ESTER CERDEIRA SABINO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Patologia, Faculdade de Medicina - Docente
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina - Líder
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Resultados de Busca
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conferenceObject DENGUE RNA AMONG BLOOD DONORS AND RECIPIENTS DURING LARGE EPIDEMICS OF DENV-4 IN RIO DE JANEIRO AND RECIFE, BRAZIL(2013) SABINO, E.; LOUREIRO, P.; LOPES, M.; CAPUANI, L.; OLIVEIRA, C.; OLIVEIRA, L.; LINNEN, J.; LEE, T-H; PRINCE, H.; MCCLURE, C.; CHOWDHURY, D.; GONCALEZ, T.; CUSTER, B.; BRAMBILLA, D.; BUSCH, M.conferenceObject Motivation to Donate Among Granulocytes, Apheresis, and Whole Blood Donors in a Brazilian Blood Center(2013) ROCHA, P. C.; SABINO, E. C.; OLIVEIRA, C. D.; PATAVINO, G. M.; CAPUANI, L. D.; ALMEIDA-NETO, C. deconferenceObject SELF-DISCLOSED TEST SEEKING AND HIV EXPOSURE RISK AMONG BLOOD DONORS IN BRAZIL(2013) BRUHN, R.; MORENO, E.; SABINO, E.; BLATYTA, P.; CARVALHO, S. Maia Farias de; CARNEIRO-PROIETTI, A. B.; SAMPAIO, D.; CUSTER, B.; GONCALEZ, T.- Prevalence of serologic markers for hepatitis B and C viruses in Brazilian blood donors and incidence and residual risk of transfusion transmission of hepatitis C virus(2013) ALMEIDA-NETO, Cesar de; SABINO, Ester Cerdeira; LIU, Jing; BLATYTA, Paula Fraiman; MENDRONE-JUNIOR, Alfredo; SALLES, Nanci Alves; LEAO, Silvana Carneiro; WRIGHT, David J.; BASQUES, Fernando Valadares; FERREIRA, Joao Eduardo; BUSCH, Michael P.; MURPHY, Edward L.BACKGROUND: We evaluate the current prevalence of serologic markers for hepatitisB virus (HBV) and hepatitisC virus (HCV) in blood donors and estimated HCV incidence and residual transfusion-transmitted risk at three large Brazilian blood centers. STUDY DESIGN AND METHODS: Data on whole blood and platelet donations were collected from January through December 2007, analyzed by center; donor type; age; sex; donation status; and serologic results for hepatitisB surface antigen (HBsAg), antibody to hepatitisB core antigen (anti-HBc), and anti-HCV. HBV and HCV prevalence rates were calculated for all first-time donations. HCV incidence was derived including interdonation intervals that preceded first repeat donations given during the study, and HCV residual risk was estimated for transfusions derived from repeat donors. RESULTS: There were 307,354 donations in 2007. Overall prevalence of concordant HBsAg and anti-HBc reactivity was 289 per 100,000 donations and of anti-HCV confirmed reactivity 191 per 100,000 donations. There were significant associations between older age and hepatitis markers, especially for HCV. HCV incidence was 3.11 (95% confidence interval, 0.77-7.03) per 100,000 person-years, and residual risk of HCV window-phase infections was estimated at 5.0 per million units transfused. CONCLUSION: Improvement in donor selection, socioeconomic conditions, and preventive measures, implemented over time, may have helped to decrease prevalence of HBV and HCV, relative to previous reports. Incidence and residual risk of HCV are also diminishing. Ongoing monitoring of HBV and HCV markers among Brazilian blood donors should help guide improved recruitment procedures, donor selection, laboratory screening, and counseling strategies.
- Undisclosed human immunodeficiency virus risk factors identified through a computer-based questionnaire program among blood donors in Brazil(2013) BLATYTA, Paula Fraiman; CUSTER, Brian; GONCALEZ, Thelma Terezinha; BIRCH, Rebecca; LOPES, Maria Esther; FERREIRA, Maria Ines Lopes; PROIETTI, Anna Barbara Carneiro; SABINO, Ester Cerdeira; PAGE, Kimberly; ALMEIDA-NETO, Cesar deBackgroundHuman immunodeficiency virus (HIV) risk factor screening among blood donors remains a cornerstone for the safety of blood supply and is dependent on prospective donor self-disclosure and an attentive predonation interview. Audio computer-assisted structured interview (ACASI) has been shown to increase self-reporting of risk behaviors. Study Design and MethodsThis cross-sectional study was conducted between January 2009 and March 2011 at four Brazilian blood centers to identify the population of HIV-negative eligible blood donors that answered face-to-face interviews without disclosing risks, but subsequently disclosed deferrable risk factors by ACASI. Compared to the donor interview, the ACASI contained expanded content on demographics, sexual behavior, and other HIV risk factors questions. ResultsA total of 901 HIV-negative blood donors were interviewed. On the ACASI, 13% of donors (n=120) declared a risk factor that would have resulted in deferral that was not disclosed during the face-to-face assessment. The main risk factors identified were recent unprotected sex with an unknown or irregular partner (49 donors), sex with a person with exposure to blood or fluids (26 donors), multiple sexual partners (19 donors), and male-male sexual behavior (10 donors). Independent factors associated with the disclosure of any risk factor for HIV were age (40 years vs. 18-25 years; adjusted odds ratio [AOR],0.45; 95% confidence interval [CI], 0.23-0.88) and blood center (Hemope vs. Hemominas; AOR,2.51; 95% CI, 1.42-4.44). ConclusionACASI elicited increased disclosure of HIV risk factors among blood donors. ACASI may be a valuable modality of interview to be introduced in Brazilian blood banks.
