BEATRIZ MANGUEIRA SARAIVA RAMANHOLO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
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- Inflammatory response to Streptococcus pneumoniae was attenuated by moderate aerobic exercise training(2020) CASTRO, Thamyres; LAIA, Roseane M.; MENDONCA, Luana D.; TRINDADE, Pietro Rocha; SOUZA, Jessica C.; SARAIVA-ROMANHOLO, Beatriz M.; OLIVEIRA, Maria Leonor S.; MIYAGI, Eliane N.; PRADO, Carla M.; TIBERIO, Iolanda F. C. L.; OLIVO, Clarice Rosa
- Diphteria-tetanus-pertussis vaccine reduces specific IgE, inflammation and remodelling in an animal model of mite-induced respiratory allergy(2020) AUN, Marcelo Vivolo; ALMEIDA, Francine Maria de; SARAIVA-ROMANHOLO, Beatriz Mangueira; MARTINS, Milton de Arruda; KALIL, Jorge; ARANTES-COSTA, Fernanda Magalhaes; GIAVINA-BIANCHI, PedroBackground: Adjuvants, such as bacterial lipopolysaccharides, have been studied to improve the efficacy of allergen-specific immunotherapy. The Bordetella pertussis (Pw) vaccine has been shown to have a protective role in ovalbumin-induced asthma models. However, its role in allergy to mites is unknown. We evaluated the effects of the diphtheria-tetanus-pertussis (DTPw) vaccine on a murine model of respiratory allergy induced by Dermatophagoides pteronyssinus (Derp). Methods: In a 30-day protocol, BALB/c mice were immunized subcutaneously with saline or Derp, alone or in combination with diphtheria-tetanus (DT) or DTPw vaccines (days 0, 7 and 14). Subsequently, they underwent a daily intranasal challenge with saline or Derp (days 22-28) and were then sacrificed (day 29). We evaluated serum-specific immunoglobulins, bronchoalveolar lavage (BAL) cellularity, remodelling of the lower airways, density of polymorphonuclear leukocytes (PMNs) and acidic nasal mucus content. Results: The animals sensitized with Derp produced high levels of specific immunoglobulins, increased density of PMNs and nasal mucus content, and elevated BAL cellularity and remodelling. Vaccines led to a reduction in IgE levels, with the Derp-DTPw group being similar to the saline groups. The vaccinated groups had reductions of BAL cellularity and remodelling, with more expressive results in the Derp-DTPw group compared to the Derp-DT group. The DT and DTPw vaccines inhibited the nasal PMN infiltrate, and DTPw modulated the production of acidic nasal mucus. Conclusions: The DTPw vaccine reduced serum specific IgE, nasal and pulmonary inflammation and remodelling of the lower airways.
- High Intensity Interval Training control inflammation in mice exposed to Streptococus pneumoniae(2020) CASTRO, Thamyres B. P.; LAIA, Roseane M.; MENDONCA, Luana D.; TRINDADE, Pietro Rocha; SOUZA, Jessica C.; SARAIVA-ROMANHOLO, Beatriz M.; OLIVEIRA, Maria Leonor S.; MIYAGI, Eliane N.; PRADO, Carla M.; TIBERIO, Iolanda F. C. L.; OLIVO, Clarice Rosa
- Randomized trial of physiotherapy and hypertonic saline techniques for sputum induction in asthmatic children and adolescents(2020) FELICIO-JUNIOR, Egberto Luiz; BARNABE, Viviani; ALMEIDA, Francine Maria de; AVONA, Monise Dematte; GENARO, Isabella Santos de; KURDEJAK, Adriana; ELLER, Miriam Cardoso Neves; VERGANID, Karina Pierantozzi; RODRIGUES, Joaquim Carlos; TIBERIO, Iolanda de Fatima Lopes Calvo; MARTINS, Milton de Arruda; SARAIVA-ROMANHOLO, Beatriz MangueiraOBJECTIVES: This study aimed to analyze the efficiency of physiotherapy techniques in sputum induction and in the evaluation of pulmonary inflammation in asthmatic children and adolescents. Although hypertonic saline (HS) is widely used for sputum induction (SI), specific techniques and maneuvers of physiotherapy (P) may facilitate the collection of mucus in some asthmatic children and adolescents. METHODS: A randomized crossover study was performed in patients with well-controlled asthma, and 90 sputum samples were collected. Children and adolescents were assessed using spirometry and randomized at entry into one of three sputum induction techniques: (i) 3% hypertonic saline - HS technique; (ii) physiotherapy (oscillatory positive expiratory pressure, forced expiration, and acceleration of expiratory flow) - P technique; and (iii) hypertonic saline + physiotherapy - HSP technique. ClinicalTrials.gov: NCT03136042. RESULTS: The total cells (mL) and the percentage (%) of differential inflammatory cells were similar in all techniques. The sputum weight (g) in the HSP technique was significantly higher than that in the HS technique. In all techniques, the percentage of viable cells was >50%, and there was no difference between the HS and P techniques. Moreover, sputum induction did not cause any alterations in the pulmonary function of patients. CONCLUSION: The physiotherapy sputum collection technique was effective in obtaining viable cells from mucus samples and yielded the same amount of sputum as the gold standard technique (hypertonic saline). In addition, the physiotherapy maneuvers were both safe and useful for sputum induction in asthmatic children and adolescents with well-controlled asthma.
