ESTER CERDEIRA SABINO

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Departamento de Patologia, Faculdade de Medicina - Docente
LIM/46 - Laboratório de Parasitologia Médica, Hospital das Clínicas, Faculdade de Medicina - Líder

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  • article 8 Citação(ões) na Scopus
    Anti-Trypanosoma cruzi Cross-Reactive Antibodies Detected at High Rate in Non-Exposed Individuals Living in Non-Endemic Regions: Seroprevalence and Association to Other Viral Serologies
    (2013) SABA, Esber S.; GUEYFFIER, Lucie; DICHTEL-DANJOY, Marie-Laure; POZZETTO, Bruno; BOURLET, Thomas; GUEYFFIER, Francois; MEKKI, Yahia; POTTEL, Hans; SABINO, Ester C.; VANHEMS, Philippe; ZREIN, Maan A.
    Cross-reactive antibodies are characterized by their recognition of antigens that are different from the trigger immunogen. This happens when the similarity between two different antigenic determinants becomes adequate enough to enable a specific binding with such cross-reactive antibodies. In the present manuscript, we report the presence, at an ""abnormal"" high frequency, of antibodies in blood samples from French human subjects cross-reacting with a synthetic-peptide antigen derived from a Trypanosoma cruzi (T. cruzi) protein sequence. As the vector of T. cruzi is virtually confined to South America, the parasite is unlikely to be the trigger immunogen of the cross-reactive antibodies detected in France. At present, the cross-reactive antibodies are measured by using an in-house ELISA method that employs the T. cruzi -peptide antigen. However, to underline their cross-reactive characteristics, we called these antibodies ""Trypanosoma cruzi Cross Reactive Antibodies"" or TcCRA. To validate their cross-reactive nature, these antibodies were affinity-purified from plasma of healthy blood donor and were then shown to specifically react with the T. cruzi parasite by immunofluorescence. Seroprevalence of TcCRA was estimated at 45% in serum samples of French blood donors while the same peptide-antigen reacts with about 96% of T. cruzi -infected Brazilian individuals. In addition, we compared the serology of TcCRA to other serologies such as HSV 1/2, EBV, HHV-6, CMV, VZV, adenovirus, parvovirus B19, mumps virus, rubella virus, respiratory syncytial virus, measles and enterovirus. No association was identified to any of the tested viruses. Furthermore, we tested sera from different age groups for TcCRA and found a progressive acquisition starting from early childhood. Our findings show a large seroprevalence of cross-reactive antibodies to a well-defined T. cruzi antigen and suggest they are induced by a widely spread immunogen, acquired from childhood. The etiology of TcCRA and their clinical relevance still need to be investigated.
  • conferenceObject
    DENGUE RNA AMONG BLOOD DONORS AND RECIPIENTS DURING LARGE EPIDEMICS OF DENV-4 IN RIO DE JANEIRO AND RECIFE, BRAZIL
    (2013) SABINO, E.; LOUREIRO, P.; LOPES, M.; CAPUANI, L.; OLIVEIRA, C.; OLIVEIRA, L.; LINNEN, J.; LEE, T-H; PRINCE, H.; MCCLURE, C.; CHOWDHURY, D.; GONCALEZ, T.; CUSTER, B.; BRAMBILLA, D.; BUSCH, M.
  • conferenceObject
    Motivation to Donate Among Granulocytes, Apheresis, and Whole Blood Donors in a Brazilian Blood Center
    (2013) ROCHA, P. C.; SABINO, E. C.; OLIVEIRA, C. D.; PATAVINO, G. M.; CAPUANI, L. D.; ALMEIDA-NETO, C. de
  • article 21 Citação(ões) na Scopus
    Investigation of human parvovirus B19 occurrence and genetic variability in different leukaemia entities
    (2013) COSTA, A. C. da; BENDIT, I.; OLIVEIRA, A. C. S. de; KALLAS, E. G.; SABINO, E. C.; SANABANI, S. S.
