DENISE AYA OTSUKI
Projetos de Pesquisa
Unidades Organizacionais
LIM/08 - Laboratório de Anestesiologia, Hospital das Clínicas, Faculdade de Medicina
2 resultados
Resultados de Busca
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- Cyclooxygenases 1 and 2 inhibition and analgesic efficacy of dipyrone at different doses or meloxicam in cats after ovariohysterectomy(2021) PEREIRA, Marco A. A.; CAMPOS, Karina D.; GONCALVES, Lucas A.; SANTOS, Rosana S. T. dos; FLOR, Patricia B.; AMBROSIO, Aline M.; OTSUKI, Denise A.; MATERA, Julia M.; GOMES, Cristina O. M. S.; FANTONI, Denise T.Objective To evaluate the cyclooxygenases (COX) inhibition, adverse effects and analgesic efficacy of dipyrone or meloxicam in cats undergoing elective ovariohysterectomy. Study design Prospective, blinded, randomized, clinical study. Animals A total of 30 healthy young cats. Methods The cats were randomly assigned to three postoperative groups: D25 (dipyrone 25 mg kg(-1) every 24 hours), D12.5 (dipyrone 12.5 mg kg(-1) every 12 hours) and M (meloxicam 0.1 mg kg(-1) every 24 hours). In the first 24 hours, the drugs were administered intravenously (IV), and then orally for 6 (dipyrone) or 3 days (meloxicam). Prostanoids thromboxane B-2 and prostaglandin E-2 concentrations served as indicators of COX activity and, with physiological variables and pain and sedation scores, were measured for 24 hours after first analgesic administration. Rescue analgesia (tramadol, 2 mg kg(-1) IV) was provided if Glasgow feline composite measure pain scale (CMPS-Feline) >= 5. Laboratory tests included symmetric dimethylarginine and adverse effects were evaluated regularly up to 7 and 10 days after surgery, respectively. Parametric and nonparametric data were analyzed with two-way ANOVA and Kruskal-Wallis tests, respectively (p < 0.05). Results In the first half hour after analgesic administration, COX-1 activity was close to zero and remained significantly lower than before drug administration for 24 hours in all groups. The inhibition of COX-2 activity was significant for 30 minutes in all groups and up to 4 hours in group M. No alterations in laboratory tests or significant adverse effects were observed. Pain scores and need for rescue analgesia did not differ statistically among groups. Conclusions Dipyrone at both doses and meloxicam provided a nonselective inhibition of COX-1 and -2 activities and effective analgesia without causing significant adverse effects or laboratory tests alterations.
- Effects of bilateral topical administration of repeated doses of rocuronium bromide as a mydriatic agent in Amazon parrots (Amazona aestiva and Amazona amazonica)(2021) DONGO, Pamela S. J.; PINTO, Debora G.; GUIMARAES, Marta B.; OTSUKI, Denise A.; SAFATLE, Angelica M. V.; BOLZAN, Aline A.The effects of bilateral topical application of repeated doses of rocuronium in parrots are unknown, motivating this research. These effects were evaluated in 24 healthy adult Amazon parrots (12 Amazona aestiva and 12 Amazona amazonica). Three different doses of rocuronium bromide (10 mg/mL) were applied with a micropipette onto the cornea: a single instillation of 20 mu L/eye; two instillations of 20 mu L/eye, spaced 15 minutes apart; and three instillations of 20 mu L/eye, spaced 15 minutes apart. Pupillary diameter (PD) was measured with a digital caliper before treatments (TO), every 15 minutes during the first hour postadministration (T15, T30, T45, T60), hourly for 6 hours (T120, T180, T240, T300, T360), and after 24 hours (T24h). Significant differences were observed between TO and T15 -T120 with a single instillation and between TO and T15-T360 with double and triple instillations. The maximum median PDs were 3.47 mm (T45 one instillation), 3.88 mm (T45 two instillations), and 4.09 mm (T30 three instillations). PD returned to baseline at T180 with one instillation and at T24h with double and triple instillations. All protocols showed similar PD values at TO and T24h. Transient lower eyelid paresis was the only adverse effect observed. Bilateral topical administrations of two and three repeated rocuronium bromide doses produced adequate mydriasis in Amazon parrots and may be indicated for longer ophthalmic procedures.