EDUARDO MILTON RAMOS SANCHEZ
Projetos de Pesquisa
Unidades Organizacionais
LIM/38 - Laboratório de Epidemiologia e Imunobiologia, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Recombinant Leishmania infantum Heat Shock Protein 83 for the Serodiagnosis of Cutaneous, Mucosal, and Visceral Leishmaniases(2014) CELESTE, Beatriz Julieta; SANCHEZ, Maria Carmen Arroyo; RAMOS-SANCHEZ, Eduardo Milton; CASTRO, Luiz Guilherme M.; COSTA, Francisco Assis Lima; GOTO, HiroRoutine serological diagnoses for leishmaniases, except in visceral cases, are performed using whole-parasite antigens. We used enzyme-linked immunosorbent assay (ELISA) to evaluate the performance of Leishmania infantum rHsp83 compared with L. major-like total promastigote antigen in the diagnosis of cutaneous (CL), mucosal (ML), and visceral leishmaniases (VL). ELISA-rHsp83 was significantly more sensitive than ELISA-L. major-like when considering either CL/ML (P = 0.041) or all leishmaniasis patients (P = 0.013). When samples from other infectious disease patients were evaluated for cross-reactivity, ELISA-rHsp83 was more specific than ELISA-L. major-like, specifically for Chagas disease samples (P < 0.001). We also evaluated the anti-rHsp83 antibody titers months after treatment and observed no significant difference in ML (P = 0.607) or CL (P = 0.205). We recommend ELISA-L. infantum-rHsp83 as a routine confirmatory serological assay for the diagnosis of Leishmania infection because of the high sensitivity, the specificity, and the insignificant cross-reactivity with other infectious diseases.
conferenceObject Analysis of global gene expression of Leishmania infantum under Insulin-like growth factor I stimulus(2015) RAMOS-SANCHEZ, E. M.; NERLAND, A. H.; GOTO, H.conferenceObject EFFECTS OF INSULIN-LIKE GROWTH FACTOR-I AND IL-4 ON LEISHMANIA (L.) INFANTUM-INFECTED HUMAN MACROPHAGES(2018) GOTO, Hiro; SEVILLANO, Orlando; REIS, Luiza; RAMOS-SANCHEZ, EduardoconferenceObject Th2-skewed response depends on Insulin-like growth factor I in Leishmania (Leishmania) major-infection(2015) REIS, L. C.; RAMOS-SANCHEZ, E. M.; GOTO, H.conferenceObject ANTIBODIES TO PLASMODIUM VIVAX MSP1-19 RECOMBINANT ANTIGEN IN BLOOD DONORS FROM SAO PAULO BLOOD BANK(2017) SANCHEZ, Maria Carmen; ROCHA, Mussya Cisotto; MONTENEGRO, Catarina; FUJIMORI, Mahyumi; SANTI, Silvia Maria Di; SANCHEZ, Arianni Rondelli; RAMOS-SANCHEZ, Eduardo Milton; MENDRONE JUNIOR, Alfredo; LEVI, Jose EduardoconferenceObject INSULIN-LIKE GROWTH FACTOR-I AS EFFECTOR ELEMENT OF IL-4 EFFECT LEADING TO SUSCEPTIBILITY TO LEISHMANIA MAJOR INFECTION(2018) GOTO, Hiro; REIS, Luiza; RAMOS-SANCHEZ, Eduardo; PETITTO-ASSIS, Fabricio; NERLAND, Audun; HERNANDEZ-VALLADARES, Maria; SELHEIM, Frode; FLOETER-WINTER, Lucile