JOSE EDUARDO KRIEGER
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Cardio-Pneumologia, Faculdade de Medicina - Docente
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/13 - Laboratório de Genética e Cardiologia Molecular, Hospital das Clínicas, Faculdade de Medicina - Líder
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
LIM/13 - Laboratório de Genética e Cardiologia Molecular, Hospital das Clínicas, Faculdade de Medicina - Líder
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- Aerobic Exercise Training-Induced Left Ventricular Hypertrophy Involves Regulatory MicroRNAs, Decreased Angiotensin-Converting Enzyme-Angiotensin II, and Synergistic Regulation of Angiotensin-Converting Enzyme 2-Angiotensin (1-7)(2011) FERNANDES, Tiago; HASHIMOTO, Nara Y.; MAGALHAES, Flavio C.; FERNANDES, Fernanda B.; CASARINI, Dulce E.; CARMONA, Adriana K.; KRIEGER, Jose E.; PHILLIPS, M. Ian; OLIVEIRA, Edilamar M.Aerobic exercise training leads to a physiological, nonpathological left ventricular hypertrophy; however, the underlying biochemical and molecular mechanisms of physiological left ventricular hypertrophy are unknown. The role of microRNAs regulating the classic and the novel cardiac renin-angiotensin (Ang) system was studied in trained rats assigned to 3 groups: (1) sedentary; (2) swimming trained with protocol 1 (T1, moderate-volume training); and (3) protocol 2 (T2, high-volume training). Cardiac Ang I levels, Ang-converting enzyme (ACE) activity, and protein expression, as well as Ang II levels, were lower in T1 and T2; however, Ang II type 1 receptor mRNA levels (69% in T1 and 99% in T2) and protein expression (240% in T1 and 300% in T2) increased after training. Ang II type 2 receptor mRNA levels (220%) and protein expression (332%) were shown to be increased in T2. In addition, T1 and T2 were shown to increase ACE2 activity and protein expression and Ang (1-7) levels in the heart. Exercise increased microRNA-27a and 27b, targeting ACE and decreasing microRNA-143 targeting ACE2 in the heart. Left ventricular hypertrophy induced by aerobic training involves microRNA regulation and an increase in cardiac Ang II type 1 receptor without the participation of Ang II. Parallel to this, an increase in ACE2, Ang (1-7), and Ang II type 2 receptor in the heart by exercise suggests that this nonclassic cardiac renin-angiotensin system counteracts the classic cardiac renin-angiotensin system. These findings are consistent with a model in which exercise may induce left ventricular hypertrophy, at least in part, altering the expression of specific microRNAs targeting renin-angiotensin system genes. Together these effects might provide the additional aerobic capacity required by the exercised heart. (Hypertension. 2011;58:182-189.).
conferenceObject Prevalence, Predictors and Comparison of Spironolactone versus Clonidine as a Fourth Drug for Resistant Hypertension: the Resistant Hypertension Optimal Treatment (ReHOT) study(2016) KRIEGER, Eduardo M.; DRAGER, Luciano F.; GIORGI, Dante M.; PEREIRA, Alexandre C.; BARRETO-FILHO, Jose A.; NOGUEIRA, Armando R.; MILL, Jose G.; KRIEGER, Jose E.- Blood Pressure Polygenic Risk Score Stratifies Individuals With High Risk Of Hypertension In Different Ancestries(2022) TEIXEIRA, Samantha K.; SOUZA, Vinicius; HORTA, Bernardo; PEREIRA, Alexandre; KRIEGER, Jose E.
- Spironolactone Versus Clonidine as a Fourth-Drug Therapy for Resistant Hypertension: The ReHOT Randomized Study (Resistant Hypertension Optimal Treatment)(2018) KRIEGER, Eduardo M.; DRAGER, Luciano F.; GIORGI, Dante M. A.; PEREIRA, Alexandre C.; BARRETO-FILHO, Jose Augusto Soares; NOGUEIRA, Armando R.; MILL, Jose Geraldo; LOTUFO, Paulo A.; AMODEO, Celso; BATISTA, Marcelo C.; BODANESE, Luiz C.; CARVALHO, Antonio C. C.; CASTRO, Iran; CHAVES, Hilton; COSTA, Eduardo A. S.; FEITOSA, Gilson S.; FRANCO, Roberto J. S.; FUCHS, Flavio D.; GUIMARAES, Armenio C.; JARDIM, Paulo C.; MACHADO, Carlos A.; MAGALHAES, Maria E.; MION JR., Decio; NASCIMENTO, Raimundo M.; NOBRE, Fernando; NOBREGA, Antonio C.; RIBEIRO, Antonio L. P.; RODRIGUES-SOBRINHO, Carlos R.; SANJULIANI, Antonio F.; TEIXEIRA, Maria do Carmo B.; KRIEGER, Jose E.The aim of this study is to compare spironolactone versus clonidine as the fourth drug in patients with resistant hypertension in a multicenter, randomized trial. Medical therapy adherence was checked by pill counting. Patients with resistant hypertension (no office and ambulatory blood pressure [BP] monitoring control, despite treatment with 3 drugs, including a diuretic, for 12 weeks) were randomized to an additional 12-week treatment with spironolactone (12.5-50 mg QD) or clonidine (0.1-0.3 mg BID). The primary end point was BP control during office (<140/90 mmHg) and 24-h ambulatory (<130/80 mmHg) BP monitoring. Secondary end points included BP control from each method and absolute BP reduction. From 1597 patients recruited, 11.7% (187 patients) fulfilled the resistant hypertension criteria. Compared with the spironolactone group (n=95), the clonidine group (n=92) presented similar rates of achieving the primary end point (20.5% versus 20.8%, respectively; relative risk, 1.01 [0.55-1.88]; P=1.00). Secondary end point analysis showed similar office BP (33.3% versus 29.3%) and ambulatory BP monitoring (44% versus 46.2%) control for spironolactone and clonidine, respectively. However, spironolactone promoted greater decrease in 24-h systolic and diastolic BP and diastolic daytime ambulatory BP than clonidine. Per-protocol analysis (limited to patients with 80% adherence to spironolactone/clonidine treatment) showed similar results regarding the primary end point. In conclusion, clonidine was not superior to spironolactone in true resistant hypertensive patients, but the overall BP control was low (approximate to 21%). Considering easier posology and greater decrease in secondary end points, spironolactone is preferable for the fourth-drug therapy. Clinical Trial Registration URL: http://www.clinicaltrials.gov. Unique identifier: NCT01643434.
- Germline Mutagenesis Induces Blood Pressure And Heart Rate Variation In Mice Uncovering Novel Insights On The Genesis Of Cardiovascular Disease Risks(2022) TEIXEIRA, Samantha Kuwada; PONTES, Roberto; ZULETA, Luiz Fernando; ROSA, Rogerio; BERTOLINE, Leticia; WANG, Jianhui; XU, Darui; RUSSELL, Jamie L.; ZHAN, Xiaoming; CHOI, Mihwa; TANG, Miao; LI, Xiaohong; LUDWIG, Sara; BEUTLER, Bruce; KRIEGER, Jose E.