PAULO SAMPAIO GUTIERREZ
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
3 resultados
Resultados de Busca
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- Associations between clinical features and therapy with macrophage subpopulations and T cells in inflammatory lesions in the aorta from patients with Takayasu arteritis(2020) SANTOS, J. P. dos; NETO, R. Artigiani; MANGUEIRA, C. L. P.; FILIPPI, R. Z.; GUTIERREZ, P. S.; WESTRA, J.; BROUWER, E.; SOUZA, A. W. S. deTakayasu arteritis (TAK) is a large-vessel granulomatous vasculitis; the inflammatory infiltration in arteries comprises macrophages, multi-nucleated giant cells, CD4(+)and CD8(+)T cells, gamma delta T cells, natural killer (NK) cells and neutrophils. However, it is unknown which subtype of macrophages predominates. This study aims to evaluate macrophages subpopulations in the aorta in TAK. Immunohistochemistry was performed in the aorta from TAK patients (n = 22), patients with atherosclerotic disease (n = 9) and heart transplant donors (n = 8) using the markers CD68, CD86, CD206, CD3, CD20 and CD56. Active disease was observed in 54 center dot 5% of patients and active histological lesions were found in 40 center dot 9%. TAK patients presented atherosclerotic lesions in 27 center dot 3% of cases. The frequency of macrophages, M1 macrophages, T, B and NK cells was higher in the aorta from TAK and atherosclerotic patients compared to heart transplant donors. In TAK, macrophages and T cells were the most abundant cells in the aorta, and the expression of CD206 was higher than CD86 (P = 0 center dot 0007). No associations were found between the expression of cell markers and active disease or with atherosclerotic lesions. In TAK patients, histological disease activity led to higher T cell counts than chronic fibrotic lesions (P = 0.030), whereas prednisone use was associated with lower T cell counts (P = 0 center dot 035). In conclusion, M1 macrophages were more frequent in TAK and atherosclerotic patients compared to heart transplant donors, while M2 macrophages dominated M1 macrophages in TAK. T cells were associated with histological disease activity and with prednisone use in TAK.
- Emerging technologies: polymer-free phospholipid encapsulated sirolimus nanocarriers for the controlled release of drug from a stent-plus-balloon or a stand-alone balloon catheter(2013) LEMOS, Pedro A.; FAROOQ, Vasim; TAKIMURA, Celso K.; GUTIERREZ, Paulo S.; VINNANI, Renu; KOLODGIE, Frank; CHRISTIANS, Uwe; KHARLAMOV, Alexander; DOSHI, Manish; SOJITRA, Prakash; BEUSEKOM, Heleen M. M. van; SERRUYS, Patrick W.Drug-eluting stents have proven to be effective in reducing the risk of late restenosis. In order to achieve a controlled and prolonged release of the antiproliferative agent, current drug-eluting stents utilise various biodegradable as well as non-erodible polymeric blends to coat the stent surface and to serve as drug carriers. The utilisation of polymeric compounds in current drug-eluting stents may eventually limit their performance as well as their clinical applicability due to the potential induction of undesirable local reactions. The development of alternative, polymer-free drug carriers has the potential to overcome some of the limitations of current drug-eluting stent formulations. Moreover, improvements in drug carriers may also result in an expansion of the technological possibilities for other intravascular drug delivery systems, such as metal-free or even implant-free solutions. This article describes the structure and the preclinical validation profile of a novel phospholipid encapsulated sirolimus nanocarrier, used as a coating in two formulations: a coronary stent-plus-balloon system and a stand-alone balloon catheter. The nanoparticles provided a stable, even and homogenous coating to the devices in both formulations. Dose-finding studies allowed the most appropriate identification of the best nanoparticle structure associated with an extremely efficient transfer of drug to all layers of the vessel wall, achieving high tissue concentrations that persisted days after the application, with low systemic drug leaks.
conferenceObject MACROPHAGE SUBPOPULATIONS IN INFLAMMATORY LESIONS IN ARTERIES FROM TAKAYASU ARTERITIS PATIENTS(2019) SANTOS, Joao dos; NETO, Ricardo Artigiani; MANGUEIRA, Cristovao; FILIPPI, Renee; GUTIERREZ, Paulo; WESTRA, Johanna; BROUWER, Elisabeth; SOUZA, Alexandre Wagner de