PAULO ROBERTO DOS SANTOS SILVA
Projetos de Pesquisa
Unidades Organizacionais
Instituto de Ortopedia e Traumatologia, Hospital das Clínicas, Faculdade de Medicina
LIM/41 - Laboratório de Investigação Médica do Sistema Músculoesquelético, Hospital das Clínicas, Faculdade de Medicina
LIM/41 - Laboratório de Investigação Médica do Sistema Músculoesquelético, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
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- Moderate levels of physical fitness maintain telomere length in non-senescent T CD8(+) cells of aged men(2020) BASTOS, Marta Ferreira; MATIAS, Manuella de Sousa Toledo; ALONSO, Angelica Castilho; SILVA, Leia Cristina Rodrigues; ARAUJO, Adriana Ladeira de; SILVA, Paulo Roberto; BENARD, Gil; BOCALINI, Danilo Sales; BAKER, Julien Steven; LEME, Luiz Eugenio GarcezOBJECTIVES: Immunosenescence is an age-associated change characterized by a decreased immune response. Although physical activity has been described as fundamental for maintaining the quality of life, few studies have evaluated the effects of different levels of exercise on telomere length in aged populations. The present study aimed to analyze the effects of different levels of physical activity, classified by the Maximal oxygen consumption (VO2 max) values, on the telomere length of memory Cluster of differentiation (CD) CD4(+) (CD45RO(neg) and CD45RO(+) ), effector CD8(+)CD28(neg), and CD8(+) CD28(+) T cells in aged individuals. METHODS: Fifty-three healthy elderly men (aged 65-85 years) were included in this study. Their fitness level was classified according to the American College of Sports Medicine (ACSM) for VO2 max (mL/kg/min). Blood samples were obtained from all participants to analyze the percentage of CD3, CD4, CD8, CD28(+), naive, and subpopulations of memory T cells by using flow cytometry. Furthermore, using the Flow-FISH methodology, the CD4(+) CD45RO(+), CD4(+) CD45RO(neg), CD8(+) CD28(+), and CD8(+) CD28(neg)T cell telomere lengths were measured. RESULTS: There was a greater proportion of effector memory T CD4(+) cells and longer telomeres in CD8(+) CD28(+) T cells in the moderate physical fitness group than in the other groups. There was a higher proportion of terminally differentiated memory effector T cells in the low physical fitness group. CONCLUSION: A moderate physical activity may positively influence the telomere shortening of CD28(+) CD8(+)1 cells. However, additional studies are necessary to evaluate the importance of this finding with regard to immune function responses in older men.
- Physical and pulmonary capacities of individuals with severe coronavirus disease after hospital discharge: A preliminary cross-sectional study based on cluster analysis(2021) ALONSO, Angelica Castilho; SILVA-SANTOS, Paulo Roberto; QUINTANA, Marilia Simoes Lopes; SILVA, Vanderlei Carneiro da; BRECH, Guilherme Carlos; BARBOSA, Lorena Goncalves; POMPEU, Jose Eduardo; SILVA, Erika Christina Gouveia e; SILVA, Elizabeth Mendes da; GODOY, Caroline Gil de; GREVE, Julia Maria D'AndreaOBJECTIVE: This study aimed to analyze the physical and pulmonary capacities of hospitalized patients with severe coronavirus disease and its correlation with the time of hospitalization and complications involved. METHODS: A total of 54 patients, aged >= 18 years of both sexes, were evaluated 2-4 months after hospital discharge in Sao Paulo, Brazil. The physical characteristics analyzed were muscle strength, balance, flexibility, and pulmonary function. The K-means cluster algorithm was used to identify patients with similar physical and pulmonary capacities, related to the time of hospitalization. RESULTS: Two clusters were derived using the K-means algorithm. Patients allocated in cluster 1 had fewer days of hospitalization, intensive care, and intubation than those in cluster 2, which reflected a better physical performance, strength, balance, and pulmonary condition, even 2-4 months after discharge. Days of hospitalization were inversely related to muscle strength, physical performance, and lung function: hand grip D (r= -0.28, p=0.04), Short Physical Performance Battery score (r= -0.28, p=0.03), and forced vital capacity (r= -0.29, p=0.03). CONCLUSION: Patients with a longer hospitalization time and complications progressed with greater loss of physical and pulmonary capacities.
- BONE MINERAL DENSITY AND BODY COMPOSITION IN ELDERLY RUNNERS: SIX-YEAR FOLLOW-UP(2019) ALONSO, Angelica Castilho; ERNANDES, Rita de Cassia; PEREIRA, Regina Helena Marques; BECKER, Ricardo Andre; MACHADO-LIMA, Adriana; SILVA-SANTOS, Paulo Roberto; GREVE, Julia Maria D'Andrea; GARCEZ-LEME, Luiz EugenioObjective: To evaluate bone mineral density (BMD) and body composition over a six-year period in elderly long-distance runners. Methods: We analyzed the medical records of elderly athletes who were long-distance runners, were participants of the IOT-HCFMUSP Orthogeriatric Group, and had their BMD evaluated between 2001 and 2007; of these athletes, 11 were included in the study. Inclusion criteria: athletes should be long-distance runners, should not stop long-distance running during the six-year period, and should have undergone BMD and body composition evaluations. Body composition was evaluated using bone densitometry with dual-energy X-ray absorptiometry with a Lunar-DPX device. Results: Over the six-year period, body composition remained stable, but there was a significant increase only in the fat percentage (p = 0.003). Conclusion: Long-distance running may maintain BMD but may lead to an increase in the fat percentage in elderly runners.