IARA MORENO LINHARES
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
10 resultados
Resultados de Busca
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- Inhibition of Autophagy by Sera From Pregnant Women(2013) KANNINEN, Tomi T.; RAMOS, Bruna Ribeiro de Andrade; JAFFE, Shirlee; BONGIOVANNI, Ann Marie; LINHARES, Iara Moreno; RENZO, Gian Carlo Di; WITKIN, Steven S.Autophagy is a process that maintains homeostasis by eliminating senescent or damaged intracellular organelles and proteins. Its role in pregnancy has been scarcely studied. We compared the influence of sera from pregnant and nonpregnant women on autophagy induction. Peripheral blood mononuclear cells (PBMCs) were incubated with sera from 35 pregnant or nonpregnant women in the presence or absence of the autophagy inducer, rapamycin. After 48 hours, the cells were assayed for p62, a cytoplasmic protein essential for autophagy induction. Its concentration in the cytoplasm is inversely proportional to the level of autophagy induction. Sera were tested for immune mediators by enzyme-linked immunosorbent assay. Median (range) p62 concentrations were 6.7 ng/mL (1.1-22.7) for PBMCs incubated with pregnancy sera versus 2.5 ng/mL (0.8-7.7) for nonpregnant sera (P < .0001). In the presence of rapamycin, median p62 levels were 1.3 ng/mL (<0.1-4.9) with pregnancy sera, when compared to 0.6 ng/mL (<0.1-3.3) with control sera (P = .0191). Among the pregnant patients, the p62 level was inversely proportional to the results of a 50-g glucose challenge test (r = -.5630, P = .0005). Sera from pregnant women had elevated levels of insulin-like growth factor 1 (IGF-1), interleukin 13 (IL-13), and transforming growth factor 1 (TGF-1). Autophagy during pregnancy may be inhibited by IGF-1, IL-13, and/or TGF-1 and may influence insulin resistance.
- Amylase in Vaginal Fluid: Association With Conditions Favorable to Dominance of Lactobacillus(2015) NASIOUDIS, Dimitrios; BEGHINI, Joziani; BONGIOVANNI, Ann Marie; GIRALDO, Paulo C.; LINHARES, Iara M.; WITKIN, Steven S.Vaginal glycogen is degraded by host -amylase and then converted to lactic acid by Lactobacilli. This maintains the vaginal pH at 4.5 and prevents growth of other bacteria. Therefore, host -amylase activity may promote dominance of Lactobacilli. We evaluated whether the -amylase level in vaginal fluid is altered in women with bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC) and whether its concentration was associated with levels of lactic acid isomers and host mediators. Vaginal fluid was obtained from 43 women with BV, 50 women with VVC, and 62 women with no vulvovaginal disorders. Vaginal fluid concentrations of -amylase, secretory leukocyte protease inhibitor (SLPI), hyaluronan, hyaluronidase-1, -defensin, and elafin were measured by enzyme-linked immunosorbent assay (ELISA). Vaginal concentrations of neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinase (MMP) 8, and d- and l-lactic acid levels in these patients were previously reported. The median vaginal fluid -amylase level was 1.83 mU/mL in control women, 1.45 mU/mL in women with VVC, and 1.07 mU/mL in women with BV. Vaginal levels of -amylase were correlated with d-lactic acid (P = .003) but not with l-lactic acid (P > .05) and with SLPI (P < .001), hyaluronidase-1 (P < .001), NGAL (P = .001), and MMP-8 (P = .005). The exfoliation of glycogen-rich epithelial cells into the vaginal lumen by hyaluronidase-1 and MMP-8 may increase glycogen availability and promote -amylase activity. The subsequent enhanced availability of glycogen breakdown products would favor proliferation of Lactobacilli, the primary producers of d-lactic acid in the vagina. Concomitant production of NGAL and SLPI would retard growth of BV-related bacteria.
conferenceObject Alpha Amylase Levels in Vaginal Fluid and Its Relation To Anti-Bacterial and Membrane Degradation Mediators.(2015) NASIOUDIS, Dimitrios; BEGHINI, Joziani; BONGIOVANNI, Ann Marie; GIRALDO, Paulo C.; LINHARES, Iara M.; WITKIN, Steven S.conferenceObject Mannose-Binding Lectin Gene Polymorphism and Chlamydia Trachomatis Antibodies in Brazilian Women with Tubal Occlusion.(2019) VINAGRE, Joao G.; WITKIN, Steven S.; FUKAZAWA, Eiko I.; ROBIAL, Renata; BARACAT, Edmund C.; LINHARES, Iara M.conferenceObject Mannose-Binding Lectin (MBL) and Interferon Gamma (IFNg) Gene Polymorphisms Influence Susceptibility To Blocked Fallopian Tubes(2015) CHERVENAK, Joseph; PIRES, Fatima M.; LINHARES, Iara M.; NORMAND, Neil; RIBEIRO, Sergio C.; BARACAT, Edmund C.; WITKIN, Steven S.- Atypical Autophagy Induction by Sera from Pregnant Women with Preeclampsia(2014) KANNINEN, Tomi T.; JAYARAM, Aswathi; JAFFE-LIFSHITZ, Shirlee; SISTI, Giovanni; LINHARES, Iara M.; WITKIN, Steven S.
