EDUARDO MILTON RAMOS SANCHEZ

(Fonte: Lattes)
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LIM/38 - Laboratório de Epidemiologia e Imunobiologia, Hospital das Clínicas, Faculdade de Medicina

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Colaboração internacional: Peru ×

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  • article 10 Citação(ões) na Scopus
    Milk lymphocyte profile and macrophage functions: new insights into the immunity of the mammary gland in quarters infected with Corynebacterium bovis
    (2021) SILVA, Vitoria M.; SOUZA, Marina T.; BLAGITZ, Maiara G.; SOUZA, Fernando N.; BATISTA, Camila F.; ALVES, Alexandre J.; FERNANDES, Artur C. C.; SANCHEZ, Eduardo M. R.; ORDINOLA-RAMIREZ, Carla M.; COSTA, Luciana da; LIBERA, Alice M. M. P. Della
    Backgrounds The present study explored the viability of bovine milk macrophages, their intracellular production of reactive oxygen and nitrogen species (RONS), and their phagocytosis of Staphylococcus aureus, as well as the profile of lymphocytes, from healthy udder quarters and udder quarters infected by Corynebacterium bovis. The study included 28 healthy udder quarters from 12 dairy cows and 20 udder quarters infected by C. bovis from 10 dairy cows. The percentages of macrophages and lymphocytes were identified by flow cytometry using monoclonal antibodies. Macrophage viability, RONS production, and S. aureus phagocytosis were evaluated by flow cytometry. Results Milk samples from quarters infected with C. bovis showed a lower percentage of macrophages but an increased number of milk macrophages per mL and a higher percentage of macrophages that produced intracellular RONS and phagocytosed S. aureus. No effect of C. bovis infection on macrophage viability was found. Udder quarters infected by C. bovis showed a higher percentage of T cells and CD4(+) T lymphocytes, but no effect was found on the percentage of CD8(+) CD4(-) T, CD8(-) CD4(-) T, or B lymphocytes. Conclusions Thus, our results corroborate, at least in part, the finding that intramammary infections by C. bovis may offer protection against intramammary infections by major pathogens.
  • article 6 Citação(ões) na Scopus
    Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows
    (2021) LIMA, Ewerton de Souza; BLAGITZ, Maiara Garcia; BATISTA, Camila Freitas; ALVES, Alexandre Jose; FERNANDES, Artur Cezar de Carvalho; SANCHEZ, Eduardo Milton Ramos; TORRES, Hugo Frias; DINIZ, Soraia Araujo; SILVA, Marcos Xavier; LIBERA, Alice Maria Melville Paiva Della; SOUZA, Fernando Nogueira de
    The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production of reactive oxygen and nitrogen species (RONS) by macrophages in milk samples from dairy cows naturally infected with BLV with or without persistent lymphocytosis (PL). No effect of BLV infection in the overall number of macrophages per milliliter and in the percentage of viable macrophages among overall milk viable cells was found. Furthermore, BLV-infected dairy cows had a higher frequency of viable milk macrophages, while healthy animals had a tendency toward a higher percentage of apoptotic milk macrophages. The percentage of milk macrophages that phagocytosed Staphylococcus aureus in seronegative animals was higher than that in BLV-infected dairy cows. No effect of BLV infection on the intracellular RONS production and the intensity of phagocytosis by milk macrophages was observed. Thus, this study provides new insights into the implications of BLV infections in the bovine mammary gland.
  • article 2 Citação(ões) na Scopus
    Memory CD4+ and CD8+ T lymphocyte proliferation in vaccinated dairy cows with different histories of Staphylococcus aureus mastitis
    (2022) SOARES, Thais C. S.; SANTOS, Kamila R.; LIMA, Daniel M.; MAIA, Raysa Brenda M.; RAMOS-SANCHEZ, Eduardo M.; REIS, Luiza C.; GIDLUND, Magnus; CUNHA, Adriano F. da; ORDINOLA-RAMIREZ, Carla M.; CERQUEIRA, Monica M. O. P.; HEINEMANN, Marcos B.; LIBERA, Alice M. M. P. Della; GOTO, Hiro; SOUZA, Fernando N.
    Staphylococcus aureus mastitis constitutes a serious threat to dairy cows. The reasons why available vaccines are not fully effective remain poorly understood; thus, in the present study, we investigated CD4(+) and CD8(+) T lymphocyte proliferation in dairy cows vaccinated with a polyvalent mastitis vaccine that had distinct precedent Staphylococcus aureus mastitis. We studied 17 S. aureus-infected dairy cows (11 vaccinated and six unvaccinated) and eight vaccinated healthy dairy cows with no previous S. aureus mastitis infections. Flow cytometry was used to assess lymphocyte proliferation using an anti-Ki67 antibody, and monoclonal antibodies were used to identify T cell subsets. S. aureus-infected cows exhibited reduced overall lymphocyte proliferation, including CD4(+) T lymphocyte proliferation, and memory lymphocyte proliferation in response to S. aureus isolate stimulus. Immunization did not influence the expansion of blood lymphocyte populations. Furthermore, CD8(+) T cells, memory CD8(+) T lymphocytes, and effector memory CD8(+) T lymphocytes displayed reduced proliferation 21 days after the third vaccine dose compared with before vaccination at time zero. The present data demonstrates an overall negative regulation of the T-cell response suggesting its detrimental impact leading to the persistence of S. aureus intramammary infections. Furthermore, the lack of vaccination effect on T-cell mediated immunity (e.g., proliferation) may be related to poor vaccine efficacy.