    Human parvovirus B19V (B19V) has been associated with various haematological disorders, but data on its prevalence in leukaemia are scarce. In this cross-sectional study, we investigated patients in Sao Paulo, Brazil with leukaemia to determine the molecular frequency of B19 variants and characterize the viral genetic variability by partial and complete sequencing of the coding of non-structural protein 1 (NS1)/viral capsid proteins 1 and 2 (VP1/VP2). The presence of B19V infections was investigated by PCR amplification of the viral NS1 gene fragment and confirmed by sequencing analysis. The NS1/VP1/VP2 and partially larger gene fragments of the NS1-positive samples were determined by overlapping nested PCR and direct sequencing results. The B19V NS1 was detected in 40 (16%) of 249 bone marrow samples including 12/78 (15.4%) acute lymphoblastic leukaemia, 25/155 (16.1%) acute myeloid leukaemia and 3/16 (18.7%) chronic myeloid leukaemia samples. Of the 40 participants, 25 (62.5%) were infected with genotype 1a and 15 (37.5%) with genotype 3b. The phylogenetic analysis of other regions revealed that 12/40 (30%) of the patients with leukaemia were co-infected with genotypes 1a and 3b. In addition, a new B19V intergenotypic recombinant (1a/3b) and an NS1 non-recombinant genotype 1a were detected in one patient. Our findings demonstrated a relatively high prevalence of B19V monoinfections and dual infections and provide, for the first time, evidence of inter-genotypic recombination in adults with leukaemia that may contribute to the genetic diversity of B19V and may also be a source of new emerging viral strains with future implications for diagnosis, therapy and efficient vaccine development.
  • article 50 Citação(ões) na Scopus
    Electrocardiographic Abnormalities in Trypanosoma cruzi Seropositive and Seronegative Former Blood Donors
    (2013) RIBEIRO, Antonio L.; SABINO, Ester C.; MARCOLINO, Milena S.; SALEMI, Vera M. C.; IANNI, Barbara M.; FERNANDES, Fabio; NASTARI, Luciano; ANTUNES, Andre; MENEZES, Marcia; OLIVEIRA, Claudia Di Lorenzo; SACHDEV, Vandana; CARRICK, Danielle M.; BUSCH, Michael P.; MURPHY, Eduard L.
    Background: Blood donor screening leads to large numbers of new diagnoses of Trypanosoma cruzi infection, with most donors in the asymptomatic chronic indeterminate form. Information on electrocardiogram (ECG) findings in infected blood donors is lacking and may help in counseling and recognizing those with more severe disease. Objectives: To assess the frequency of ECG abnormalities in T. cruzi seropositive relative to seronegative blood donors, and to recognize ECG abnormalities associated with left ventricular dysfunction. Methods: The study retrospectively enrolled 499 seropositive blood donors in Sao Paulo and Montes Claros, Brazil, and 483 seronegative control donors matched by site, gender, age, and year of blood donation. All subjects underwent a health clinical evaluation, ECG, and echocardiogram (Echo). ECG and Echo were reviewed blindly by centralized reading centers. Left ventricular (LV) dysfunction was defined as LV ejection fraction (EF), 0.50%. Results: Right bundle branch block and left anterior fascicular block, isolated or in association, were more frequently found in seropositive cases (p<0.0001). Both QRS and QTc duration were associated with LVEF values (correlation coefficients -0.159, p<0.0003, and -0.142, p = 0.002) and showed a moderate accuracy in the detection of reduced LVEF (area under the ROC curve: 0.778 and 0.790, both p<0.0001). Several ECG abnormalities were more commonly found in seropositive donors with depressed LVEF, including rhythm disorders (frequent supraventricular ectopic beats, atrial fibrillation or flutter and pacemaker), intraventricular blocks (right bundle branch block and left anterior fascicular block) and ischemic abnormalities (possible old myocardial infarction and major and minor ST abnormalities). ECG was sensitive (92%) for recognition of seropositive donors with depressed LVEF and had a high negative predictive value (99%) for ruling out LV dysfunction. Conclusions: ECG abnormalities are more frequent in seropositive than in seronegative blood donors. Several ECG abnormalities may help the recognition of seropositive cases with reduced LVEF who warrant careful follow-up and treatment.
