LUIZ FERNANDO PORTUGAL FUCHS
Projetos de Pesquisa
Unidades Organizacionais
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
5 resultados
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- Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation(2015) LIMA, Gisele Negro; MAGANHIN, Carla Cristina; SIMOES, Ricardo Santos; BARACAT, Maria Candida Pinheiro; SASSO, Gisela Rodrigues da Silva; FUCHS, Luiz Fernando Portugal; SIMOES, Manuel de Jesus; BARACAT, Edmund Chada; SOARES JUNIOR, Jose MariaOBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 mu g/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip (R) Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip (R) Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis.
conferenceObject Imunoexpression of BCL-2 and BAX in ovarian follicles of pinealectomized or melatonin-treated pinealectomized rats(2015) FUCHS, Luiz F.; MAGANHIN, Carla C.; SASSO, Gisela R.; SIMOES, Ricardo S.; BARACAT, Edmund C.; SOARES- JR., Jose MariaconferenceObject Expression and influence ovarian function by the upregulation and downregulation of steroidogenesis related genes in ovary of pinealectomized rats in proestrous phase: Folistatin and Inhibin-beta A(2015) MAGANHIN, Carla C.; FUCHS, Luiz F.; SIMOES, Ricardo S.; SASSO, Gisela R.; BARACAT, Edmund C.; SOARES- JR., Jose Maria- Effects of metformin on the adrenal cortex of androgenized rats(2015) MACEDO, Lilian Alves; CARBONEL, Adriana Aparecida Ferraz; SIMOES, Ricardo Santos; FUCHS, Luiz Fernando Portugal; AMARAL, Vinicius Cestari do; SIMONCINI, Tommaso; SIMOES, Manuel Jesus Santos; BARACAT, Edmund Chada; SOARES JR., Jose MariaObjective: To evaluate the sex steroid profile and histomorphometry of the adrenal cortical zones of androgenized rats (wistar) with polycystic ovary syndrome treated with metformin. Study design: Thirty animals were divided into three groups: GC (regular estrous cycle), GPE (permanent estrus), and GPEM (permanent estrus + metformin 28 mg/kg for 50 days). At the end of this period, blood was collected for hormone measurement. The width of the adrenal cortical zones and the nuclear volumes were analyzed by histomorphometry. The ANOVA test was used in the statistical analysis. Results: The adrenal glands of the androgenized animals were larger and more intensely vascularized than those of the other groups. The concentration of androstenedione in GPE was higher than that in the other groups (0.4 +/- 0.1*>= 0.2 +/- 0.1 = 0.2 +/- 01, *p<50.05). The width of the zona glomerulosa and of the zona reticularis and their nuclear volumes were greater in GPE compared to those of the other groups (GPE*>GPEM = GC, *p<50.05). Conclusion: Metformin treatment may decrease the serum levels of androstenedione as well as the width and the nuclear volumes of the zona glomerulosa and of the zona reticularis in androgenized animals.
conferenceObject Imnunoexpression of estrogen receptors may decrease produce melatonin in ovaries of pinealectomized female rats(2015) MAGANHIN, Carla C.; FUCHS, Luiz F.; SASSO, Gisela R.; SIMOES, Ricardo S.; BARACAT, Edmund C.; SOARES JUNIOR, Jose M.