabolic risk factors in obese children and adolescents. This is a cross-sectional study that included obese children and adolescents. We evaluated anthropometric, metabolic parameters and cardiometabolic risk factors, including hypertension, impaired fasting glucose, hypertriglyceridemia, and low HDL-cholesterol. BE was assessed through the BE scale, and a 24-h recall was used to evaluate total caloric intake and percentage of macronutrients and types of dietary fat. The MC4R variants rs12970134 and rs17782313 were genotyped using TaqMan assay. To assess the magnitude of risk, a logistic regression adjusted for Z-BMI, age, and gender was performed, adopting the significance level of 0.05. The study included 518 subjects (52.1 % girls, 12.7 +/- A 2.7 years old, Z-BMI = 3.24 +/- A 0.57). Carriers of the variant rs17782313 exhibit increased triglyceride levels (108 +/- A 48 vs. 119 +/- A 54, p = 0.034) and an increased risk of hypertriglyceridemia (OR 1.985, 95 % CI 1.288-3.057, p = 0.002). There was no association of the SNP rs12970134 with clinical, metabolic, or nutritional parameters. The variant rs12970134 and rs17782313 did not influence food intake or the presence of BE. The variant rs17782313 is associated with an increased risk of hypertriglyceridemia in obese children and adolescents.
conferenceObject POLYMORPHISM RS17782313 IN MC4R GENE IS NOT ASSOCIATED WITH BINGE EATING BEHAVIOR IN A BRAZILIAN COHORT OF OVERWEIGHT CHILDREN(2013) FERNANDES, A. E.; MELO, M. E.; FUJIWARA, C. T. H.; SANTOS, A.; CERCATO, C.; HALPERN, A.; MANCINI, M.- Genetic Variation in CD36 Is Associated with Decreased Fat and Sugar Intake in Obese Children and Adolescents(2016) PIOLTINE, Marina B.; MELO, Maria Edna de; SANTOS, Aritania; MACHADO, Alisson D.; FERNANDES, Ariana E.; FUJIWARA, Clarissa T.; CERCATO, Cintia; MANCINI, Marcio C.Background/Aims: Taste is recognized as an important predictor of food choices. Thus, polymorphisms in genes encoding taste receptors may explain the variability in food preference and intake. Here, we aimed to determine whether genetic variation in the CD36 gene affects food intake and risk of obesity. Methods: A cross-sectional study was conducted with obese Brazilian children and adolescents (n = 466; BMI-for-age z-score [zBMI] 3.29 +/- 0.61) and normal-weight children (n = 114; zBMI -0.11 +/- 0.7). To assess the obesity risk according to genotypes, a logistic regression adjusted for age and gender was performed. Two 24-h food recalls assessed total energy (kcal/day) and macronutrient (% kcal and g/day) intake, consumption of sweet and fatty tasting foods (portion and g/day), as well as the most commonly consumed foods (mL or g/day). The food portion sizes were measured according to Brazilian guidelines. The genetic variant rs1761667 (A/G) in CD36 was genotyped by real-time PCR. Results: We found no relationship between rs1761667 genotypes and obesity risk. A significant genetic association between CD36 genotype and fat intake was observed for the A allele of rs1761667, which was associated with a decreased intake of total fat (g/day) (p = 0.01), polyunsaturated and monounsaturated fatty acids (% kcal and g/day), total sugars (g/day) (p = 0.01), fatty foods (portion and g/day) (p < 0.001 for both), and vegetable oils (mL/day) (p = 0.02) only in obese subjects. No differences were found between normal-weight children. Conclusion: The A allele of the rs1761667 single nucleotide polymorphism in CD36 is associated with decreased fat and sugar intake in obese children and adolescents. (C) 2017 S. Karger AG, Basel
- Genetic Variations in Sweet Taste Receptor Gene Are Related to Chocolate Powder and Dietary Fiber Intake in Obese Children and Adolescents(2018) PIOLTINE, Marina B.; MELO, Maria Edna de; SANTOS, Aritania S.; MACHADO, Alisson D.; FERNANDES, Ariana E.; FUJIWARA, Clarissa T.; CERCATO, Cintia; MANCINI, Marcio C.Childhood obesity is a major public health problem. It has a direct impact on the quality of life of children and adolescents, as well as on their future risk of developing chronic diseases. Dietary patterns rich in fats and sugars and lacking dietary fibers, vitamins, and minerals, as well as lack of physical exercise have been associated with the rise of obesity prevalence. However, factors that contribute to the preference for foods rich in these nutrients are not well established. Taste is recognized as an important predictor of food choices, and polymorphisms in taste-related genes may explain the variability of taste preference and food intake. The aim of this research is to evaluate the influence of polymorphisms of the sweet taste receptor gene TAS1R2 on diet and metabolic profile in obese children and adolescents. A cross-sectional study with 513 obese children and adolescents and 135 normal-weight children was carried out. A molecular study was performed for the single nucleotide polymorphisms (SNPs) rs9701796 and rs35874116 of TAS1R2, and dietary intake, anthropometric parameters (weight, height, waist circumference, waist-to-height ratio (WHtR)), and metabolic profile (including fasting glucose, insulin, triglyceride, high-density lipoprotein (HDL)-cholesterol, and leptin levels) were analyzed. The variant rs9701796 was associated with increased waist-height ratio, as well as with a higher chocolate powder intake in obese children. The variant rs35874116 was associated with a lower dietary fiber intake. In conclusion, there was no relationship between genotypes and risk of obesity. Obese adolescents carrying the serine allele of SNP rs9701796 in TAS1R2 showed higher waist-to-height ratio and chocolate powder intake, whereas those carrying the valine allele of SNP rs35874116 in TAS1R2 were characterized by lower dietary fiber intake.