conferenceObject PAST NEGATIVE EXPERIENCES WITH HIV TESTING PREDICTS TEST-SEEKING MOTIVATION AMONG BLOOD DONORS, SAO PAULO, BRAZIL(2013) GONCALEZ, T. T.; BLATYTA, P. B.; MONTEBELLO, S.; ESPOSTI, S. P. D. S.; HANGAI, F.; SALLES, N.; MENDRONE, A.; SABINO, E.; MCFARLAND, W.; SANTOS, F. M.- Analysis of donor deferral at three blood centers in Brazil(2013) GONCALEZ, Thelma T.; SABINO, Ester C.; SCHLUMPF, Karen S.; WRIGHT, David J.; MENDRONE, Alfredo; LOPES, Maria I.; LEAO, Silvana; MIRANDA, Carolina; CAPUANI, Ligia; CARNEIRO-PROIETTI, Anna Barbara F.; BASQUES, Fernando; FERREIRA, Joao E.; BUSCH, Michael; CUSTER, BrianBACKGROUND: The safety of the blood supply is ensured through several procedures from donor selection to testing of donated units. Examination of the donor deferrals at different centers provides insights into the role that deferrals play in transfusion safety. STUDY DESIGN AND METHODS: A cross-sectional descriptive study of prospective allogeneic blood donors at three large blood centers located in SAo Paulo, Belo Horizonte, and Recife, Brazil, from August 2007 to December 2009 was conducted. Deferrals were grouped into similar categories across the centers, and within each center frequencies out of all presentations were determined. RESULTS: Of 963,519 prospective blood donors at the three centers, 746,653 (77.5%) were accepted and 216,866 (22.5%) were deferred. Belo Horizonte had the highest overall deferral proportion of 27%, followed by Recife (23%) and SAo Paulo (19%). Females were more likely to be deferred than males (30% vs. 18%, respectively). The three most common deferral reasons were low hematocrit or hemoglobin, medical diagnoses, and higher-risk behavior. CONCLUSION: The types and frequencies of deferral vary substantially among the three blood centers. Factors that may explain the differences include demographic characteristics, the order in which health history and vital signs are taken, the staff training, and the way deferrals are coded by the centers among other policies. The results indicate that blood donor deferral in Brazil has regional aspects that should be considered when national policies are developed.
conferenceObject Previous Human Immunodeficiency (HIV) Testing in Blood Donors in Brazil(2013) BRUHN, R.; SABINO, E. C.; MORENO, E. C.; BLATYTA, P. F.; CARVALHO, S. M.; PROIETTI, A. C.; SAMPAIO, D.; LOPES, M.; CUSTER, B.; GONCALEZ, T. T.- Human immunodeficiency virus transfusion transmission despite nucleic acid testing(2013) SALLES, Nanci Alves; LEVI, Jose Eduardo; BARRETO, Claudia Cortese; SAMPAIO, Luciana Pereira; ROMANO, Camila Malta; SABINO, Ester Cerdeira; MENDRONE JUNIOR, Alfredo
- Antibody levels correlate with detection of Trypanosoma cruzi DNA by sensitive polymerase chain reaction assays in seropositive blood donors and possible resolution of infection over time(2013) SABINO, Ester C.; LEE, Tzong-Hae; MONTALVO, Lani; NGUYEN, Megan L.; LEIBY, David A.; CARRICK, Danielle M.; OTANI, Marcia M.; VINELLI, Elizabeth; WRIGHT, David; STRAMER, Susan L.; BUSCH, MichaelBACKGROUND: The clinical significance of anti-Trypanosomacruzi low-level reactive samples is incompletely understood. Polymerase chain reaction (PCR)-positive rates and antibody levels among seropositive blood donors in three countries are described. STUDY DESIGN AND METHODS: Follow-up samples were collected from T.cruzi-seropositive donors from 2008 through 2010 in the United States (n=195) and Honduras (n=58). Also 143 samples from Brazil in 1996 to 2002, originally positive by three serologic assays, were available and paired with contemporary follow-up samples from these donors. All samples were retested with Ortho enzyme-linked immunosorbent assay (ELISA). PCR assays were performed on coded sample panels by two laboratories (Blood Systems Research Institute [BSRI] and American Red Cross Holland Laboratory [ARC]) that amplified kinetoplast minicircle DNA sequences of T.cruzi. RESULTS: PCR testing at BSRI yielded slightly higher overall sensitivity and specificity (33 and 98%) compared with those at the ARC (28 and 94%). Among seropositive donors, PCR-positive rates varied by country (p<0.0001) for the BSRI laboratory: Brazil (57%), Honduras (32%), and the United States (14%). ELISA signal-to-cutoff ratios (S/CO) were significantly higher for PCR-positive compared to PCR-negative donors (p<0.05 for all comparisons). Additionally, PCR-negative Brazilian donors exhibited greater frequencies of antibody decline over time versus PCR-positive donors (p=0.003). CONCLUSION: For all three countries, persistent DNA positivity correlated with higher ELISA S/CO values, suggesting that high-level seroreactivity reflects chronic parasitemia. Significant S/CO declines in 10% of the PCR-negative Brazilian donors may indicate seroreversion after parasite clearance in the absence of treatment.