MARIANA MATERA VERAS
Projetos de Pesquisa
Unidades Organizacionais
LIM/05 - Laboratório de Poluição Atmosférica Experimental, Hospital das Clínicas, Faculdade de Medicina - Líder
73 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 73
- Periurethral muscle-derived mononuclear cell injection improves urethral sphincter restoration in rats(2017) TURCO, Marcelo Pitelli; SOUZA, Alex Balduino de; SOUSA, Isida de Campos; FRATINI, Paula; VERAS, Mariana Matera; RODRIGUES, Marcio Nogueira; BESSA JR., Jose de; BROLIO, Marina Pandolphi; LEITE, Katia Ramos Moreira; BRUSCHINI, Homero; SROUGI, Miguel; MIGLINO, Maria Angelica; GOMES, Cristiano MendesAimsInvestigate the effect of a novel cell-based therapy with skeletal muscle-derived mononuclear cells (SMDMCs) in a rat model of stress urinary incontinence. MethodsMale Wistar-Kyoto rats' hind limb muscles were enzymatically dissociated, and SMDMCs were isolated without needing expansion. The cell population was characterized. Twenty female rats underwent urethrolysis. One week later, 10 rats received periurethral injection of 10(6) cells (SMDMC group), and 10 rats received saline injections (Saline group). Ten rats underwent sham surgery (Sham group). Four weeks after injection, animals were euthanized and the urethra was removed. The incorporation of SMDMCs in the female urethra was evaluated with fluorescence in situ hybridization for the detection of Y-chromosomes. Hematoxylin and eosin, Masson's trichrome staining, and immunohistochemistry for actin and myosin were performed. The muscle/connective tissue, actin and myosin ratios were calculated. Morphological evaluation of the urethral diameters and fractional areas of the lumen, mucosa, and muscular layer was performed. ResultsSMDMCs population was consistent with the presence of muscle cells, muscle satellite cells, perivascular cells, muscle progenitor cells, and endothelial cells. SMDMCs were incorporated into the urethra. A significant decrease in the muscle/connective tissue ratio was observed in the Saline group compared with the SMDMC and Sham groups. The proportions of actin and myosin were significantly decreased in the Saline group. No differences were observed in the morphometric parameters. ConclusionsSDMSC were incorporated into the rat urethra and promoted histological recovery of the damaged urethral sphincter, resulting in decreased connective tissue deposition and increased muscle content.
- Transcriptomic analysis of purified human cortical microglia reveals age-associated changes(2017) GALATRO, Thais F.; HOLTMAN, Inge R.; LERARIO, Antonio M.; VAINCHTEIN, Ilia D.; BROUWER, Nieske; SOLA, Paula R.; VERAS, Mariana M.; PEREIRA, Tulio F.; LEITE, Renata E. P.; MOLLER, Thomas; WES, Paul D.; SOGAYAR, Mari C.; LAMAN, Jon D.; DUNNEN, Wilfred den; PASQUALUCCI, Carlos A.; OBA-SHINJO, Sueli M.; BODDEKE, Erik W. G. M.; MARIE, Suely K. N.; EGGEN, Bart J. L.Microglia are essential for CNS homeostasis and innate neuroimmune function, and play important roles in neurodegeneration and brain aging. Here we present gene expression profiles of purified microglia isolated at autopsy from the parietal cortex of 39 human subjects with intact cognition. Overall, genes expressed by human microglia were similar to those in mouse, including established microglial genes CX3CR1, P2RY12 and ITGAM (CD11B). However, a number of immune genes, not identified as part of the mouse microglial signature, were abundantly expressed in human microglia, including TLR, F-c gamma and SIGLEC receptors, as well as TAL1 and IFI16, regulators of proliferation and cell cycle. Age-associated changes in human microglia were enriched for genes involved in cell adhesion, axonal guidance, cell surface receptor expression and actin (dis)assembly. Limited overlap was observed in microglial genes regulated during aging between mice and humans, indicating that human and mouse microglia age differently.
conferenceObject Fine particulate matter in the inspired air within the limits recommended by WHO alters placental structure(2014) SOTO, Sonia; LOPES, Karen; VERAS, Mariana; HEIMANN, JoelconferenceObject Perinatal exposure to PM2.5 can cause long-lasting changes in neurotransmission and neuroinflammation, and predispose to epileptic seizure(2021) FERNANDES, Maria Jose Silva; ARAUJO, Luis Fernando Sierra de; PEROSA, Regina; VERAS, Mariana Matera; CARLETTI, Carla OliveiraconferenceObject IMPACTS OF RECREATIONAL USE OF CANNABIS SATIVA ON GESTATIONAL AND DEVELOPMENTAL OUTCOMES IN MICE: PRELIMINARY RESULTS(2015) BENEVENUTO, S. G. M.; BELOTTI, L.; COSTA, N. S. X.; DOLHNIKOFF, M.; RIBEIRO JUNIOR, G.; LOURENCO, D. T.; VERAS, M. M.- Inhaled ultrafine particles, epigenetics and systemic autoimmune rheumatic diseases(2020) FARHAT, Sylvia Costa Lima; YARIWAKE, Victor Yuji; VERAS, Mariana Matera; BRAGA, Alfesio Luis Ferreira; MALUF, Adriana Elias; SILVA, Clovis Artur
- A Short Period of Exposure to Air Pollution (PM2.5) Is Sufficient to Induce Dysbiosis, Hyperphagia, and Fat Mass Gain(2019) ZORDAO, Olivia P.; SANTOS, Andrey; CAMPOLIM, Clara Machado; SR., Raquel Ataide Lima; FERREIRA, Clilton Krauss; SAAD, Mario; SALDIVA, Paulo; VERAS, Mariana; PRADA, Patricia O.
conferenceObject Chronicle air pollution exposition and semen quality in hypercolesterolemic mice(2013) PERES, M. A.; CASTRO, K.; RIBEIRO-JUNIOR, G.; MENDES, C.; LOSANO, J.; VERAS, M.; SALDIVA, P.Air pollution (AP) is associated to lose of semen quality. It is also known that some population groups are more susceptible to atmospheric pollution effects due to a pre-existing health condition. This work detailed the effects AP in semen quality of mice knockout for low density lipoprotein receptor that represents an important risk group of human population. Animals were exposed to AP during pre and/or post natal period until adult life, with or without hypercholesterolemic diet. Semen analysis comprised sperm number and morphology, membrane, acrosome and DNA integrity, mitochondrial activity, oxidative stress and cytokines expression of seminal vesicle. The combination of the three factors (AP pre/post natal exposure and hypercholesterolemia) negatively impacted DNA fragmentation, mitochondrial activity, membrane integrity and oxidative stress. Other parameters did not show significant differences. Air pollution combined with hypercholesterolemia condition potentiated the negative effects on important parameters of semen leading male mice to present low fertility/infertility.conferenceObject Influence of exposure to concentrated urban air particles on the respiratory system of (LDLr-/-) mice(2014) MAUAD, Thais; ALEMANY, Adair; VERAS, Mariana; JUNIOR, Gabriel Ribeiro; COSTA, Natalia; CATANOZI, Sergio; ABDALLA, Dulcineia; SALDIVA, PauloconferenceObject Human microglia transcriptome and cross-species analysis(2015) GALATRO, T. F. de A.; HOLTMAN, I. R.; BROUWER, N.; SOLA, P.; REIS, G. N.; VAINCHTEIN, I. D.; VERAS, M.; PEREIRA, T.; PASQUALUCCI, C.; SOGAYAR, M. C.; BODDEKE, E. W. G.; MARIE, S. K. N.; EGGEN, B. J. L.