PAULO ROBERTO DOS SANTOS SILVA
Projetos de Pesquisa
Unidades Organizacionais
Instituto de Ortopedia e Traumatologia, Hospital das Clínicas, Faculdade de Medicina
LIM/41 - Laboratório de Investigação Médica do Sistema Músculoesquelético, Hospital das Clínicas, Faculdade de Medicina
LIM/41 - Laboratório de Investigação Médica do Sistema Músculoesquelético, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
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- Moderate levels of physical fitness maintain telomere length in non-senescent T CD8(+) cells of aged men(2020) BASTOS, Marta Ferreira; MATIAS, Manuella de Sousa Toledo; ALONSO, Angelica Castilho; SILVA, Leia Cristina Rodrigues; ARAUJO, Adriana Ladeira de; SILVA, Paulo Roberto; BENARD, Gil; BOCALINI, Danilo Sales; BAKER, Julien Steven; LEME, Luiz Eugenio GarcezOBJECTIVES: Immunosenescence is an age-associated change characterized by a decreased immune response. Although physical activity has been described as fundamental for maintaining the quality of life, few studies have evaluated the effects of different levels of exercise on telomere length in aged populations. The present study aimed to analyze the effects of different levels of physical activity, classified by the Maximal oxygen consumption (VO2 max) values, on the telomere length of memory Cluster of differentiation (CD) CD4(+) (CD45RO(neg) and CD45RO(+) ), effector CD8(+)CD28(neg), and CD8(+) CD28(+) T cells in aged individuals. METHODS: Fifty-three healthy elderly men (aged 65-85 years) were included in this study. Their fitness level was classified according to the American College of Sports Medicine (ACSM) for VO2 max (mL/kg/min). Blood samples were obtained from all participants to analyze the percentage of CD3, CD4, CD8, CD28(+), naive, and subpopulations of memory T cells by using flow cytometry. Furthermore, using the Flow-FISH methodology, the CD4(+) CD45RO(+), CD4(+) CD45RO(neg), CD8(+) CD28(+), and CD8(+) CD28(neg)T cell telomere lengths were measured. RESULTS: There was a greater proportion of effector memory T CD4(+) cells and longer telomeres in CD8(+) CD28(+) T cells in the moderate physical fitness group than in the other groups. There was a higher proportion of terminally differentiated memory effector T cells in the low physical fitness group. CONCLUSION: A moderate physical activity may positively influence the telomere shortening of CD28(+) CD8(+)1 cells. However, additional studies are necessary to evaluate the importance of this finding with regard to immune function responses in older men.
- Muscle strength and body composition in severe obesity(2017) GADDUCCI, Alexandre Vieira; CLEVA, Roberto de; SANTAREM, Gabriela Correia de Faria; SILVA, Paulo Roberto Santos; GREVE, Julia Maria D'Andrea; SANTO, Marco AurelioOBJECTIVE: The aim of our study was to evaluate associations between maximum voluntary contraction torques of the lower limbs and body composition for subjects with severe obesity. METHODS: Body composition was evaluated by bioelectrical impedance analysis, and maximum voluntary contraction torques of the lower limbs were measured using an isokinetic dynamometer. One hundred thirty-two patients were enrolled (100 females and 32 males). Eighty-seven patients had a body mass index between 40 and 49.9 kg/m(2) (the A group), and 45 patients had a body mass index between 50 and 59.9 kg/m(2) (the B group). RESULTS: Absolute extension and flexion torques had weak associations with fat-free mass but a moderate association with absolute extension torque and fat-free mass of the lower limbs. There were no significant differences between the A and B groups with respect to absolute extension and flexion torques. For the A group, absolute extension and flexion torques were moderately associated with fat-free mass and with fat-free mass of the lower limbs. For the B group, there were only moderate associations between absolute extension and flexion torques with fat-free mass of the lower limbs. CONCLUSIONS: Our findings demonstrate that both groups exhibited similar absolute torque values. There were weak to moderate associations between absolute extension and flexion torques and fat-free mass but a moderate association with fat-free mass of the lower limbs. Individuals with severe obesity should strive for greater absolute torques, fat-free mass and especially fat-free mass of the lower limbs to prevent functional limitations and physical incapacity.