  • article 9 Citação(ões) na Scopus
    Methodological guidelines for reducing the complexity of data warehouse development for transactional blood bank systems
    (2013) TAKECIAN, Pedro L.; OIKAWA, Marcio K.; BRAGHETTO, Kelly R.; ROCHA, Paulo; LUCENA, Fred; KAVOUNIS, Katherine; SCHLUMPF, Karen S.; ACKER, Susan; CARNEIRO-PROIETTI, Anna B. F.; SABINO, Ester C.; CUSTER, Brian; BUSCH, Michael P.; FERREIRA, Joao E.
    Over time, data warehouse (DW) systems have become more difficult to develop because of the growing heterogeneity of data sources. Despite advances in research and technology, DW projects are still too slow for pragmatic results to be generated. Here, we address the following question: how can the complexity of DW development for integration of heterogeneous transactional information systems be reduced? To answer this, we proposed methodological guidelines based on cycles of conceptual modeling and data analysis, to drive construction of a modular DW system. These guidelines were applied to the blood donation domain, successfully reducing the complexity of DW development.
  • article 17 Citação(ões) na Scopus
    Variability of HIV-1 Genomes among Children and Adolescents from Sao Paulo, Brazil
    (2013) SANABANI, Sabri Saeed; PESSOA, Rodrigo; OLIVEIRA, Ana Carolina Soares de; MARTINEZ, Vanessa Pouza; GIRET, Maria Teresa Maidana; SUCCI, Regina Celia de Menezes; CARVALHO, Karina; TOMIYAMA, Claudia Satiko; NIXON, Douglas F.; SABINO, Ester Cerdeira; KALLAS, Esper Georges
    Background: Genetic variability is a major feature of the human immunodeficiency virus type 1 (HIV-1) and considered the key factor to frustrating efforts to halt the virus epidemic. In this study, we aimed to investigate the genetic variability of HIV-1 strains among children and adolescents born from 1992 to 2009 in the state of Sao Paulo, Brazil. Methodology: Plasma and peripheral blood mononuclear cells (PBMC) were collected from 51 HIV-1-positive children and adolescents on ART followed between September 1992 and July 2009. After extraction, the genetic materials were used in a polymerase chain reaction (PCR) to amplify the viral near full length genomes (NFLGs) from 5 overlapped fragments. NFLGs and partial amplicons were directly sequenced and data were phylogenetically inferred. Results: Of the 51 samples studied, the NFLGs and partial fragments of HIV-1 from 42 PBMCs and 25 plasma were successfully subtyped. Results based on proviral DNA revealed that 22 (52.4%) patients were infected with subtype B, 16 (38.1%) were infected with BF1 mosaic variants and 4 (9.5%) were infected with sub-subtype F1. All the BF1 recombinants were unique and distinct from any previously identified unique or circulating recombinant forms in South America. Evidence of dual infections was detected in 3 patients coinfected with the same or distinct HIV-1 subtypes. Ten of the 31 (32.2%) and 12 of the 21 (57.1%) subjects with recovered proviral and plasma, respectively, protease sequences were infected with major mutants resistant to protease inhibitors. The V3 sequences of 14 patients with available sequences from PBMC/or plasma were predicted to be R5-tropic virus except for two patients who harbored an X4 strain. Conclusions: The high proportion of HIV-1 BF1 recombinant, coinfection rate and vertical transmission in Brazil merits urgent attention and effective measures to reduce the transmission of HIV among spouses and sex partners.
  • conferenceObject
    SELF-DISCLOSED TEST SEEKING AND HIV EXPOSURE RISK AMONG BLOOD DONORS IN BRAZIL
    (2013) BRUHN, R.; MORENO, E.; SABINO, E.; BLATYTA, P.; CARVALHO, S. Maia Farias de; CARNEIRO-PROIETTI, A. B.; SAMPAIO, D.; CUSTER, B.; GONCALEZ, T.
