ARISTIDES TADEU CORREIA
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/61 - Laboratório de Pesquisa em Cirurgia Torácica, Hospital das Clínicas, Faculdade de Medicina
LIM/61 - Laboratório de Pesquisa em Cirurgia Torácica, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
Agora exibindo 1 - 2 de 2
- Modulation of Alveolar Macrophage Activity by Eugenol Attenuates Cigarette-Smoke-Induced Acute Lung Injury in Mice(2023) BARBOSA-DE-OLIVEIRA, Maria Clara; OLIVEIRA-MELO, Paolo; SILVA, Marcos Henrique Goncalves da; SILVA, Flavio Santos da; SILVA, Felipe Andrade Carvalho da; ARAUJO, Bruno Vinicios Silva de; OLIVEIRA, Moacir Franco de; CORREIA, Aristides Tadeu; SAKAMOTO, Sidnei Miyoshi; VALENCA, Samuel Santos; LANZETTI, Manuella; SCHMIDT, Martina; KENNEDY-FEITOSA, EmanuelThis study investigates the role of eugenol (EUG) on CS-induced acute lung injury (ALI) and how this compound is able to modulate macrophage activity. C57BL/6 mice were exposed to 12 cigarettes/day/5days and treated 15 min/day/5days with EUG. Rat alveolar macrophages (RAMs) were exposed to CSE (5%) and treated with EUG. In vivo, EUG reduced morphological changes inflammatory cells, oxidative stress markers, while, in vitro, it induced balance in the oxidative stress and reduced the pro-inflammatory cytokine release while increasing the anti-inflammatory one. These results suggest that eugenol reduced CS-induced ALI and acted as a modulator of macrophage activity.
- A modified hydrogel production protocol to decrease cellular content(2022) BRAGA, Gabriela Catao Diniz; CAMARGO, Cristina Pires; HARMSEN, Martin Conrad; CORREIA, Aristides Tadeu; SOUZA, Sonia; SEELAENDER, Marilia; NUNES, Viviane Araujo; SANTOS, Jeniffer Farias dos; NERI, Elida Adalgisa; VALADAO, Iuri Cordeiro; MOREIRA, Luiz Felipe Pinho; GEMPERLI, RolfPurpose: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. Methods: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation. Results: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days. Conclusion: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.