- Association between muscle strength and the cardiopulmonary status of individuals living with HIV/AIDS(2013) RASO, Vagner; SHEPHARD, Roy J.; CASSEB, Jorge; DUARTE, Alberto Jose da Silva; SILVA, Paulo Roberto Santos; GREVE, Julia Maria D'AndreaOBJECTIVE: The purpose of this study was to compare aerobic function [ anaerobic threshold (%(V) over dotO(2)-AT), respiratory compensation point (%(V) over dotO(2)-RCP) and peak oxygen uptake ((V) over dotO(2peak))] between physically active patients with HIV/AIDS and matched controls and to examine associations between disease status, poor muscle strength, depression (as estimated by the profile of mood states questionnaire) and the aerobic performance of patients. METHODS: Progressive treadmill test data for %(V) over dotO(2)-AT (V-slope method), RCP and ((V) over dotO(2peak)) were compared between 39 male patients with HIV/AIDS (age 40.6 +/- 1.4 years) and 28 male controls (age 44.4 +/- 2.1 years) drawn from the same community and matched for habitual physical activity. Within-patient data were also examined in relation to CD4(+) counts (nadir and current data) and peak isokinetic knee torque. RESULTS: AT, RCP and ((V) over dotO(2peak)) values were generally similar for patients and controls. Within the patient sample, binary classification suggested that AT, RCP and ((V) over dotO(2peak)) values were not associated with either the nadir or current CD4(+) count, but treadmill test variables were positively associated with peak isokinetic knee torque. CONCLUSION: The aerobic performance of physically active patients with HIV/AIDS is generally well conserved. Nevertheless, poor muscle strength is observed in some HIV/AIDS patients, which is associated with lower anaerobic power and ((V) over dotO(2peak)), suggesting the possibility of enhancing the aerobic performance of patients with weak muscles through appropriate muscle-strengthening activities.
- Contemporary reflection on the educational levels of high-performance soccer players in Brazil(2023) SANTOS-SILVA, Paulo Roberto; GREVE, Julia Maria D'Andrea; SILVA, Renato Luis da; SPINOLA, Marcelo MesquitaObjective: As the soccer culture in Brazil is more popular than schooling, this study reflected on the formal education levels of soccer (football) players through descriptive and quantitative analyses. Methods: We evaluated 179 national soccer players playing various positions on different teams across six seasons (2012, 2013, 2014, 2015, 2016, and 2022). Data were collected using a questionnaire comprising the following variables: age, position in the pitch, age of professionalization, and education (years of study). The data were distributed according to the pitch position. Results: The mean age, length of professional career, and professionalization age were 23 +/- 6 years, 7 +/- 5 years, and 17 +/- 2 years, respectively. In terms of education, 121 athletes (67%) completed high school, equivalent to 11 years of study. Only 5.5% completed higher education, with defensive players (goalkeepers and defenders) being the most educated at 37% (66/179). According to 2017 figures from the Pesquisa Nacional por Amostra de Domicilios Continua of the Federal Government of Brazil, the schooling rate is 31.7%, wherein 46.1% of Brazilians aged 25 years or over have completed education. In this study of 179 athletes, 67% had completed high school. Thus, players outrank the Brazilian population in terms of achieving a high school education. There were marked disparities in the relative proportions of goalkeepers (85%), defensive players (68%), midfielders (63%), and forward players (64%) in terms of their education. Conclusion: Our survey revealed that attending higher education remains a distant reality in Brazil.
- Post-analysis methods for lactate threshold depend on training intensity and aerobic capacity in runners. An experimental laboratory study(2016) FERNANDES, Tiago Lazzaretti; NUNES, Romulo dos Santos Sobreira; ABAD, Cesar Cavinato Cal; SILVA, Andrea Clemente Baptista; SOUZA, Larissa Silva; SILVA, Paulo Roberto Santos; ALBUQUERQUE, Cyro; IRIGOYEN, Maria Claudia; HERNANDEZ, Arnaldo JoseCONTEXT AND OBJECTIVE: This study aimed to evaluate different mathematical post-analysis methods of determining lactate threshold in highly and lowly trained endurance runners. DESIGN AND SETTING: Experimental laboratory study, in a tertiary-level public university hospital. METHOD: Twenty-seven male endurance runners were divided into two training load groups: lowly trained (frequency < 4 times per week, < 6 consecutive months, training velocity >= 5.0 min/km) and highly trained (frequency >= 4 times per week, >= 6 consecutive months, training velocity < 5.0 min/km). The subjects performed an incremental treadmill protocol, with 1 km/h increases at each subsequent 4-minute stage. Fingerprint blood-lactate analysis was performed at the end of each stage. The lactate threshold (i.e. the running velocity at which blood lactate levels began to exponentially increase) was measured using three different methods: increase in blood lactate of 1 mmol/l at stages (DT1), absolute 4 mmol/l blood lactate concentration (4 mmol), and the semi-log method (semi-log). ANOVA was used to compare different lactate threshold methods and training groups. RESULTS: Highly trained athletes showed significantly greater lactate thresholds than lowly trained runners, regardless of the calculation method used. When all the subject data were combined, DT1 and semi-log were not different, while 4 mmol was significantly lower than the other two methods. These same trends were observed when comparing lactate threshold methods in the lowly trained group. However, 4 mmol was only significantly lower than DT1 in the highly trained group. CONCLUSION: The 4 mmol protocol did not show lactate threshold measurements comparable with DT1 and semi-log protocols among lowly trained athletes.
