EDECIO CUNHA NETO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina - Líder
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/60 - Laboratório de Imunologia Clínica e Alergia, Hospital das Clínicas, Faculdade de Medicina - Líder
24 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 24
conferenceObject Benznidazole treatment is associated with Trypanosoma cruzi blood PCR negativity and less cardiac lesions in Chagas disease: NIH SaMitrop Study(2017) CARDOSO, C. S.; SABINO, E. C.; OLIVEIRA, C. D. L.; OLIVEIRA, L. C.; FERREIRA, A. M.; BIERRENBACH, A. L.; SILVA, J. L. P.; COLOSIMO, E. A.; CUNHA-NETO, E.; RIBEIRO, A. L. P.conferenceObject Whole exome sequencing of Chagas disease cardiomyopathy families reveals accumulation of rare variants in mitochondrial and inflammation-associated genes(2019) CUNHA-NETO, E.; MARQUET, S.; FRADE, A. Farage; FERREIRA, A. Mota; OUARHACHE, M.; IANNI, B.; FERREIRA, L. Rodrigues Pinto; RIGAUD, V. Oliveira-Carvalho; ALMEIDA, R. Ribeiro; CANDIDO, D.; TORRES, M.; GALLARDO, F.; FERNANDES, R.; MADY, C.; BUCK, P.; CARDOSO, C.; SANTOS-JUNIOR, O. R.; OLIVEIRA, L. C.; OLIVEIRA, C. D. L.; NUNES, M. do Carmo; ABEL, L.; KALIL, J.; RIBEIRO, A. L. P.; SABINO, E. C.; CHEVILLARD, C.conferenceObject HIV CD4 Based Vaccine Increases CD8 T Cell Responses and Confers Protection Against Challenge with Recombinant Vaccinia Encoding HIV Gag-Pol Proteins(2013) RIBEIRO, S. P.; ROSA, D. S.; KALIL, J.; CUNHA-NETO, E.- CD4+T cells from HIV-1-infected patients recognize wild-type and mutant human immunodeficiency virus-1 protease epitopes(2011) MULLER, N. G.; ALENCAR, R.; JAMAL, L.; HAMMER, J.; SIDNEY, J.; SETTE, A.; BRINDEIRO, R. M.; KALIL, J.; CUNHA-NETO, E.; MORAES, S. L.P>Human immunodeficiency virus (HIV)-1 protease is a known target of CD8+ T cell responses, but it is the only HIV-1 protein in which no fully characterized HIV-1 protease CD4 epitopes have been identified to date. We investigated the recognition of HIV-1 protease by CD4+ T cells from 75 HIV-1-infected, protease inhibitor (PI)-treated patients, using the 5,6-carboxyfluorescein diacetate succinimidyl ester-based proliferation assay. In order to identify putative promiscuous CD4+ T cell epitopes, we used the TEPITOPE algorithm to scan the sequence of the HXB2 HIV-1 protease. Protease regions 4-23, 45-64 and 73-95 were identified; 32 sequence variants of the mentioned regions, encoding frequent PI-induced mutations and polymorphisms, were also tested. On average, each peptide bound to five of 15 tested common human leucocyte antigen D-related (HLA-DR) molecules. More than 80% of the patients displayed CD4+ as well as CD8+ T cell recognition of at least one of the protease peptides. All 35 peptides were recognized. The response was not associated with particular HLA-DR or -DQ alleles. Our results thus indicate that protease is a frequent target of CD4+ along with CD8+ proliferative T cell responses by the majority of HIV-1-infected patients under PI therapy. The frequent finding of matching CD4+ and CD8+ T cell responses to the same peptides may indicate that CD4+ T cells provide cognate T cell help for the maintenance of long-living protease-specific functional CD8+ T cells.
conferenceObject Gene expression profile of peripheral blood mononuclear cells of recent-onset type 1 diabetes(2018) SANTOS, A. S.; CHEVILLARD, C.; GONFINETTI, N. V.; KALIL, J.; CUNHA-NETO, E.; SILVA, M. R.conferenceObject Repurposed drugs acting on host mechanisms of T. cruzi invasion synergize with Benznidazole: New therapies for Chagas disease(2019) PANDEY, R. P.; NASCIMENTO, M. Savoia; BARRIOS, L.; GIBALDI, D.; LANNES-VIEIRA, J.; KALIL, J.; CUNHA-NETO, E.- Protocol for design, construction, and selection of genome phage (gPhage) display libraries(2021) CARNERO, L. A. Rodriguez; TEIXEIRA, A. A. Reis; TANG, F. H. Fen; KURAMOTO, A.; ALVES, M. J. Manso; COLLI, W.; SETUBAL, J. C.; CUNHA-NETO, E.; PASQUALINI, R.; ARAP, W.; GIORDANO, R. J.This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns. For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021). © 2021 The Author(s)
conferenceObject Myocardial perfusion disturbance precedes LV systolic dysfunction in experimental model of chronic Chagas cardiomyopathy(2018) OLIVEIRA, L. F. L.; THACKERAY, J. T.; TANAKA, D. M.; MARIN-NETO, J. A.; ROMANO, M. M. D.; LOPES, C. D.; BARROS-FILHO, A. C. L.; RIBEIRO, F. F. F.; MEJIA, J.; MALAMUT, C.; BENGEL, F. M.; CUNHA-NETO, E.; HIGUCHI, M. L.; NEKOLLA, S. G.; SIMOES, M. V.conferenceObject Endothelial inflammatory activation is related to myocardial perfusion disturbance in experimental chronic Chagas disease(2022) TANAKA, D. M.; FABRICIO, C. G.; MARIN-NETO, J. A.; BARROS-FILHO, A. C. L.; LOPES, C. D.; OLIVEIRA, L. F. L.; MEJIA, J.; ALMEIDA, R. R.; BATAH, S. S.; NEKOLLA, S. G.; HIGUCHI, M. L.; CUNHA-NETO, E.; FABRO, A. T.; ROMANO, M. M.; SIMOES, M. V.conferenceObject Rest myocardial perfusion disturbance is related to inflammation but not to fibrosis inexperimental chronic chagas cardiomyopathy(2013) OLIVEIRA, L. F. L.; CARVALHO, E. E. V.; MEJIA, J.; SANTANA-SILVA, J.; ROMANNO, M. M. D.; CUNHA-NETO, E.; HIGUCHI, M. L.; MACIEL, B. C.; MARIN-NETO, J. A.; SIMOES, M. V.
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