TABATA MARUYAMA DOS SANTOS
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
- Effects of environmental exposure to iron powder in an elastase mice model(2022) GALLI, T. Tafarel; CAMPOS, E. C.; SANTOS, T. M.; FUKUZAKI, S.; CAMARGO, L. N.; BEZERRA, S. K. M.; HAMAGUCHI, S. S. S.; SILVA, F. J. A. Da; SARAIVA-ROMANHOLO, B. M.; OLIVO, C. R.; PRADO, C. M.; LOPES, F. Degobbi Tenorio Quirino Dos Santos; LEICK, E. A.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; LOTUFO, P. A.; RIGHETTI, R. F.; TIBERIO, I. F. L. C.
conferenceObject Cholinergic System in the Exposure of Iron Particles in an Experimental Model of Chronic Allergic Inflammation(2023) TIBERIO, I. C.; SANTOS, T. M.; FUKUZAKI, S.; CAMPOS, E.; GALLI, T. T.; SILVA, L. L. S.; BARBOSA, J. A. S.; JOAO, J. M. L. G.; CAMARGO, L. D.; SARAIVA-ROMANHOLO, B. M.; CIRILLO, J. V. O.; BEZERRA, S. K. M.; PRADO, C. M.; MARTINS, M. A.; RIGHETTI, R. F.; LOPES, F. D.; LEICK, E. A.; SILVA, F. J. A.; REZENDE, B. G.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; LOTUFO, P. A.conferenceObject Anti-IL-17 Treatment Modulates Pulmonary Responses in Model of Asthma-COPD Overlap(2022) CAMARGO, L.; SANTOS, T.; FUKUZAKI, S.; MARTINS, N.; ALMEIDA, F.; LOURENCO, J.; BEZERRA, S.; SARAIVA-ROMANHOLO, B.; PRADO, C.; LOPES, F.; LEICK, E.; RIGHETTI, R.; TIBERIO, I.conferenceObject Preventive Effects of Inhibition of Thymic Stromal Lymphopoietin on Bronchial Hyperresponsiveness and Inflammatory Cellular Pattern in an Experimental Model of Chronic Allergic Pulmonary Inflammation Exacerbated by Lipopolysaccharide(2023) GRENCHESKI, E. A.; LOPES, B. Q. S.; CRUZ, E. L.; RIGHETTI, R. F.; SANTOS, T. M.; CAMARGO, L. D.; ALMEIDA, F.; SARAIVA-ROMANHOLO, B. M.; PRADO, C. M.; BEZERRA, S. K. M.; PIGATI, P. A.; LOPES, F. D.; LEICK, E. A.; TIBERIO, I. F. L. C.conferenceObject Particulate Matter Induces Lung Inflammation in Healthy Mice and Exacerbates Experimental Asthma Model Animals(2023) TIBERIO, I. C.; CAMPOS, E. C.; GALLI, T. T.; SANTOS, T. M.; FUKUZAKI, S.; CAMARGO, L. D.; HAMAGUCHI, S. S. S.; BEZERRA, S. K. M.; SILVA, F. J. A. da; REZENDE, B. G. de; LOPES, F. D. T. Q.; OLIVO, C. R.; SARAIVA-ROMANHOLO, B. M.; PRADO, C. M.; LEICK, E. A.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; RIGHETTI, R. F.; LOTUFO, A.conferenceObject Effects of Exposure to Environmental Pollution From Iron Ore Pellets on Elastase-induced Lung Inflammation: Evaluation of the Effect of Reduced Acetylcholine Transporter Expression in Mice(2023) TIBERIO, I. C.; FUKUZAKI, S.; SANTOS, T. M.; CAMPOS, E. C.; SILVA, L. L. S.; BARBOSA, J. A. S.; JOAO, J. M. L. G.; CAMARGO, L. N.; BEZERRA, S. K. M.; CIRILLO, J. V. O.; PRADO, C. M.; SARAIVA-ROMANHOLO, B. M.; LEICK, E. A.; LOPES, F. D. T. Q.; RIGHETTI, R. F.; SILVA, F. J. A.; BENSENOR, I. J. M.; BOUROTTE, C. L. M.; MARTINS, M. A.; LOTUFO, P. A.- Modulating asthma-COPD overlap responses with IL-17 inhibition(2023) CAMARGO, Leandro do Nascimento; RIGHETTI, Renato Fraga; ALMEIDA, Francine Maria de; SANTOS, Tabata Maruyama dos; FUKUZAKI, Silvia; MARTINS, Nilo Arthur Bezerra; BARBEIRO, Miguel Cantadori; SARAIVA-ROMANHOLO, Beatriz Mangueira; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; LEICK, Edna Aparecida; PRADO, Carla Maximo; TIBERIO, Iolanda de Fatima Lopes CalvoBackground: IL-17 is a modulator of the inflammatory response and is implicated in lung remodeling in both asthma and chronic obstructive pulmonary disease (COPD). Well as and probably in patients with asthma-COPD overlap (ACO).Methods: In this study, we evaluated the response of the airways and alveolar septa to anti-IL-17 treatment in an ACO model. Fifty-six male BALB/c mice were sensitized with ovalbumin (OVA group), received porcine pancreatic elastase (PPE group), or both (ACO group). Mice were then treated with either anti-IL-17 monoclonal antibody or saline. We evaluated hyperresponsiveness, bronchoalveolar lavage fluid (BALF) cell counts, and mean alveolar diameter. We