- Effect of concurrent training in unilateral transtibial amputees using Paralympic athletes as a control group(2023) GRECCO, Marcus Vinicius; BRECH, Guilherme Carlos; SOARES-JUNIOR, Jose Maria; BARACAT, Edmund Chada; GREVE, Julia Maria D'Andrea; SILVA, Paulo Roberto SantosContext: Transtibial Amputation (TA) predisposes to a sedentary lifestyle.Objectives: To evaluate the efficiency of a short-term (8-week) Concurrent Training (CT) program in Unilateral Transtibial Amputees (UTA) and to compare it with the physical condition of a group of Paralympic athletes in preparation for the Rio de Janeiro Paralympics.Design: This was a longitudinal, prospective and controlled trial study.Methods: Thirty-four male subjects with UTA and using prostheses for six months or more were selected for this study. They were divided into two groups: Group 1 (G1) -17 non-athlete and untrained UTA and Group 2 (G2) -17 paralympic athletes with active UTA in the training phase. G1 was evaluated before and after eight weeks of CT and G2 made a single evaluation for control. All were submitted to anamnesis, clinical evaluation (blood pres-sure, electrocardiogram, and heart rate) and cardiopulmonary exercise testing on a lower limb cycle ergometer, and isokinetic knee dynamometry. The CT of G1 included resistance exercise and aerobic interval training on a stationary bicycle and G2 followed the training of the Paralympic teams.Results: Patients were retested by the same methods after CT. The two most important central dependent variables (maximal oxygen uptake and muscular strength) increased by 22% and knee extensor and flexor strength by 106% and 97%, respectively.Conclusion: After eight weeks of CT, there was an improvement in general functional condition, muscle strength, and cardiorespiratory performance improving protection against chronic diseases and quality of life.
- Physical and pulmonary capacities of individuals with severe coronavirus disease after hospital discharge: A preliminary cross-sectional study based on cluster analysis(2021) ALONSO, Angelica Castilho; SILVA-SANTOS, Paulo Roberto; QUINTANA, Marilia Simoes Lopes; SILVA, Vanderlei Carneiro da; BRECH, Guilherme Carlos; BARBOSA, Lorena Goncalves; POMPEU, Jose Eduardo; SILVA, Erika Christina Gouveia e; SILVA, Elizabeth Mendes da; GODOY, Caroline Gil de; GREVE, Julia Maria D'AndreaOBJECTIVE: This study aimed to analyze the physical and pulmonary capacities of hospitalized patients with severe coronavirus disease and its correlation with the time of hospitalization and complications involved. METHODS: A total of 54 patients, aged >= 18 years of both sexes, were evaluated 2-4 months after hospital discharge in Sao Paulo, Brazil. The physical characteristics analyzed were muscle strength, balance, flexibility, and pulmonary function. The K-means cluster algorithm was used to identify patients with similar physical and pulmonary capacities, related to the time of hospitalization. RESULTS: Two clusters were derived using the K-means algorithm. Patients allocated in cluster 1 had fewer days of hospitalization, intensive care, and intubation than those in cluster 2, which reflected a better physical performance, strength, balance, and pulmonary condition, even 2-4 months after discharge. Days of hospitalization were inversely related to muscle strength, physical performance, and lung function: hand grip D (r= -0.28, p=0.04), Short Physical Performance Battery score (r= -0.28, p=0.03), and forced vital capacity (r= -0.29, p=0.03). CONCLUSION: Patients with a longer hospitalization time and complications progressed with greater loss of physical and pulmonary capacities.
- Effect of 12-week rehearsal on cardiorespiratory fitness and body composition in Brazilian samba dancers(2023) DUARTE, Cicera Claudinea; SANTOS-SILVA, Paulo Roberto; PALUDO, Ana Carolina; GRECCO, Marcus Vinicius; GREVE, Julia Maria D'AndreaObjective: To investigate the effect of 12 weeks of rehearsals on cardiorespiratory parameters and body composition in Brazilian samba dancers belonging to a first-league samba school.Methods: Twenty-six women were divided into a Samba Group (n=13) and a Control Group (n=13). Cardiorespiratory parameters (cardiopulmonary exercise test) and body composition (skinfold assessment) were assessed before and after the 12 weeks of rehearsals. The Samba Group rehearsed three times per week for 30-60 minutes, and the Control Group participated in no physical activity. A comparison test was performed within and between groups, with p<0.05 indicating statistical significance.Results: Compared with the Control Group, the Samba Group showed a significant increase in maximal oxygen uptake (19%), oxygen pulse (13%), and lean body mass (3%) and a decrease in body fat percentage (11%) and fat mass (12%).Conclusion: Twelve weeks of samba dance rehearsals improved the cardiorespiratory and body composition parameters in women dancers compared with the Control Group. These findings suggest that dancing samba regularly can increase physical activity levels and positively affect the health parameters of samba dancers.