RENATO FRAGA RIGHETTI
Projetos de Pesquisa
Unidades Organizacionais
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
3 resultados
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conferenceObject Effect Of Proteinase Inhibitor Of Plant Origin Enterolobium Contortisiliquum Trypsin Inhibitor In An Experimental Model Of Chronic Allergic Pulmonary Inflammation(2015) RODRIGUES, A. P. D.; SANTOS, A. S. A. Dos; RIGHETTI, R. F.; ARANTES-COSTA, F. M.; SARAIVA-ROMANHOLO, B. M.; NOGUEIRA-FILHO, G. G.; BRITO, M. V. De; PRADO, C. M.; LEICK, E. A.; MARTINS, M. D. A.; OLIVA, M. V.; TIBERIO, I. C. L.- Y-27632 is associated with corticosteroid-potentiated control of pulmonary remodeling and inflammation in guinea pigs with chronic allergic inflammation(2015) PIGATI, Patricia Angeli; RIGHETTI, Renato Fraga; POSSA, Samantha Souza; ROMANHOLO, Beatriz Saraiva; RODRIGUES, Adriana Palmeira Dias; SANTOS, Anelize Sartori Alves dos; XISTO, Debora Gonalves; ANTUNES, Mariana Alves; PRADO, Carla Maximo; LEICK, Edna Aparecida; MARTINS, Milton de Arruda; ROCCO, Patrcia Rieken Macedo; TIBERIO, Iolanda de Fatima Lopes CalvoBackground: Previously, we showed that treatment with the Rho-kinase inhibitor Y-27632 was able to control airway responsiveness, inflammation, remodeling, and oxidative stress in an animal model of asthma, suggesting that this drug is beneficial in asthma. However, studies evaluating the effects of these inhibitors in conjunction with corticosteroids on chronic pulmonary inflammation have not been conducted. Therefore, we evaluated the effects of treatment with the Rho-kinase inhibitor Y-27632, with or without concurrent dexamethasone treatment, on airway and lung tissue mechanical responses, inflammation, extracellular matrix remodeling, and oxidative stress in guinea pigs with chronic allergic inflammation. Methods: The guinea pigs were subjected to seven ovalbumin or saline inhalation exposures. Treatment with Y-27632 (1 mM) and dexamethasone (2 mg/kg) started at the fifth inhalation. Seventy-two hours after the seventh inhalation, the pulmonary mechanics were evaluated and exhaled nitric oxide (E-NO) levels were determined. The lungs were removed and histological analysis was performed using morphometry. Results: The treatment of guinea pigs with the Rho-kinase inhibitor and dexamethasone (ORC group) decreased ENO, the maximal mechanical responses after antigen challenge, inflammation, extracellular matrix remodeling and oxidative stress in the lungs. This therapeutic strategy reduced the levels of collagen and IFN-gamma in the airway walls, as well as IL-2, IFN-gamma, 8-iso-PGF2 alpha and NF-kappa B in the distal parenchyma, when compared to isolated treatment with corticosteroid or Rho-kinase inhibitor (P < 0.05) and reduced the number of TIMP-1-positive cells and eosinophils in the alveolar septa compared to corticosteroid-treated animals (P < 0.05). The combined treatment with the Rho-kinase inhibitor and the corticosteroid provided maximal control over the remodeling response and inflammation in the airways and parenchyma. Conclusions: Rho-kinase inhibition, alone or in combination with corticosteroids, can be considered a future pharmacological tool for the control of asthma.
conferenceObject Effect Of Proteinase Inhibitor Of Plant Origin Crataeva Tapia Bark Lectin In An Experimental Model Of Chronic Allergic Pulmonary Inflammation(2015) SANTOS, A. S. A.; RODRIGUES, A. P. D.; ARANTES-COSTA, F. M.; SARAIVA-ROMANHOLO, B. M.; NOGUEIRA-FILHO, G. G.; BRITO, M. V.; RIGHETTI, R. F.; PRADO, C. M.; LEICK, E. A.; MARTINS, M. D. A.; OLIVA, M. V.; TIBERIO, I. C. L.