PROTASIO LEMOS DA LUZ
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cardio-Pneumologia, Faculdade de Medicina - Docente
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Translational Medicine and Implementation Science: How to Transform What We Know Into What We Do(2022) LUZ, Protasio Lemos da; LAURINDO, Francisco Rafael Martins
conferenceObject GUT MICROBIOTA DIFFERS IN PHYSICALLY ACTIVE AND INACTIVE PATIENTS WITH STABLE CORONARY HEART DISEASE(2022) HAAS, Elisa; SANTOS, Andrey; LEMOS JUNIOR, Wilson J.; TREU, Laura; LAURINDO, Francisco R.; LUZ, Protasio Lemos Da- A red wine intervention does not modify plasma trimethylamine N-oxide but is associated with broad shifts in the plasma metabolome and gut microbiota composition(2022) HAAS, Elisa A.; SAAD, Mario J. A.; SANTOS, Andrey; VITULO, Nicola; LEMOS JR., Wilson J. F.; MARTINS, Aline M. A.; PICOSSI, Carolina R. C.; FAVARATO, Desiderio; GASPAR, Renato S.; MAGRO, Daniela O.; LIBBY, Peter; LAURINDO, Francisco R. M.; LUZ, Protasio L. DaBackground Gut microbiota profiles are closely related to cardiovascular diseases through mechanisms that include the reported deleterious effects of metabolites, such as trimethylamine N-oxide (TMAO), which have been studied as diagnostic and therapeutic targets. Moderate red wine (RW) consumption is reportedly cardioprotective, possibly by affecting the gut microbiota. Objectives To investigate the effects of RW consumption on the gut microbiota, plasma TMAO, and the plasma metabolome in men with documented coronary artery disease (CAD) using a multiomics assessment in a crossover trial. Methods We conducted a randomized, crossover, controlled trial involving 42 men (average age, 60 y) with documented CAD comparing 3-wk RW consumption (250 mL/d, 5 d/wk) with an equal period of alcohol abstention, both preceded by a 2-wk washout period. The gut microbiota was analyzed via 16S rRNA high-throughput sequencing. Plasma TMAO was evaluated by LC-MS/MS. The plasma metabolome of 20 randomly selected participants was evaluated by ultra-high-performance LC-MS/MS. The effect of RW consumption was assessed by individual comparisons using paired tests during the abstention and RW periods. Results Plasma TMAO did not differ between RW intervention and alcohol abstention, and TMAO concentrations showed low intraindividual concordance over time, with an intraclass correlation coefficient of 0.049 during the control period. After RW consumption, there was significant remodeling of the gut microbiota, with a difference in beta diversity and predominance of Parasutterella, Ruminococcaceae, several Bacteroides species, and Prevotella. Plasma metabolomic analysis revealed significant changes in metabolites after RW consumption, consistent with improved redox homeostasis. Conclusions Modulation of the gut microbiota may contribute to the putative cardiovascular benefits of moderate RW consumption. The low intraindividual concordance of TMAO presents challenges regarding its role as a cardiovascular risk biomarker at the individual level. This study was registered at clinical trials.gov as NCT03232099.
- The effects of macronutrients composition on hormones and substrates during a meal tolerance test in drug-naive and sitagliptin-treated individuals with type 2 diabetes: a randomized crossover study(2022) SCHREIBER, Cristina da Silva; RAFACHO, Alex; SILVERIO, Renata; BETTI, Roberto; LERARIO, Antonio Carlos; LOTENBERG, Ana Maria Pita; RAHMANN, Klara; OLIVEIRA, Carolina Piras de; WAJCHENBERG, Bernardo Leo; LUZ, Protasio Lemos daObjective', To evaluate the effect of sitagliptin treatment in early type 2 diabetes mellitus (T2DM) and the impact of different macronutrient compositions on hormones and substrates during meal tolerance tests (MTT). Materials and methods: Half of the drug-naive patients with T2DM were randomly assigned for treatment with 100 mg of sitagliptin, q.d., or placebo for 4 weeks and then submitted to 3 consecutive MTT intercalated every 48 h.The MTTs differed in terms of macronutrient composition, with 70% of total energy from carbohydrates, proteins, or lipids. After 4 weeks of washout, a crossover treatment design was repeated. Both patients and researchers were blinded, and a repeated-measures ANOVA was employed for statistical analysis. Results: Sitagliptin treatment reduced but did not normalize fasting and post-meal glucose values in the three MTTs, with lowered area-under-glucose-curve values varying from 7% to 15%. The sitagliptin treatment also improved the insulinogenic index (+86%) and the insulin/glucose (+25%), glucagon-like peptide-1/glucose (+46%) incremental area under the curves. Patients with early T2DM maintained the lowest glucose excursion after a protein- or lipid-rich meal without any major change in insulin, C-peptide, glucagon, or NEFA levels. Conclusion: We conclude that sitagliptin treatment is tolerable and contributes to better control of glucose homeostasis in earlyT2DM, irrespective of macronutrient composition.The blood glucose excursion during meal ingestion is minimal in protein- or fat-rich meals, which can be a positive ally for the management of T2DM.
- Physician-Researcher, Medical Practice and Research: The Importance of the Physician-Researcher in Current Medicine(2022) LUZ, Protasio Lemos da
conferenceObject RED WINE REMODELS GUT MICROBIOTA INDEPENDENTLY OF TRIMETHYLAMINE N-OXIDE LEVELS(2022) HAAS, Elisa; SAAD, Mario J.; SANTOS, Andrey; VITULO, Nicola; LEMOS JR., Wilson; MARTINS, Aline; PICOSSI, Carolina R.; FAVARATO, Desiderio; LIBBY, Peter; LAURINDO, Francisco; LUZ, Protasio Lemos Da