  • article 32 Citação(ões) na Scopus
    Prevalence of serologic markers for hepatitis B and C viruses in Brazilian blood donors and incidence and residual risk of transfusion transmission of hepatitis C virus
    (2013) ALMEIDA-NETO, Cesar de; SABINO, Ester Cerdeira; LIU, Jing; BLATYTA, Paula Fraiman; MENDRONE-JUNIOR, Alfredo; SALLES, Nanci Alves; LEAO, Silvana Carneiro; WRIGHT, David J.; BASQUES, Fernando Valadares; FERREIRA, Joao Eduardo; BUSCH, Michael P.; MURPHY, Edward L.
    BACKGROUND: We evaluate the current prevalence of serologic markers for hepatitisB virus (HBV) and hepatitisC virus (HCV) in blood donors and estimated HCV incidence and residual transfusion-transmitted risk at three large Brazilian blood centers. STUDY DESIGN AND METHODS: Data on whole blood and platelet donations were collected from January through December 2007, analyzed by center; donor type; age; sex; donation status; and serologic results for hepatitisB surface antigen (HBsAg), antibody to hepatitisB core antigen (anti-HBc), and anti-HCV. HBV and HCV prevalence rates were calculated for all first-time donations. HCV incidence was derived including interdonation intervals that preceded first repeat donations given during the study, and HCV residual risk was estimated for transfusions derived from repeat donors. RESULTS: There were 307,354 donations in 2007. Overall prevalence of concordant HBsAg and anti-HBc reactivity was 289 per 100,000 donations and of anti-HCV confirmed reactivity 191 per 100,000 donations. There were significant associations between older age and hepatitis markers, especially for HCV. HCV incidence was 3.11 (95% confidence interval, 0.77-7.03) per 100,000 person-years, and residual risk of HCV window-phase infections was estimated at 5.0 per million units transfused. CONCLUSION: Improvement in donor selection, socioeconomic conditions, and preventive measures, implemented over time, may have helped to decrease prevalence of HBV and HCV, relative to previous reports. Incidence and residual risk of HCV are also diminishing. Ongoing monitoring of HBV and HCV markers among Brazilian blood donors should help guide improved recruitment procedures, donor selection, laboratory screening, and counseling strategies.
  • article 28 Citação(ões) na Scopus
    Undisclosed human immunodeficiency virus risk factors identified through a computer-based questionnaire program among blood donors in Brazil
    (2013) BLATYTA, Paula Fraiman; CUSTER, Brian; GONCALEZ, Thelma Terezinha; BIRCH, Rebecca; LOPES, Maria Esther; FERREIRA, Maria Ines Lopes; PROIETTI, Anna Barbara Carneiro; SABINO, Ester Cerdeira; PAGE, Kimberly; ALMEIDA-NETO, Cesar de
    BackgroundHuman immunodeficiency virus (HIV) risk factor screening among blood donors remains a cornerstone for the safety of blood supply and is dependent on prospective donor self-disclosure and an attentive predonation interview. Audio computer-assisted structured interview (ACASI) has been shown to increase self-reporting of risk behaviors. Study Design and MethodsThis cross-sectional study was conducted between January 2009 and March 2011 at four Brazilian blood centers to identify the population of HIV-negative eligible blood donors that answered face-to-face interviews without disclosing risks, but subsequently disclosed deferrable risk factors by ACASI. Compared to the donor interview, the ACASI contained expanded content on demographics, sexual behavior, and other HIV risk factors questions. ResultsA total of 901 HIV-negative blood donors were interviewed. On the ACASI, 13% of donors (n=120) declared a risk factor that would have resulted in deferral that was not disclosed during the face-to-face assessment. The main risk factors identified were recent unprotected sex with an unknown or irregular partner (49 donors), sex with a person with exposure to blood or fluids (26 donors), multiple sexual partners (19 donors), and male-male sexual behavior (10 donors). Independent factors associated with the disclosure of any risk factor for HIV were age (40 years vs. 18-25 years; adjusted odds ratio [AOR],0.45; 95% confidence interval [CI], 0.23-0.88) and blood center (Hemope vs. Hemominas; AOR,2.51; 95% CI, 1.42-4.44). ConclusionACASI elicited increased disclosure of HIV risk factors among blood donors. ACASI may be a valuable modality of interview to be introduced in Brazilian blood banks.