quantified inflammatory, response, extracellular matrix remodeling, oxidative stress markers, and signaling pathway markers.Results: Anti-IL-17 treatment in the ACO anti-IL-17 group reduced the maximum response of respiratory system Rrs, Ers, Raw, Gtis, this when compared to the ACO group (p<0.05). There was a reduction in the total number of inflammatory cells, neutrophils, and macrophages in the BALF in the ACO anti-IL-17 group compared to the ACO group (p<0.05). There was attenuated dendritic cells, CD4+, CD8+, FOXP3, IL-1 beta, IL-2, IL-6, IL-13, IL-17, IL-33 in ACO anti-IL-17 group in airway and alveolar septum compared to the ACO group (p<0.05). We observed a reduction of MMP-9, MMP-12, TIMP-1, TGF-beta, collagen type I in ACO anti-IL-17 group in airway and alveolar septum compared to the ACO group (p < 0.05). We also observed a reduction of iNOS and 8-iso-PGF2 alpha in the airways and in the alveolar septum was reduced in the ACO anti-IL-17group compared to the ACO group (p < 0.05). Regarding the signaling pathways, NF-kB, ROCK-1, and ROCK-2 in the airway and alveolar septum were attenuated in the ACO anti-IL-17 group when compared to the ACO group (p<0.05).Conclusions: Our results suggest that inhibiting IL-17 modulates cell-associated cytokine production in lung tissue, extracellular matrix remodeling, and oxidative stress in ACO through the modulation of NF-kB and FOXP3.
- Effects of plant protease inhibitors (Pep-3-EcTI, Pep-BbKI, and Pep-BrTI) versus corticosteroids on inflammation, remodeling, and oxidative stress in an asthma-COPD (ACO) model(2024) JOAO, Juliana Morelli Lopes Goncalves; BARBOSA, Jessica Anastacia Silva; SILVA, Luana Laura Sales da; FUKUZAKI, Silvia; CAMPOS, Elaine Cristina de; CAMARGO, Leandro do Nascimento; SANTOS, Tabata Maruyama dos; BEZERRA, Suellen Karoline Moreira; ALMEIDA, Francine Maria de; SARAIVA-ROMANHOLO, Beatriz Mangueira; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; BONTURI, Camila Ramalho; RIGHETTI, Renato Fraga; OLIVA, Maria Luiza Vilela; TIBERIO, Iolanda de Fatima Lopes Calvo; LEICK, Edna AparecidaThe peptide derived from E. contortisiliquum trypsin inhibitor (Pep-3-EcTI), peptide derived from kallikrein inhibitor isolated from B. bauhinioides (Pep-BbKI), and B. rufa peptide modified from B. bauhinioides (Pep-BrTI) peptides exhibit anti-inflammatory and antioxidant activities, suggesting their potential for treating asthma-chronic obstructive pulmonary disease (COPD) overlap (ACO). We compared the effects of these peptides with dexamethasone (DX) treatment in an ACO model. In this study, 11 groups of male BALB/c mice were pre-treated under different conditions, including sensitization with intraperitoneal injection and inhalation of ovalbumin (OVA), intratracheal instillation of porcine pancreatic elastase (ELA), sensitization with intraperitoneal injection, and various combinations of peptide treatments with Pep-3-EcTI, Pep-BbKI, Pep-BrTI, dexamethasone, and non-treated controls (SAL-saline). Respiratory system resistance, airway resistance, lung tissue resistance, exhaled nitric oxide, linear mean intercept, immune cell counts in the bronchoalveolar lavage fluid, cytokine expression, extracellular matrix remodeling, and oxidative stress in the airways and alveolar septa were evaluated on day 28. Results showed increased respiratory parameters, inflammatory markers, and tissue remodeling in the ACO group compared to controls. Treatment with the peptides or DX attenuated or reversed these responses, with the peptides showing effectiveness in controlling hyperresponsiveness, inflammation, remodeling, and oxidative stress markers. These peptides demonstrated an efficacy comparable to that of corticosteroids in the ACO model. However, this study highlights the need for further research to assess their safety, mechanisms of action, and potential translation to clinical studies before considering these peptides for human use.