RENATO FRAGA RIGHETTI
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
27 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 27
- Treatment with proteinase inhibitor from Enterolobium contortisiliquum in mice with asthma-COPD(2020) BARBOSA, Jessica Anastacia Silva; SILVA, Luana Laura Sales Da; JOAO, Juliana Morelli Lopes Goncalves; SANTOS, Tabata Maruyama Dos; CAMARGO, Leandro Nascimento; CAMPOS, Elaine Cristina De; GALLI, Thiago Tafarel; SARAIVA-ROMANHOLO, Beatriz Mangueira; BEZERRA, Suellen Karoline Moreira; HAMAGUCHI, Sara Sumie Sobral; LEICK, Edna Aparecida; PRADO, Carla Maximo; MARTINS, Milton De Arruda; OLIVO, Maria Luiza Vilela; RIGHETTI, Renato Fraga; TIBERIO, Iolanda De Fatima Lopes Calvo
- Anti-IL17 treatment control responses in lung injury induced by elastase(2018) FUKUZAKI, Silvia; GARRIDO, Aurelio C.; RIGHETTI, Renato F.; SANTOS, Tabata M.; CAMARGO, Leandro N.; ARISTOTELES, Luciana R. C. R. B.; SOUZA, Flavia C. R.; SARAIVA-ROMANHOLO, Beatriz M.; LEICK, Edna A.; PRADO, Carla M.; MARTINS, Milton A.; TIBERIO, Iolanda F. L. C.
- iNOS Inhibition Reduces Lung Mechanical Alterations and Remodeling Induced by Particulate Matter in Mice(2019) PRADO, Carla Maximo; RIGHETTI, Renato Fraga; LOPES, Fernanda Degobbi Tenorio Quirino dos Santos; LEICK, Edna Aparecida; ARANTES-COSTA, Fernanda Magalhaes; ALMEIDA, Francine Maria de; SALDIVA, Paulo Hilario Nascimento; MAUAD, Thais; TIBERIO, Iolanda de Fatima Lopes Calvo; MARTINS, Milton de ArrudaBackground. The epidemiologic association between pulmonary exposure to ambient particulate matter (PM) and acute lung damage is well known. However, the mechanism involved in the effects of repeated exposures of PM in the lung injury is poorly documented. This study tested the hypotheses that chronic nasal instillation of residual oil fly ash (ROFA) induced not only distal lung and airway inflammation but also remodeling. In addition, we evaluated the effects of inducible nitric oxide inhibition in these responses. For this purpose, airway and lung parenchyma were evaluated by quantitative analysis of collagen and elastic fibers, immunohistochemistry for macrophages, neutrophils, inducible nitric oxide synthase (iNOS), neuronal nitric oxide synthase (nNOS), and alveolar septa 8-iso prostaglandin F2 (8-iso-PGF-2) detection. Anesthetized in vivo (airway resistance, elastance, H, G, and Raw) respiratory mechanics were also analyzed. C57BL6 mice received daily 60ul of ROFA (intranasal) for five (ROFA-5d) or fifteen days (ROFA-15d). Controls have received saline (SAL). Part of the animals has received 1400W (SAL+1400W and ROFA-15d+1400W), an iNOS inhibitor, for four days before the end of the protocol. A marked neutrophil and macrophage infiltration and an increase in the iNOS, nNOS, and 8-iso-PGF2 expression was observed in peribronchiolar and alveolar wall both in ROFA-5d and in ROFA-15d groups. There was an increment of the collagen and elastic fibers in alveolar and airway walls in ROFA-15d group. The iNOS inhibition reduced all alterations induced by ROFA, except for the 8-iso-PGF2 expression. In conclusion, repeated particulate matter exposures induce extracellular matrix remodeling of airway and alveolar walls, which could contribute to the pulmonary mechanical changes observed. The mechanism involved is, at least, dependent on the inducible nitric oxide activation.
conferenceObject Rho Kinase Inhibition Associated To Corticosteroid Treatment Decreases Th1/th2 Responses, Nfkappa B, Mmp-9 And Timp-1 On Airways And Distal Lung Tissue In Animals With Chronic Allergic Inflammation(2013) PIGATI, P. A. Da Silva; RIGHETTI, R. F.; POSSA, S. S.; RODRIGUES, A. P. D.; XISTO, D. G.; LEICK, E. A.; PRADO, C. M.; MARTINS, M. A.; ROCCO, P. R. M.; TIBERIO, I. F. L. C.conferenceObject Effects Of Chronic Treatment With Cape, A Specific Nf Kappa-B Inhibitor, Compared To Rho Quinase Inhibitor Or Corticosteroid On Airway Hyperresponsiveness In Mice With Chronic Allergic Pulmonary Inflammation(2016) TIBERIO, I. F.; SOUZA, F. C. R.; SANTOS, T. M.; ARISTOTELES, L. R.; RIGHETTI, R. F.; SARAIVA-ROMANHOLO, B.; PRADO, C. M.; COSTA, F. M. A.; LEICK, E. A.; MARTINS, M. A.conferenceObject Effect Of Proteinase Inhibitor Of Plant Origin Enterolobium Contortisiliquum Trypsin Inhibitor In An Experimental Model Of Chronic Allergic Pulmonary Inflammation(2015) RODRIGUES, A. P. D.; SANTOS, A. S. A. Dos; RIGHETTI, R. F.; ARANTES-COSTA, F. M.; SARAIVA-ROMANHOLO, B. M.; NOGUEIRA-FILHO, G. G.; BRITO, M. V. De; PRADO, C. M.; LEICK, E. A.; MARTINS, M. D. A.; OLIVA, M. V.; TIBERIO, I. C. L.- Inflammation and remodeling modulated by anti IL17 in model of lung injury induced by elastase in mice(2019) LEICK, Edna A.; FUKUZAKI, Silvia; RIGHETTI, Renato F.; SANTOS, Tabata M.; CAMARGO, Leandro N.; GARRIDO, Aurelio C.; ARISTOTELES, Luciana R. C. R. B.; SOUZA, Flavia C. R.; SARAIVA-ROMANHOLO, Beatriz M.; PRADO, Carla M.; MARTINS, Milton A.; TIBERIO, Iolanda F. L. C.
