FRANCISCO GARCIA SORIANO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina - Docente
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina - Líder
7 resultados
Resultados de Busca
Agora exibindo 1 - 7 de 7
conferenceObject Anti-inflammatory Effects of Dipeptidyl Peptidase IV (DPPIV) Inhibition in Heart Failure(2015) SALLES, Thiago; ZOGBI, Camila; LIMA, Thais; SORIANO, Francisco; GIRARDI, AdrianaconferenceObject Baroreflex Sensitivity and Mortality in Septic dysfunction(2018) SANTOS, Fernando dos; NOGUEIRA, Antonio Carlos; BISELLI, Paolo; HOSHINO, Wagner; MOSTARDA, Cristiano Teixeira; ANGELIS, Katia De; SORIANO, Francisco Garcia; IRIGOYEN, Maria ClaudiaconferenceObject Increase of splenic lymphocyte apoptosis in septic encephalopathy(2014) JEREMIAS, Isabela; VICTORINO, Vanessa; LIMA, Thais; SORIANO, FranciscoconferenceObject Effect of the tolerance to LPS in the cytokine production and activity of epigenetic enzymes in endotoxemic mice(2013) RIOS, Ester Correia Sarmento; LIMA, Thais Martins De; SORIANO, Francisco GarciaEpigenetic alteration could be associated to pre-conditioning procedures. Chromatin remodeling in LPS tolerance is achieved by histone acetylation. LPS low doses induce a gene hypo-transcription in acute immune response. We investigated how the tolerance to LPS could regulate the epigenetic response in the endotoxemia. Male "Balb C" mice were divided in: C group – control, not manipulated; TD group – induced tolerance and challenged -1mg/Kg LPS during 5 days before the challenge (5mg/Kg LPS); D group – challenged without tolerance - 100μL of NaCl 0.9% during 5 days before the challenge (5mg/kg LPS). Spleens and plasma were collected 48 hours after the challenge. Activity of Histone Acetyltransferases (HAT) and Histone Deacetylases (HDAC) were assessed by colorimetric kits (abcam and biovision); Acetylation of histones H3 and H4 were analyzed by western blotting; cytokines production was measured by ELISA. Results represent the media of 5 animals. Statistical analysis was performed by ANOVA. Production of IL-6, IL-10 and TNF-α was inhibited by the induction of the tolerance compared to D group. HAT and HDAC activities were increased in the TD group compared to C/D groups (p<0.05). H3 acetylated was reduced in TD compared to D. H4 acetylated was maintained at basal levels in TD and D groups. In conclusion, the reduction of cytokines during the tolerance to LPS is associated to H3 acetylation.conferenceObject Investigation of immunological changes in mice subjected to animal model of sepsis(2013) JEREMIAS, Isabela Casagrande; VICTORINO, Vanessa Jacob; BARBEIRO, Hermes Vieira; LIMA, Thais Martins; SORIANO, Francisco GarciaconferenceObject Hypertonic saline acts on septic lung remodeling through nitric oxide-induced FAK activation pathway(2013) PETRONI, Ricardo; BISELLI, Paolo; BARBEIRO, Hermes; KUBO, Suely; SORIANO, FranciscoconferenceObject ACTION OF HYPERTONIC SALINE SOLUTION (NaCl 7,5%) IN PULMONARY FIBROSIS IN A RODENT MODEL OF ENDOTOXEMIA(2012) PETRONI, Ricardo Costa; BISELLI, Paolo; MARTINS, Milton; CSABA, Szabo; SORIANO, FranciscoHypertonic saline solution (HSS, NaCl 7,5%) has shown to modulates immune function favorably and decrease pulmonary injury triggered by endotoxic shock. Therefore, our objective was to investigate the effects of HSS on the mechanism involved in pulmonary fibrosis, in an experimental model of endotoxemic shock. Wistar rats (220–260 g) received lipopolysaccharide - LPS (10mg/kg, intraperitoneal,ip) and volume after 15 minutes. The animals were assigned in four groups (n=7 per group): control group (not subjected to LPS); LPS group (injected with LPS 10mg/kg i.p); HSS group (injected with LPS and treated with hypertonic saline, 4 mL/Kg) and NS group (injected with LPS and treated with normal saline, 34 mL/kg). At 24h after treatment, pulmonary mechanics, type I collagen expression, metalloproteinase 9 expression and activity, and focal adhesion kinase (FAK) were measured. Normal saline increased pulmonary resistance and elastance, compared to other groups (p<0.05). HSS inhibited collagen expression compared to LPS and NS groups and prevented pulmonary injury by decreased MMP9 activity in tissue (p<0.05). Expression of FAK was decreased in HSS groups compared to LPS and NS groups (P<0.05). Therefore, we concluded that treatment of endotoxemic shock with HSS solution can decreased pulmonary fibrosis and injury through FAK pathway.