- Torquetenovirus Titer in Vaginal Secretions from Pregnant and Postpartum Women: Association with Absence ofLactobacillus crispatusand Levels of Lactic Acid and Matrix Metalloproteinase-8(2020) TOZETTO-MENDOZA, Tania R.; BONGIOVANNI, Ann Marie; MINIS, Evelyn; LINHARES, Iara M.; BOESTER, Allison; FREIRE, Wilton S.; LIMA, Silvia H.; OLIVEIRA, Natan P. G. de; MENDES-CORREA, Maria C.; FORNEY, Larry J.; WITKIN, Steven S.Vaginal samples from women with term deliveries were tested for torquetenovirus (TTV) by gene amplification, matrix metalloproteinase (MMP)-8 and D- and L-lactic acid by ELISA, and microbiome composition by analysis of the bacterial 16S ribosomal RNA gene. TTV was detected in 43.2%, 31.5%, and 41.4% of first trimester, third trimester, and postpartum samples, respectively. The viral titer was higher in postpartum than in the first (p = 0.0018) or third (p = 0.0013) trimester. The mean gestational age at delivery was lower in women positive for TTV in their first trimester (p = 0.0358). In the first and third trimester, the MMP-8 level was higher if TTV was also present (p < 0.0091). The D-lactic acid level was lower in first trimester samples if TTV was present (p = 0.0334).Lactobacillus crispatusdominance in first and third trimester samples was higher when TTV was absent (p < 0.0033). We conclude that TTV is present in the vagina in many women with normal pregnancy outcomes and that its occurrence is associated with a lack ofL. crispatusdominance, an increase in vaginal MMP-8 and a decrease in D-lactic acid.
conferenceObject Influence of Recurrent Vulvovaginal Candidiasis on Quality of Life.(2019) FUKAZAWA, Eiko I.; WITKIN, Steven S. Steven Sol; ROBIAL, Renata; VINAGRE, Joao G.; BARACAT, Edmund C.; LINHARES, Iara M.- The Medium Is the Message: Defining a ""Normal"" Vaginal Microbiome in Healthy Reproductive-Age Women(2023) WITKIN, Steven S.; LINHARES, Iara MorenoThe microbial composition of the human vagina differs from that of all other mammals, likely as a consequence of the unique composition of vaginal constituents that promote the selective maintenance and proliferation of distinct bacterial species. Similarly, variations between individual healthy women in genetic, environmental, and medical variables also modify the vaginal lumen composition. The direction and magnitude of immune reactions to microorganisms present in the vagina, responses to stress and non-infectious stimuli, coupled with medical and pregnancy history and environmental exposures, can greatly differ between women. Adaptations to both internal and external pressures will determine the ability of select resident vaginal bacteria to numerically dominate and, therefore, the definition of a ""normal"" vaginal microbiota will substantially differ between individual healthy women.
- Neutrophil Gelatinase-Associated Lipocalin Concentration in Vaginal Fluid: Relation to Bacterial Vaginosis and Vulvovaginal Candidiasis(2015) BEGHINI, Joziani; GIRALDO, Paulo C.; LINHARES, Iara M.; LEDGER, William J.; WITKIN, Steven S.Objective: Neutrophil gelatinase-associated lipocalin (NGAL) is a component of innate immunity that prevents iron uptake by microorganisms. We evaluated whether NGAL was present in vaginal fluid and whether concentrations were altered in women with bacterial vaginosis (BV) or vulvovaginal candidiasis (VVC). Methods: Vaginal secretions from 52 women with VVC, 43 with BV, and 77 healthy controls were assayed by enzyme-linked immunosorbent assay for NGAL and for concentrations of l-lactic acid. Results: The median concentration of NGAL in vaginal fluid was significantly higher in control women (561 pg/mL) than in women with BV (402 pg/mL; P = .0116) and lower in women with VVC (741 pg/mL; P = .0017). Median lactic acid levels were similar in controls (0.11 mmol/L) and women with VVC (0.13 mmol/L) and were lower in women with BV (0.02 mmol/L; P < .0001). The NGAL and lactic acid concentrations were highly correlated (P < .0001). Conclusion: A decrease in Lactobacilli and/or lactic acid plus the absence of leukocytes results in lower vaginal NGAL levels that might facilitate the growth of bacteria associated with BV.