ROSSANA VERONICA MENDOZA LOPEZ
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- Tolerability of modified gemcitabine/docetaxel (split-dose) in patients with advanced soft tissue sarcomas(2016) AZEVEDO, R. G. M. V. D.; FRAILE, N.; SAADI NETO, E.; LOPEZ, R. V. M.; TOLOI, D.; HOFF, P. M.; FEHER, O.; CAMARGO, V. P. D.; MUNHOZ, R.
- HNdb: an integrated database of gene and protein information on head and neck squamous cell carcinoma(2016) HENRIQUE, Tiago; SILVEIRA, Nelson Jose Freitas da; VOLPATO, Arthur Henrique Cunha; MIOTO, Mayra Mataruco; STEFANINI, Ana Carolina Buzzo; FARES, Adil Bachir; ANDRADE, Joao Gustavo da Silva Castro; MASSON, Carolina; LOPEZ, Rossana Veronica Mendoza; NUNES, Fabio Daumas; KOWALSKI, Luis Paulo; SEVERINO, Patricia; TAJARA, Eloiza HelenaThe total amount of scientific literature has grown rapidly in recent years. Specifically, there are several million citations in the field of cancer. This makes it difficult, if not impossible, to manually retrieve relevant information on the mechanisms that govern tumor behavior or the neoplastic process. Furthermore, cancer is a complex disease or, more accurately, a set of diseases. The heterogeneity that permeates many tumors is particularly evident in head and neck (HN) cancer, one of the most common types of cancer worldwide. In this study, we present HNdb, a free database that aims to provide a unified and comprehensive resource of information on genes and proteins involved in HN squamous cell carcinoma, covering data on genomics, transcriptomics, proteomics, literature citations and also cross-references of external databases. Different literature searches of MEDLINE abstracts were performed using specific Medical Subject Headings (MeSH terms) for oral, oropharyngeal, hypopharyngeal and laryngeal squamous cell carcinomas. A curated gene-to-publication assignment yielded a total of 1370 genes related to HN cancer. The diversity of results allowed identifying novel and mostly unexplored gene associations, revealing, for example, that processes linked to response to steroid hormone stimulus are significantly enriched in genes related to HN carcinomas. Thus, our database expands the possibilities for gene networks investigation, providing potential hypothesis to be tested. Database URL: http://www.gencapo.famerp.br/hndb
- Lymph node or perineural invasion is associated with low miR-15a, miR-34c and miR-199b levels in head and neck squamous cell carcinoma(2016) SOUSA, Lucas O.; SOBRAL, Lays M.; MATSUMOTO, Camila S.; SAGGIORO, Fabiano P.; LOPEZ, Rossana V. M.; PANEPUCCI, Rodrigo A.; CURTI, Carlos; SILVA JR., Wilson A.; GREENE, Lewis J.; LEOPOLDINO, Andreia M.Background: MicroRNAs (miRNAs or miRs) are post-transcriptional regulators of eukaryotic cells and knowledge of differences in miR levels may provide new approaches to diagnosis and therapy. Methods: The present study measured the levels of nine miRs in head and neck squamous cell carcinomas (HNSCC) and determined whether clinical pathological features are associated with differences in miR levels. SET (I2PP2A) and PTEN protein levels were also measured, since their levels can be regulated by miR-199b and miR-21, respectively. Nine miRs (miR-15a, miR-21, miR-29b, miR-34c, miR-100, miR-125b, miR-137, miR133b and miR-199b) were measured by real time qRT-PCR in HNSCC samples from 32 patients and eight resection margins. SET (I2PP2A) and PTEN protein levels were estimated by immunohistochemistry in paired HNSCC tissues and their matched resection margins. Results: In HNSCC, the presence of lymph node invasion was associated with low miR-15a, miR-34c and miR-199b levels, whereas the presence of perineural invasion was associated with low miR-199b levels. In addition, miR-21 levels were high whereas miR-100 and miR-125b levels were low in HNSCC compared to the resection margins. When HNSCC line HN12, with or without knockdown of SET, were transfected with miR-34c inhibitor or miR-34cmimic, the miR-34c inhibitor increased cell invasion capacity while miR-34cmimic decreased the cell invasion. Conclusions: We showed that the levels of specific miRs in tumor tissue can provide insight into the maintenance and progression of HNSCC. General significance: MiRNAs are up-or down-regulated during cancer development and progression; they can be prognosis markers and therapeutic targets in HNSCC. (C) 2016 The Authors.
- Detection of human papillomavirus in laryngeal squamous cell carcinoma: Systematic review and meta-analysis(2016) GAMA, Ricardo Ribeiro; CARVALHO, Andre Lopes; LONGATTO FILHO, Adhemar; SCORSATO, Anderson Paulo; LOPEZ, Rossana V. Mendoza; RAUTAVA, Jaana; SYRJAENEN, Stina; SYRJAENEN, KariBackgroundRecent studies have reported a human papillomavirus (HPV) prevalence of 20% to 30% in laryngeal squamous cell carcinoma (LSCC), although clinical data on HPV involvement remain largely inconsistent, ascribed by some to differences in HPV detection methods or in geographic origin of the studies. ObjectiveTo perform a systematic review and formal meta-analysis of the literature reporting on HPV detection in LSCC. MethodsLiterature was searched from January 1964 until March 2015. The effect size was calculated as event rates (95% confidence interval [CI]), with homogeneity testing using Cochran's Q and I-2 statistics. Meta-regression was used to test the impact of study-level covariates (HPV detection method, geographic origin) on effect size. Potential publication bias was estimated using funnel plot symmetry. ResultsOne hundred seventy nine studies were eligible, comprising a sample size of 7,347 LSCCs from different geographic regions. Altogether, 1,830 (25%) cases tested HPV-positive considering all methods, with effect size of 0.269 (95% CI: 0.242 to 0.297; random-effects model). In meta-analysis stratified by the 1) HPV detection technique and 2) geographic study origin, the between-study heterogeneity was significant only for geographic origin (P = .0001). In meta-regression, the HPV detection method (P = .876) or geographic origin (P = .234) were not significant study-level covariates. Some evidence for publication bias was found only for studies from North America and those using non-polymerase chain reaction methods, with a marginal effect on adjusted point estimates for both. ConclusionsVariability in HPV detection rates in LSCC is explained by geographic origin of study but not by HPV detection method. However, they were not significant study-level covariates in formal meta-regression. Level of EvidenceNA Laryngoscope, 126:885-893, 2016
conferenceObject Human papillomavirus 16 is an independent predictor of better survival among patients with early cervical cancer(2016) GENTA, M. L. N. D.; LEVI, J. E.; MARTINS, T. R.; SADALLA, J. C.; LOPEZ, R. V. M.; CARVALHO, J. P. M.; CARVALHO, J. P.