BEATRIZ MANGUEIRA SARAIVA RAMANHOLO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
16 resultados
Resultados de Busca
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- Airway remodeling is reversed by aerobic training in a murine model of chronic asthma(2015) SILVA, R. A.; ALMEIDA, F. M.; OLIVO, C. R.; SARAIVA-ROMANHOLO, B. M.; MARTINS, M. A.; CARVALHO, C. R. F.The aim of this study was to investigate if the aerobic training (AT) reverses airway remodeling (AR) in an asthma model. BALB/c were divided into four groups: control (unsensitized and untrained); ovalbumin (OVA: sensitized and untrained); AT (unsensitized and trained) and OVA+AT. Allergic inflammation was induced with intraperitoneal and OVA inhalation. AT (low intensity; 5x/week; 60min/session) was performed at 7, 15, and 30 days. Leukocyte counting in the bronchoalveolar lavage fluid; the expression of IL-5, eotaxin, RANTES, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1); AR features (airway smooth muscle, epithelium thickness, collagen and elastic fibers, mucus production); and AR inducers (transforming growing factor-beta, osteopontin, vascular endothelial growth factor). OVA induced an increase in leukocyte airway migration and increased AR features (P<0.05). After 7 days, AT reversed the OVA-induced eosinophil and macrophage airway migration, the expression of IL-5, eotaxin, RANTES, ICAM-1, VCAM-1, and all AR inducers. However, total reversion of the AR features and inducers and airway inflammation occurred only after 15 days of AT compared with the OVA groups (P<0.05) and the effects were maintained until the 30th day. AT reverses AR after 15 days and this effect is preceded by the inhibition of leukocyte migration and occurs simultaneously with the reduction in the expression of inflammatory mediators and AR inducers.
- Comparison of the Effects of Aerobic Conditioning Before and After Pulmonary Allergic Inflammation(2015) SILVA, Ronaldo Aparecido da; ALMEIDA, Francine Maria; OLIVO, Clarice Rosa; SARAIVA-ROMANHOLO, Beatriz Mangueira; PERINI, Adenir; MARTINS, Milton Arruda; CARVALHO, Celso Ricardo FernandesThe aim of this study is to compare the effects of aerobic conditioning (AC) before (ACBS) and after (ACAS) allergic sensitization. BALB/c mice were divided into two main groups: ACBS and ACAS. Each groups was divided into subgroups: control (nonsensitized/nontrained), AC (nonsensitized/trained), ovalbumin (OVA) (sensitized/nontrained), AC + OVA (trained/sensitized), and OVA + AC (sensitized/trained). Sensitization was induced using OVA and AC performed in treadmill (moderate intensity). We examined IgE and IgG(1) levels, eosinophil counting, expression of Th1 (interleukin (IL)-2, IFN-alpha) and Th2 cytokines (IL-4, IL-5, IL-13), IL-10, vascular endothelial growth factor (VEGF), and airway remodeling. IgE and IgG(1) were decreased only when exercise was performed before sensitization (ACBS); however, there was a decrease of eosinophils, Th2 cytokines, VEGF, and airway remodeling and increase in IL-10 in either ACBS or ACAS groups. Our results demonstrate that aerobic conditioning reduces Th2 response before and after sensitization by increasing IL-10 while the production of anaphylactic antibodies is reduced only when exercise is performed before sensitization.
