LUIS HENRIQUE ISHIDA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
2 resultados
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- INTERNAL MAMMARY PERFORATOR VESSELS AS RECIPIENT SITE FOR MICROSURGICAL BREAST RECONSTRUCTION: A COMPARATIVE HISTOMORPHOMETRIC ANALYSIS AND INCIDENCE OF DEGENERATIVE VASCULAR CHANGES(2014) MUNHOZ, Alexandre Mendonca; ISHIDA, Luis H.; MONTAG, Eduardo; SAITO, Fabio L.; MENDES, Marcio; ALVES, Helio; GEMPERLI, RolfBackgroundIn microsurgical breast reconstruction, an adequate selection of recipient vessels is crucial for a successful outcome. Although the internal mammary (IM) vessels offer an attractive option, the internal mammary perforator (IMP) vessels are becoming a reliable alternative. The purpose of this study is to investigate the external diameters, lumen area, and atherosclerotic lesions changes of the IMP, IM, and deep inferior epigastric (DIE) vessels through quantitative and qualitative histomorphometric analysis. MethodsNinety-six vessels of bilateral IM, IMP, and DIE vessels from 16 fresh female cadavers were evaluated. Mean age was 54.065.7 years. External diameters, lumen area, and degenerative changes of the tunica intimae and media were analyzed by qualitative histomorphometric analysis. ResultsSeventy-one vessels (20 IM, 31 IMP, and 20 DIE vessels) were included in the final histological analysis. A statistically lower external diameters and lumen area were presented by the IMP. The DIE vessels showed a lower incidence (10%) of moderate and severe intimal layer degenerative changes (P=0.0589). The IMP and DIE vessels showed a lower incidence (9.4 and 25%, respectively) of major media layer degenerative changes (P=0.0001). No major arterial degenerative lesions were observed in the IMP arteries. ConclusionAlthough the IMP external diameters and lumen area were lower than the IM, the results of this study indicated that the tunica media layer in the IMP is less damaged than the other recipient vessels. The results of the comparative histological study permitted to describe additional advantages and disadvantages of using IMP as a recipient vessel for free flap breast reconstruction. (c) 2013 Wiley Periodicals, Inc. Microsurgery 34:217-223, 2014.
- Homozygous truncating PTPRF mutation causes athelia(2014) BORCK, Guntram; VRIES, Liat de; WU, Hsin-Jung; SMIRIN-YOSEF, Pola; NUERNBERG, Gudrun; LAGOVSKY, Irina; ISHIDA, Luis Henrique; THIERRY, Patrick; WIECZOREK, Dagmar; NUERNBERG, Peter; FOLEY, John; KUBISCH, Christian; BASEL-VANAGAITE, LinaAthelia is a very rare entity that is defined by the absence of the nipple-areola complex. It can affect either sex and is mostly part of syndromes including other congenital or ectodermal anomalies, such as limb-mammary syndrome, scalp-ear-nipple syndrome, or ectodermal dysplasias. Here, we report on three children from two branches of an extended consanguineous Israeli Arab family, a girl and two boys, who presented with a spectrum of nipple anomalies ranging from unilateral hypothelia to bilateral athelia but no other consistently associated anomalies except a characteristic eyebrow shape. Using homozygosity mapping after single nucleotide polymorphism (SNP) array genotyping and candidate gene sequencing we identified a homozygous frameshift mutation in PTPRF as the likely cause of nipple anomalies in this family. PTPRF encodes a receptor-type protein phosphatase that localizes to adherens junctions and may be involved in the regulation of epithelial cell-cell contacts, peptide growth factor signaling, and the canonical Wnt pathway. Together with previous reports on female mutant Ptprf mice, which have a lactation defect, and disruption of one allele of PTPRF by a balanced translocation in a woman with amastia, our results indicate a key role for PTPRF in the development of the nipple-areola region.