- High intensity interval training control inflammation in a lung allergic model(2020) TRINDADE, Pietro da Rocha; CASTELO, Thais F. F.; CASTRO, Thamyres B. P.; LAIA, Roseane M.; MENDONCA, Luana D.; SOUZA, Jessica C.; SARAIVA-ROMANHOLO, Beatriz M.; PRADO, Carla M.; TIBERIO, Iolanda F. C. L.; OLIVO, Clarice Rosa
- Effects of Aerobic Training Versus Breathing Exercises on Asthma Control: A Randomized Trial(2020) EVARISTO, Karen B.; MENDES, Felipe Augusto Rodrigues; SACCOMANI, Milene G.; CUKIER, Alberto; CARVALHO-PINTO, Regina M.; RODRIGUES, Marcos R.; SANTAELLA, Danilo F.; SARAIVA-ROMANHOLO, Beatriz M.; MARTINS, Milton A.; CARVALHO, Celso R. F.BACKGROUND: Aerobic training and breathing exercises are interventions that improve asthma control. However, the outcomes of these 2 interventions have not been compared. OBJECTIVE: To compare the effects of aerobic training versus breathing exercises on clinical control (primary outcome), quality of life, exercise capacity, and airway inflammation in outpatients with moderate-to-severe asthma. METHODS: Fifty-four asthmatics were randomized into either the aerobic training group (AG, n = 29) or the breathing exercise group (BG, n = 25). Both interventions lasted for 24 sessions (2/week, 40 minutes/session). Asthma clinical control (Asthma Control Questionnaire = ACQ]), quality of life (Asthma Quality of Life Questionnaire), asthma symptom-free days (ASFD), airway inflammation, exercise capacity, psychological distress (Hospital Anxiety and Depression Scale), daily-life physical activity (DLPA), and pulmonary function were evaluated before, immediately after, and 3 months after the intervention. RESULTS: Both interventions presented similar results regarding the ACQ score, psychological distress, ASFD, DLPA, and airway inflammation (P >.05). However, participants in the AG were 2.6 times more likely to experience clinical improvement at the 3-month follow-up than participants in the BG (P =.02). A greater proportion of participants in the AG also presented a reduction in the number of days without rescue medication use compared with BG (34% vs 8%; P = .04). CONCLUSIONS: Outpatients with moderate-to-severe asthma who participated in aerobic training or breathing exercise programs presented similar results in asthma control, quality of life, asthma symptoms, psychological distress, physical activity, and airway inflammation. However, a greater proportion of participants in the AG presented improvement in asthma control and reduced use of rescue medication. (C) 2020 American Academy of Allergy, Asthma & Immunology
- Streptococcus pneumoniae augments inflammatory process in asthma model without changing Th2 profile(2020) LAIA, Roseane M.; CASTRO, Thamyres B. P.; SANTOS, Camila A.; SARAIVA-ROMANHOLO, Beatriz M.; OLIVEIRA, Maria Leonor S.; MIYAGI, Eliane N.; TIBERIO, Iolanda F. C. L.; OLIVO, Clarice Rosa
- Effect of anti-IL17 and/or Rho-kinase inhibitor treatments on vascular remodeling induced by chronic allergic pulmonary inflammation(2020) SANTOS, Tabata M. dos; RIGHETTI, Renato F.; REZENDE, Bianca G.; CAMPOS, Elaine C.; CAMARGO, Leandro do N.; SARAIVA-ROMANHOLO, Beatriz M.; FUKUZAKI, Silvia; PRADO, Carla M.; LEICK, Edna A.; MARTINS, Milton A.; TIBERIO, Iolanda F. L. C.Background and aims: Expansion and morphological dysregulation of the bronchial vascular network occurs in asthmatic airways. Interleukin (IL) -17 and Rho-kinase (ROCK) are known to act in inflammation control and remodeling. Modulation of Rho-kinase proteins and IL-17 may be a promising approach for the treatment of asthma through the control of angiogenesis. Our objective was to analyze the effects of treatment with anti-IL17 and/or Rho-kinase inhibitor on vascular changes in mice with chronic allergic pulmonary inflammation. Methods: Sixty-four BALB/c mice, with pulmonary inflammation induced by ovalbumin were treated with anti-IL17A (7.5/mu g per dose, intraperitoneal) and/or Rho-kinase inhibitor (Y-27632-10 mg/kg, intranasal), 1 h before each ovalbumin challenge (22, 24, 26, and 28/days). Control animals were made to inhale saline. At the end of the protocol, lungs were removed, and morphometric analysis was performed to quantify vascular inflammatory, remodeling, and oxidative stress responses. Results: Anti-IL17 or Rho-kinase inhibitor reduced the number of CD4(+), CD8(+), dendritic cells, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, Rho-kinase 1 and 2, transforming growth factor (TGF-beta), vascular endothelial growth factor (VEGF), nuclear factor (NF)-KappaB, iNOS, metalloproteinase (MMP)-9, MMP-12, metalloproteinase inhibitor-1 (TIMP-1), FOXP-3, signal transducer and activator of transcription 1 (STAT1) and phospho-STAT1-positive cells, and actin, endothelin-1, isoprostane, biglycan, decorin, fibronectin and the collagen fibers volume fraction compared with the ovalbumin group (p < 0.05). The combination treatment, when compared with anti-IL17, resulted in potentiation of decrease in the number of IL1 beta- and dendritic cells-positive cells. When we compared the OVA-RHO inhibitor-anti-IL17 with OVA-RHO inhibitor we found a reduction in the number of CD8(+) and IL-17, TGF-beta, and phospho-STAT1-positive cells and endothelin-1 in the vessels (p < 0.05). There was an attenuation in the number of ROCK 2-positive cells in the group with the combined treatment when compared with anti-IL17 or Rho-kinase inhibitor-treated groups (p < 0.05). Conclusion: We observed no difference in angiogenesis after treatment with Rho-kinase inhibitor and anti-IL17. Although the treatments did not show differences in angiogenesis, they showed differences in the markers involved in the angiogenesis process contributing to inflammation control and vascular remodeling. The reviews of this paper are available via the supplemental material section.