CARLOS AUGUSTO GONCALVES PASQUALUCCI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
ATCIENT-50, SVOC
LIM/22 - Laboratório de Patolologia Cardiovascular, Hospital das Clínicas, Faculdade de Medicina - Líder
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Determination of antidepressants in whole blood using hollow-fiber liquid-phase microextraction and gas chromatography-mass spectrometry(2014) SANTOS, Marcelo Filonzi dos; FERRI, Caio Caleiras; SEULIN, Saskia Carolina; LEYTON, Vilma; PASQUALUCCI, Carlos Augusto Goncalves; MUNOZ, Daniel Romero; YONAMINE, MauricioA hollow-fiber liquid-phase microextraction (HF-LPME), used in three-phase mode, and combined with gas chromatography-mass spectrometry (GC-MS), was developed to quantify antidepressants and their major metabolites (amitriptyline, nortriptyline, imipramine, desipramine, clomipramine, desmethylclomipramine, fluoxetine, and norfluoxetine) in whole blood samples, using their deuterated analogs as internal standards. The HF-LPME system comprised a disposable 8-cm polypropylene porous hollow fiber, 4.0 ml of sample solution (0.5 ml of blood added to 3.5 ml of 0.1 M NaOH: donor phase), dodecane (organic phase), and 0.1 M formic acid (acceptor phase) for extraction. After stirring the system, the acceptor phase was evaporated under a nitrogen stream and resuspended in 30 mu l of methanol. Derivatization was not required. A 2.0-mu l aliquot of this solution was injected into a GC-MS system. The method was validated after the optimization of several parameters that may influence the extraction efficiency. The limits of quantification for all antidepressants were below the therapeutic levels (20.0 ng/ml). The average intraday and interday precisions were within 9.7 and 9.8 %, respectively, for all analytes. The calibration curves were linear in the concentration range of 20-1,200 ng/ml. The developed method was applied to seven actual postmortem samples. Tricyclic antidepressants were detected in all of the analyzed cases. To our knowledge, this is the first demonstration of usefulness of HF-LPME for analysis of antidepressants in postmortem forensic cases.
- Differences between postmortem computed tomography and conventional autopsy in a stabbing murder case(2014) ZERBINI, Talita; SILVA, Luiz Fernando Ferraz da; FERRO, Antonio Carlos Goncalves; KAY, Fernando Uliana; AMARO JUNIOR, Edson; PASQUALUCCI, Carlos Augusto Goncalves; SALDIVA, Paulo Hilario do NascimentoOBJECTIVE: The aim of the present work is to analyze the differences and similarities between the elements of a conventional autopsy and images obtained from postmortem computed tomography in a case of a homicide stab wound. METHOD: Comparison between the findings of different methods: autopsy and postmortem computed tomography. RESULTS: In some aspects, autopsy is still superior to imaging, especially in relation to external examination and the description of lesion vitality. However, the findings of gas embolism, pneumothorax and pulmonary emphysema and the relationship between the internal path of the instrument of aggression and the entry wound are better demonstrated by postmortem computed tomography. CONCLUSIONS: Although multislice computed tomography has greater accuracy than autopsy, we believe that the conventional autopsy method is fundamental for providing evidence in criminal investigations.
conferenceObject High prevalence of TDP-43 in Asians older adults: a clinicopathological study on a population-based sample(2014) NASCIMENTO, C.; RODRIGUEZ, R. D.; LEITE, R. P.; SUEMOTO, C. K.; ZATZ, M.; PASQUALUCCI, C. A.; FILHO, W. J.; GRINBERG, L. T.- A novel approach for integrative studies on neurodegenerative diseases in human brains(2014) THEOFILAS, Panos; POLICHISO, Livia; WANG, Xuehua; LIMA, Luzia C.; ALHO, Ana T. L.; LEITE, Renata E. P.; SUEMOTO, Claudia K.; PASQUALUCCI, Carlos A.; JACOB-FILHO, Wilson; HEINSEN, Helmut; GRINBERG, Lea T.Despite a massive research effort to elucidate Alzheimer's disease (AD) in recent decades, effective treatment remains elusive. This failure may relate to an oversimplification of the pathogenic processes underlying AD and also lack of understanding of AD progression during its long latent stages. Although evidence shows that the two specific neuropathological hallmarks in AD (neuronal loss and protein accumulation), which are opposite in nature, do not progress in parallel, the great majority of studies have focused on only one of these aspects. Furthermore, research focusing on single structures is likely to render an incomplete picture of AD pathogenesis because as AD involves complete brain networks, potential compensatory mechanisms within the network may ameliorate impairment of the system to a certain extent. Here, we describe an approach for enabling integrative analysis of the dual-nature lesions, simultaneously, in all components of one of the brain networks most vulnerable to AD. This approach is based on significant development of methods previously described mainly by our group that were optimized and complemented for this study. It combines unbiased stereology with immunohistochemistry and immunofluorescence, making use of advanced graphics computing for three-dimensional (3D) volume reconstructions. Although this study was performed in human brainstem and focused in AD, it may be applied to the study of any neurological disease characterized by dual-nature lesions, in humans and animal models. This approach does not require a high level of investment in new equipment and a significant number of specimens can be processed and analyzed within a funding cycle.
