SONIA DE FATIMA SOTO YOSHIDA
Projetos de Pesquisa
Unidades Organizacionais
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina
9 resultados
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conferenceObject Side effects of tacrolimus upon airway epithelial tissue(2013) SILVA, Maristela Prado e; SOTO, Sonia; ALMEIDA, Francine; LIMONETE, Tatiana; PARRA, Edwin; PEGO-FERNANDES, Paulo; JATENE, Fabio; PAZETTI, RogerioconferenceObject Comparison of two immunosuppressant triple therapies on airway mucociliary clearance in rats(2012) SILVA, Maristela Prado e; SOTO, Sonia; ALMEIDA, Francine; LIMONETE, Tatiana; PARRA, Edwin; PEGO-FERNANDES, Paulo; JATENE, Fabio; PAZETTI, Rogerio- Immunosuppression Effects on Airway Mucociliary Clearance: Comparison Between Two Triple Therapies(2013) SILVA, Maristela Prado e; SOTO, Sonia F.; ALMEIDA, Francine M.; LIMONETE, Tatiana T. K.; PARRA, Edwin R. C.; JATENE, Fabio B.; PEGO-FERNANDES, Paulo M.; PAZETTI, RogerioBackground. Tacrolimus and mycophenolate have now become the most widely used combination for maintenance immunosuppressive regimens after lung transplantation in comparison with cyclosporine and azathioprine. However, limited information is available with respect to their effects on cells, other than those from the immunologic compartment. We hypothesized that different triple therapies could have different effects on airway mucociliary clearance, playing an important role in respiratory infections observed after lung transplantation. Methods. Ninety rats were assigned to three groups (n = 30 each): control = vehicle, therapy 1 = tacrolimus + mycophenolate + prednisone, and therapy 2 = cyclosporine + azathioprine + prednisone. After 7, 15, or 30 days of treatment by gavage, the animals were killed and the following parameters were studied: mucus transportability, ciliary beating frequency, mucociliary transport velocity, and neutral and acid mucus production. Results. There was a significant decrease in ciliary beating frequency, mucociliary transport velocity, and neutral mucus production in all immunosuppressed animals; indeed, both therapies, mainly therapy 1, caused an increase in acid mucus production for as long as 15 days of treatment. Conclusions. Both triple therapies impaired airway mucociliary clearance of rats, but therapy 1 had a more deleterious effect. These data suggest that these undesirable effects can contribute to the high incidence of respiratory infections observed in patients undergoing lung transplantation. (C) 2013 by The Society of Thoracic Surgeons
conferenceObject Tacrolimus increases inflammatory cells in lung(2014) ALMEIDA, Francine Maria; SILVA, Maristela Prado e; SOTO, Sonia Fatima; CORREIA, Aristides Tadeu; PEGO-FERNANDES, Paulo; PAZETTI, Rogerio- The effects of individually ventilated cages on the respiratory systems of male and female Wistar rats from birth until adulthood(2017) MARCHESI, Guilherme DAprile; SOTO, Sonia de Fatima; CASTRO, Isac de; RODRIGUES, Thiago Guimaraes; MORIYA, Henrique Takachi; ALMEIDA, Francine Maria de; PAZETTI, Rogerio; HEIMANN, Joel Claudio; FURUKAWA, Luzia Naoko ShinoharaOBJECTIVE: To evaluate the respiratory systems of male and female rats maintained in individually ventilated cages (IVCs) from birth until adulthood. METHODS : Female Wistar rats were housed in individually ventilated cages or conventional cages (CCs) and mated with male Wistar rats. After birth and weaning, the male offspring were separated from the females and kept in cages of the same type until 12 weeks of age. RESULTS: The level of food consumption was lower in male offspring (IVC=171.7 +/- 9; CC=193.1 +/- 20) than in female offspring (IVC=100.6 +/- 7; CC=123.4 +/- 0.4), whereas the water intake was higher in female offspring (IVC=149.8 +/- 11; CC=99.2 +/- 0) than in male offspring (IVC=302.5 +/- 25; CC=249.7 +/- 22) at 11 weeks of age when housed in IVCs. The cage temperature was higher in individually ventilated cages than in conventional cages for both male (IVCs=25.9 +/- 0.5; CCs=22.95 +/- 0.3) and female (IVCs=26.2 +/- 0.3; CCs=23.1 +/- 0.3) offspring. The respiratory resistance (IVC=68.8 +/- 2.8; CC=50.6 +/- 3.0) and elastance (IVC=42.0 +/- 3.9; CC=32.4 +/- 2.0) at 300 mm/kg were higher in the female offspring housed in ventilated cages. The ciliary beat values were lower in both the male (IVCs=13.4 +/- 0.2; CC=15 +/- 0.4) and female (IVC=13.5 +/- 0.4; CC=15.9 +/- 0.6) offspring housed in individually ventilated cages than in those housed in conventional cages. The total cell (IVC=117.5 +/- 9.7; CC=285.0 +/- 22.8), neutrophil (IVC=13.1 +/- 4.8; CC=75.6 +/- 4.1) and macrophage (IVC=95.2 +/- 11.8; CC=170.0 +/- 18.8) counts in the bronchoalveolar lavage fluid were lower in the female offspring housed in individually ventilated cages than in those housed in conventional cages. CONCLUSIONS : The environmental conditions that exist in individually ventilated cages should be considered when interpreting the results of studies involving laboratory animals. In this study, we observed gender dimorphism in both the water consumption and respiratory mechanics of rats kept in ventilated cages.
- Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury(2016) ALMEIDA, Francine Maria; OLIVEIRA-JUNIOR, Manoel Carneiro; SOUZA, Renato Aparecido; PETRONI, Ricardo Costa; SOTO, Sonia Fatima; SORIANO, Francisco Garcia; CARVALHO, Paulo Tarso Camillo de; ALBERTINI, Regiane; DAMACENO-RODRIGUES, Nilsa Regina; LOPES, Fernanda Degobbi Tenorio Quirino Santos; CASTRO-FARIA-NETO, Hugo Caire; MARTINS, Milton Arruda; DOLHNIKOFF, Marisa; PAZETTI, Rogerio; VIEIRA, Rodolfo PaulaBACKGROUND: Creatine (Cr) is a dietary supplement that presents beneficial effects in experimental models of heart and brain ischemia and reperfusion (I/R) injury. It can improve adenosine 5'-triphosphate generation and reduce cell damage This study evaluated the effects of Cr supplementation in a model of lung PR. METHODS: Forty male Wistar rats were divided into 4 groups: sham operated, Cr+sham, I/R, and Cr+I/R. We investigated the effects of 5 days of Cr supplementation (0.5 g/kg/day by gavage) before left pulmonary artery ischemia (90 minutes) and reperfusion (120 minutes) on pulmonary and systemic response. RESULTS: Cr inhibited the I/R-induced increase in exhaled nitric oxide (p < 0.05), total cells (p < 0.01), and neutrophils (p < 0.001) in bronchoalveolar lavage fluid and in the systemic circulation (p < 0.001). The levels of interleulcin-1 beta (p < 0.05), tissue damping, and tissue elastance (p < 0.05) were also minimized Cr also inhibited pulmonary edema formation (total proteins in bronchoalveolar lavage fluid, p < 0.001; histologic edema index, p < 0.001) and neutrophils accumulation in lung tissue (p < 0.001). As possible mechanisms underlying Cr effects, we observed a reduced expression of caspase 3 (p < 0.05), reduced expression of Toll-like receptor (TLR) 4, and increased expression of TLR7 in lung tissue (p < 0.001). CONCLUSIONS: Cr supplementation presents pulmonary and systemic protective effects in acute lung injury induced by I/R in rats. These beneficial effects seem to be related to the anti-inflammatory and antioxidant properties of Cr and modulation of TLRs.
conferenceObject Effects of two immunosuppressive triple therapies on inflammatory cells in lung(2014) ALMEIDA, Francine Maria; SILVA, Maristela Prado e; SOTO, Sonia Fatima; CORREIA, Aristides Tadeu; PEGO-FERNANDES, Paulo; PAZETTI, RogerioconferenceObject Creatine supplementation attenuates systemic and pulmonary effects of acute lung injury induced by pulmonary ischemia-reperfusion in rats(2012) ALMEIDA, Francine; PAZETTI, Rogerio; LOPES, Fernanda Degobbi Tenorio Quirino Santos; SOTO, Sonia Fatima; MARTINS, Milton Arruda; DOLHNIKOFF, Marisa; VIEIRA, Rodolfo PaulaconferenceObject Tacrolimus Causes Airway Mucociliary Clearance Impairment(2013) SILVA, M. P.; SOTO, S. F.; LIMONETE, T. T. K.; ALMEIDA, F. M.; JATENE, F. B.; PEGO-FERNANDES, P. M.; PAZETTI, R.Purpose: According to ISHLT Registry, the vast majority of lung transplant patients receive a tacrolimus-based maintenance immuno-suppressant therapy. However, its side effects on respiratory tract are not very well studied. We hypothesized that tacrolimus could impair airway mucociliary clearance. Methods and Materials: Twenty Wistar rats were assigned into two groups: Control (n¼10): saline solution; and TAC (n=10): tacrolimus (1 mg/kg/day). After 15 days of therapy by gavage, animals were killed and in situ mucociliary transport velocity (MCTV) and ciliary beating frequency (CBF) were measured by microscopic direct view of airway ciliated epithelium. Mucus production by goblet cells was quantified in periodic acid Schiff and alcian blue stained slides of the tracheobron-chial tissue. This study was support by São Paulo Research Foundation - Fapesp. Results: There was a significant decrease in MCTV (1.77 ± 0.50 and 0.73 ± 0.34 mm/min, Control and TAC, respectively, p<0.001) and CBF (15.06 ± 1.31 and 13.17 ± 1.47 Hertz, Control and TAC, respectively, p<0.001) in all TAC-treated animals. Indeed, tacrolimus caused an over production of mucus from airway goblet cells (12.78 ± 6.92 and 21.27 ± 7.86%, Control and TAC, respectively; p=0.007). Conclusions: Our study showed that TAC plays an important role on the impairment of the mucociliary clearance either by decreasing CBF and MCTV or by increasing mucus production. This impairment can be related with the high level of respiratory infection observed during the first 30 days after lung transplantation and must receive good attention from clinicians.