conferenceObject IL-10 and IL-1ra mediates OVA-induced Th2 airway allergic response at short and long-term(2013) SILVA, Ronaldo Aparecido; ALMEIDA, Francine Maria; OLIVO, Clarice Rosa; SARAIVA, Beatriz Mangueira; PERINI, Adenir; MARTINS, Milton Arruda; CARVALHO, Celso Ricardo FernandesconferenceObject A unique session of aerobic exercise does not decrease pulmonary inflammation in a murine mice model of asthma(2012) SILVA, Ronaldo Aparecido da; ALMEIDA, Francine Maria; OLIVO, Clarice Rosa; SARAIVA, Beatriz Mangueira; MARTINS, Milton A.; CARVALHO, Celso Ricardo FernandesconferenceObject Aerobic training reverses the inhibition in the expression of glucocorticoid receptors and decreases the Th2 allergic inflammation in asthma model(2013) SILVA, Ronaldo Aparecido; ALMEIDA, Francine Maria; OLIVO, Clarice Rosa; SARAIVA, Beatriz Mangueira; MARTINS, Milton Arruda; CARVALHO, Celso Ricardo Fernandes- Occupational asthma and allergic asthma: differences in the physical activity level, clinical control, and airway inflammation(2023) SILVA, Ronaldo Aparecido Da; FREITAS, Lucas Rodrigues Silva; ALMEIDA, Francine Maria De; GALVAO, Clovis Eduardo Santos; NOGUEIRA, Soraia Felix; BEZERRA, Suellen Karoline Moreira; CARVALHO, Celso Ricardo Fernades De; ARAUJO, Kaique Alves De; CRUZ, Fabiola Matos Da; AGONDI, Rosana Camara; MARTINS, Milton Arruda; TIBERIO, Iolanda De Fatima Lopes Calvo; SARAIVA-ROMANHOLO, Beatriz Mangueira
conferenceObject Aerobic training decrease bronchial hyperresponsiveness and systemic inflammation in patients with moderate or severe asthma: A randomized controlled trial(2014) CARVALHO, Celso R. F.; FRANCA-PINTO, Andrezza; MENDES, Felipe A. R.; STELMACH, Rafael; CUKIER, Alberto; AGONDI, Rosana C.; MARTINS, Milton A.; SARAIVA-ROMANHOLO, Beatriz M.; KALIL, Jorge; GIAVINA-BIANCHI, Pedro- Aerobic training decreases bronchial hyperresponsiveness and systemic inflammation in patients with moderate or severe asthma: a randomised controlled trial(2015) FRANCA-PINTO, Andrezza; MENDES, Felipe A. R.; CARVALHO-PINTO, Regina Maria de; AGONDI, Rosana Camara; CUKIER, Alberto; STELMACH, Rafael; SARAIVA-ROMANHOLO, Beatriz M.; KALIL, Jorge; MARTINS, Milton A.; GIAVINA-BIANCHI, Pedro; CARVALHO, Celso R. F.Background The benefits of aerobic training for the main features of asthma, such as bronchial hyperresponsiveness (BHR) and inflammation, are poorly understood. We investigated the effects of aerobic training on BHR (primary outcome), serum inflammatory cytokines (secondary outcome), clinical control and asthma quality of life (Asthma Quality of Life Questionnaire (AQLQ)) (tertiary outcomes). Methods Fifty-eight patients were randomly assigned to either the control group (CG) or the aerobic training group (TG). Patients in the CG (educational programme+breathing exercises (sham)) and the TG (same as the CG+aerobic training) were followed for 3 months. BHR, serum cytokine, clinical control, AQLQ, induced sputum and fractional exhaled nitric oxide (FeNO) were evaluated before and after the intervention. Results After 12 weeks, 43 patients (21 CG/22 TG) completed the study and were analysed. The TG improved in BHR by 1 doubling dose (dd) (95% CI 0.3 to 1.7 dd), and they experienced reduced interleukin 6 (IL-6) and monocyte chemoattractant protein 1 (MCP-1) and improved AQLQ and asthma exacerbation (p<0.05). No effects were seen for IL-5, IL-8, IL-10, sputum cellularity, FeNO or Asthma Control Questionnaire 7 (ACQ-7; p>0.05). A within-group difference was found in the ACQ-6 for patients with non-well-controlled asthma and in sputum eosinophil and FeNO in patients in the TG who had worse airway inflammation. Conclusions Aerobic training reduced BHR and serum proinflammatory cytokines and improved quality of life and asthma exacerbation in patients with moderate or severe asthma. These results suggest that adding exercise as an adjunct therapy to pharmacological treatment could improve the main features of asthma.
conferenceObject Physical training for asthmatics: Which patients obtain most clinical benefits?(2012) MENDES, Felipe; PINTO, Andrezza; SARAIVA-ROMANHOLO, Beatriz; STELMACH, Rafael; CUKIER, Alberto; GIAVINA-BIANCHI, Pedro; MARTINS, Milton; CARVALHO, CelsoconferenceObject Airways remodeling is reversed by aerobic training in an asthma animal model(2013) SILVA, Ronaldo Aparecido; ALMEIDA, Francine Maria; OLIVO, Clarice Rosa; SARAIVA, Beatriz Mangueira; MARTINS, Milton Arruda; CARVALHO, Celso Ricardo Fernandes