- Y-27632 is associated with corticosteroid-potentiated control of pulmonary remodeling and inflammation in guinea pigs with chronic allergic inflammation(2015) PIGATI, Patricia Angeli; RIGHETTI, Renato Fraga; POSSA, Samantha Souza; ROMANHOLO, Beatriz Saraiva; RODRIGUES, Adriana Palmeira Dias; SANTOS, Anelize Sartori Alves dos; XISTO, Debora Gonalves; ANTUNES, Mariana Alves; PRADO, Carla Maximo; LEICK, Edna Aparecida; MARTINS, Milton de Arruda; ROCCO, Patrcia Rieken Macedo; TIBERIO, Iolanda de Fatima Lopes CalvoBackground: Previously, we showed that treatment with the Rho-kinase inhibitor Y-27632 was able to control airway responsiveness, inflammation, remodeling, and oxidative stress in an animal model of asthma, suggesting that this drug is beneficial in asthma. However, studies evaluating the effects of these inhibitors in conjunction with corticosteroids on chronic pulmonary inflammation have not been conducted. Therefore, we evaluated the effects of treatment with the Rho-kinase inhibitor Y-27632, with or without concurrent dexamethasone treatment, on airway and lung tissue mechanical responses, inflammation, extracellular matrix remodeling, and oxidative stress in guinea pigs with chronic allergic inflammation. Methods: The guinea pigs were subjected to seven ovalbumin or saline inhalation exposures. Treatment with Y-27632 (1 mM) and dexamethasone (2 mg/kg) started at the fifth inhalation. Seventy-two hours after the seventh inhalation, the pulmonary mechanics were evaluated and exhaled nitric oxide (E-NO) levels were determined. The lungs were removed and histological analysis was performed using morphometry. Results: The treatment of guinea pigs with the Rho-kinase inhibitor and dexamethasone (ORC group) decreased ENO, the maximal mechanical responses after antigen challenge, inflammation, extracellular matrix remodeling and oxidative stress in the lungs. This therapeutic strategy reduced the levels of collagen and IFN-gamma in the airway walls, as well as IL-2, IFN-gamma, 8-iso-PGF2 alpha and NF-kappa B in the distal parenchyma, when compared to isolated treatment with corticosteroid or Rho-kinase inhibitor (P < 0.05) and reduced the number of TIMP-1-positive cells and eosinophils in the alveolar septa compared to corticosteroid-treated animals (P < 0.05). The combined treatment with the Rho-kinase inhibitor and the corticosteroid provided maximal control over the remodeling response and inflammation in the airways and parenchyma. Conclusions: Rho-kinase inhibition, alone or in combination with corticosteroids, can be considered a future pharmacological tool for the control of asthma.
conferenceObject Cholinergic System in the Exposure of Iron Particles in an Experimental Model of Chronic Allergic Inflammation(2023) TIBERIO, I. C.; SANTOS, T. M.; FUKUZAKI, S.; CAMPOS, E.; GALLI, T. T.; SILVA, L. L. S.; BARBOSA, J. A. S.; JOAO, J. M. L. G.; CAMARGO, L. D.; SARAIVA-ROMANHOLO, B. M.; CIRILLO, J. V. O.; BEZERRA, S. K. M.; PRADO, C. M.; MARTINS, M. A.; RIGHETTI, R. F.; LOPES, F. D.; LEICK, E. A.; SILVA, F. J. A.; REZENDE, B. G.; BOUROTTE, C. L. M.; BENSENOR, I. J. M.; LOTUFO, P. A.- Effects of anti-IL-17 on inflammation and oxidative stress in an ASTHMA-COPD overlap syndrome (ACO) model(2021) CAMARGO, Leandro D. N.; MARTINS, Nilo; ALMEIDA, Francine M. D.; LOURENCO, Juliana; BEZERRA, Suellen; SANTOS, Tabata M. D.; FUKUZAKI, Silvia; LOPES, Fernanda D. T. Q. D. S.; MARTIN, Milton M.; LEICK, Edna A.; RIGHETTI, Renato F.; PRADO, Carla M.; TIBERIO, Iolanda D. F. L. C.
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