- Discrimination of prostate carcinoma from benign prostate tissue fragments in vitro by estimating the gross biochemical alterations through Raman spectroscopy(2014) SILVEIRA JR., Landulfo; LEITE, Katia Ramos M.; SILVEIRA, Fabricio Luiz; SROUGI, Miguel; PACHECO, Marcos Tadeu T.; ZANGARO, Renato Amaro; PASQUALUCCI, Carlos AugustoRaman spectroscopy has been proposed for detecting biochemical alterations in prostate cancer (PrCa) compared to benign prostate tissue in in vitro fragments from surgery for diagnostic purposes. Freezer-stored fragments of human prostate tissues were unfrozen and submitted to Raman spectroscopy with a dispersive spectrometer (830-nm and 200-mW laser parameters, 30-s exposure time). Fragments were fixed and submitted to histopathology to grade PrCa according to Gleason score. A total of 160 spectra were taken from 32 samples (16 benign tissues and 16 PrCa tissues). The relative concentrations of selected biochemicals were estimated using a least-squares fitting model applied to the spectra of pure compounds and the tissue spectrum. A discrimination model was developed employing the most statistically relevant compounds with capability of separating PrCa from benign tissues. The fitting model revealed that actin, hemoglobin, elastin, phosphatidylcholine, and water are the most important biochemicals to discriminate prostate depending on the Gleason score. A discrimination based on Euclidean distance using the relative concentrations of phosphatidylcholine and water showed the higher accuracy of 74 % to discriminate PrCa from benign tissue. Raman spectroscopy is an analytical technique with possibility for identifying biochemical constitution of prostate and could be used for diagnostic purposes.
- Sexual Dimorphism in the Human Olfactory Bulb: Females Have More Neurons and Glial Cells than Males(2014) OLIVEIRA-PINTO, Ana V.; SANTOS, Raquel M.; COUTINHO, Renan A.; OLIVEIRA, Lays M.; SANTOS, Glaucia B.; ALHO, Ana T. L.; LEITE, Renata E. P.; FARFEL, Jose M.; SUEMOTO, Claudia K.; GRINBERG, Lea T.; PASQUALUCCI, Carlos A.; JACOB-FILHO, Wilson; LENT, RobertoSex differences in the human olfactory function reportedly exist for olfactory sensitivity, odorant identification and memory, and tasks in which odors are rated based on psychological features such as familiarity, intensity, pleasantness, and others. Which might be the neural bases for these behavioral differences? The number of cells in olfactory regions, and especially the number of neurons, may represent a more accurate indicator of the neural machinery than volume or weight, but besides gross volume measures of the human olfactory bulb, no systematic study of sex differences in the absolute number of cells has yet been undertaken. In this work, we investigate a possible sexual dimorphism in the olfactory bulb, by quantifying postmortem material from 7 men and 11 women (ages 55-94 years) with the isotropic fractionator, an unbiased and accurate method to estimate absolute cell numbers in brain regions. Female bulbs weighed 0.132 g in average, while male bulbs weighed 0.137 g, a non-significant difference; however, the total number of cells was 16.2 million in females, and 9.2 million in males, a significant difference of 43.2%. The number of neurons in females reached 6.9 million, being no more than 3.5 million in males, a difference of 49.3%. The number of non-neuronal cells also proved higher in women than in men: 9.3 million and 5.7 million, respectively, a significant difference of 38.7%. The same differences remained when corrected for mass. Results demonstrate a sex-related difference in the absolute number of total, neuronal and non-euronal cells, favoring women by 40-50%. It is conceivable that these differences in quantitative cellularity may have functional impact, albeit difficult to infer how exactly this would be, without knowing the specific circuits cells make. However, the reported advantage of women as compared to men may stimulate future work on sex dimorphism of synaptic microcircuitry in the olfactory bulb.