ANA MARIA DE MENDONCA COELHO
Projetos de Pesquisa
Unidades Organizacionais
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina
11 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 11
- Trisulfate Disaccharide Decreases Calcium Overload and Protects Liver Injury Secondary to Liver Ischemia/Reperfusion(2016) VASQUES, Enio Rodrigues; CUNHA, Jose Eduardo Monteiro; COELHO, Ana Maria Mendonca; SAMPIETRE, Sandra N.; PATZINA, Rosely Antunes; ABDO, Emilio Elias; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo Andrade; GODOY, Carlos M. G.; RODRIGUES, Tiago; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.Background Ischemia and reperfusion (I/R) causes tissue damage and intracellular calcium levels are a factor of cell death. Sodium calcium exchanger (NCX) regulates calcium extrusion and Trisulfated Disaccharide (TD) acts on NCX decreasing intracellular calcium through the inhibition of the exchange inhibitory peptide (XIP). Objectives The aims of this research are to evaluate TD effects in liver injury secondary to I/R in animals and in vitro action on cytosolic calcium of hepatocytes cultures under calcium overload. Methods Wistar rats submitted to partial liver ischemia were divided in groups: Control: (n = 10): surgical manipulation with no liver ischemia; Saline: (n = 15): rats receiving IV saline before reperfusion; and TD: (n = 15): rats receiving IV TD before reperfusion. Four hours after reperfusion, serum levels of AST, ALT, TNF-alpha, IL-6, and IL-10 were measured. Liver tissue samples were collected for mitochondrial function and malondialdehyde (MDA) content. Pulmonary vascular permeability and histologic parameters of liver were determined. TD effect on cytosolic calcium was evaluated in BRL3A hepatic rat cell cultures stimulated by thapsigargin pre and after treatment with TD. Results AST, ALT, cytokines, liver MDA, mitochondrial dysfunction and hepatic histologic injury scores were less in TD group when compared to Saline Group (p<0.05) with no differences in pulmonary vascular permeability. In culture cells, TD diminished the intracellular calcium raise and prevented the calcium increase pre and after treatment with thapsigargin, respectively. Conclusion TD decreases liver cell damage, preserves mitochondrial function and increases hepatic tolerance to I/R injury by calcium extrusion in Ca2+ overload situations.
- THE M-RNA, EXPRESSION OF SERCA2 AND NCX1 IN THE PROCESS OF PHARMACOLOGICAL CELL PROTECTION IN EXPERIMENTAL ACUTE PANCREATITIS INDUCED BY TAUROCHOLATE(2018) VASQUES, Enio Rodrigues; CUNHA, Jose Eduardo Monteiro; KUBRUSLY, Marcia Saldanha; COELHO, Ana Maria; SANPIETRI, Sandra N.; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo A.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto CarneiroBackground: Intracellular calcium overload is known to be a precipitating factor of pancreatic cell injury in acute pancreatitis (AP). Intracellular calcium homeostasis depends of Plasmatic Membrane Calcium ATPase (PMCA), Sarcoplasmic Endothelial Reticulum Calcium ATPase 2 (SERCA 2) and the Sodium Calcium Exchanger (NCX1). The antioxidant melatonin (Mel) and Trisulfate Disaccharide (TD) that accelerates NCX1 action could reduce the cell damage determined by the AP. Aim: To evaluate m-RNA expressions of SERCA2 and NCX1 in acute pancreatitis induced by sodium taurocholate in Wistar rats pre-treated with melatonin and/or TD. Methods: Wistar rats were divided in groups: 1) without AP; 2) AP without pre-treatment; 3) AP and Melatonin; 4) AP and TD; 5) AP and Melatonin associated to TD. Pancreatic tissue samples were collected for detection of SERCA2 and NCX1 m-R NA levels by polymerase chain reaction (PCR). Results: Increased m-RNA expression of SERCA2 in the melatonin treated group, without increase of m-RNA expression of the NCX1. The TD did not affect levels of SERCA2 and NCX1 m-RNA expressions. The combined melatonin and TD treatment reduced the m-RNA expression of SERCA2. Conclusions: The effect of melatonin is restricted to increased m-RNA expression of SERCA2. Although TD does not affect gene expression, its action in accelerating calcium exchanger function can explain the slightest expression of SERCA2 m-RNA when associated with Melatonin, perhaps by a joint action of drugs with different and but possibly complementary mechanisms.
conferenceObject Heparin Fragments Effects in Liver Injury Secondary to Liver Ischemia/Reperfusion (I/R)(2014) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; ABDO, Emilio E.; SAMPIETRE, Sandra N.; NADER, Helena B.; TERSARIOL, Ivarne S.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.conferenceObject Trisulfated Disaccharide Decreases Intracellular Calcium Concentration in Hepatocytes Under Calcium Overload Situation(2015) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; CHAIB, Eleazar; NADER, Helena B.; TERSARIOL, Ivarne S.; ALBUQUERQUE, Luiz C. D.; RODRIGUES, TiagoconferenceObject Aged-Depend Vulnerability to Experimental Acute Pancreatitis Is Associated With Previous Liver Mitochondrial Damage(2015) COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; MACHADO, Marcel C.; CUNHA, Jose Eduardo M.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.conferenceObject THE MRNA EXPRESSION OF SERCA AND NCX IN ACUTE PANCREATITIS MODEL UNDER MELATONIN AND TRISULFATE DISSACHARIDE ACTION(2017) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; KUBRUSLY, Marcia S.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; RODRIGUES, Tiago; NADER, Helena; TERSARIOL, Ivarne S.; LIMA, Marcelo A.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.conferenceObject Local and Systemic Effects of Aging on Acute Pancreatitis(2013) COELHO, Ana Maria M.; MACHADO, Marcel C.; SAMPIETRE, Sandra N.; MOLAN, Nilza A.; HEIJDEN, Inneke M. van der; CUNHA, Jose Eduardo M.; D'ALBUQUERQUE, Luiz C.- Local and systemic effects of aging on acute pancreatitis(2019) COELHO, Ana Maria Mendonca; MACHADO, Marcel Cerqueira Cesar; SAMPIETRE, Sandra Nassa; SILVA, Fabiano Pinheiro da; CUNHA, Jose Eduardo Monteiro; D'ALBUQUERQUE, Luiz Augusto CarneiroBackground: /Objectives: Evaluation of the local and systemic effects of aging on the severity of acute pancreatitis (AP) in an experimental rat model in elderly animals. Methods: AP was induced in Wistar rats by intraductal 2.5% taurocholate injection and divided into two groups: Young (3 month old) and Aged (18 month old). Two and 24 h after AP induction blood samples were collected for determinations of amylase, AST, ALT, urea, creatinine, glucose, and of plasma I-FABP. TNF-alpha and IL-6 levels were determined in serum and ascitic fluid. Liver mitochondrial function and malondialdehyde (MDA) contents, pancreas histological analysis, and pulmonar myeloperoxidade (MPO) activity were performed. Bacterial translocation was evaluated by bacterial cultures of pancreas. Results: A significant increase in serum amylase, AST, ALT, urea, creatinine, glucose, I-FABP, and IL-6 levels, and a reduction in serum and ascitic fluid TNF-alpha levels were observed in the aged group compared to the young group. Liver mitochondrial dysfunction, MDA contents, and pulmonary MPO activity were increased in the Aged AP group compared to the Young AP group. Positive bacterial cultures obtained from pancreas tissue in aged group were significantly increased compared to the young group. Acinar necrosis was also increased in aged AP group when compared to young AP group. Conclusion: Aging worsens the course of acute pancreatitis evidenced by increased local and systemic lesions and increased bacterial translocation.
conferenceObject Low Molecular Weight Heparin Fragment Decreases Intracellular Calcium in Human Hepatocarcinoma Cells Under Calcium Overload(2016) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; ABDO, Emilio E.; NADER, Helena B.; TERSARIOL, Ivarne S.; LIMA, Marcelo A.; D'ALBUQUERQUE, Luiz Augusto C.; CHAIB, Eleazar; RODRIGUES, TiagoconferenceObject ACTION OF HEPARIN FRAGMENT ON CALCIUM DYNAMICS AND CELL MORTALITY IN A PANCREATIC CELL CULTURE UNDER TAUROCHOLATE EXPOSITION(2017) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; KUBRUSLY, Marcia S.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.; NADER, Helena; LIMA, Marcelo A.; TERSARIOL, Ivarne S.; RODRIGUES, Tiago