DOMINGOS HIROSHI TSUJI

(Fonte: Lattes)
Índice h a partir de 2011
11
Lattes
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Departamento de Otorrinolaringologia e Oftalmologia, Faculdade de Medicina - Docente
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/32 - Laboratório de Otorrinolaringologia, Hospital das Clínicas, Faculdade de Medicina

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  • article 6 Citação(ões) na Scopus
    Effect of Fibrin Glue on Collagen Deposition After Autologous Fascia Grafting in Rabbit Vocal Folds
    (2011) SCAPINI, Fabricio; SILVA, Luiz Fernando Ferraz da; TSUJI, Domingos Hiroshi; DOLHNIKOFF, Marisa; SENNES, Luiz Ubirajara
    Objectives: Fibrin glue (FG) is a reaction product of fibrinogen and thrombin that forms a fibrin clot responsible for tissue adhesion. However, FG and its components may interfere with wound healing by interacting with cytokines such as transforming growth factor-beta (TGF-beta). The objective of this study was to investigate the effect of FG on collagen deposition after fascia grafting in the vocal folds of rabbits. Methods: Eighteen rabbits underwent autologous fascia grafting in both vocal folds, and the left side was fixed with FG. Each animal was painlessly sacrificed after 7,30, or 90 clays. The larynx was removed, and the vocal folds were prepared for histomorphometric analysis by picrosirius red staining to evaluate collagen deposition around the graft. Results: There was a significant increase in collagen density around the grafts at 90 days in the vocal folds that were fixed with FG (p = 0.0102) compared with the control vocal folds. Conclusions: Application of FG altered collagen deposition around the fascia grafts, leading to significantly increased collagen density after 90 days. Differences found in the composition of the extracellular matrix in later stages of the healing process are a result of changes that occur in the beginning of this process. Therapeutic interventions, such as the use of FG and/or its components, performed in the early stages of wound healing may interfere with the complex interactions of fibroblasts, inflammatory cells, and cytokines (especially TGF-beta), thereby modulating the healing process.
  • article 12 Citação(ões) na Scopus
    Collagen Type I, Collagen Type III, and Versican in Vocal Fold Lamina Propria
    (2011) BUEHLER, Rogerio B.; SENNES, Luiz U.; TSUJI, Domingos H.; MAUAD, Thais; SILVA, Luiz Ferraz da; SALDIVA, Paulo N.
    Objective: To analyze the distributions of collagen type I, collagen type III, and versican in the lamina propria of the human vocal fold. Design: Cross-sectional analysis of cadaveric vocal folds of adult human larynges. Setting: Academic tertiary referral center. Subjects: Larynges harvested at autopsy from 10 adult men and 10 adult women. Main Outcome Measures: Immunohistochemical reactions were performed using antihuman monoclonal antibodies to analyze the expression of collagen type I, collagen type III, and versican. Results: Collagen type I density was lower in the intermediate layer compared with the superficial and deep layers of vocal folds. Collagen type III density was lower in the intermediate layer compared with the deep layer. Versican density was lower in the superficial layer compared with the intermediate and deep layers. Versican density was lower in the lamina propria of women compared with men; this difference was noted in the superficial layer only. There was a positive correlation between collagen type III and versican densities within the lamina propria. Conclusion: Collagen type I, collagen type III, and versican are distributed differently within the lamina propria layers of the adult vocal folds.
  • article 36 Citação(ões) na Scopus
    Voice Analysis of Postlingually Deaf Adults Pre- and Postcochlear Implantation
    (2011) UBRIG, Maysa Tiberio; GOFFI-GOMEZ, Maria Valeria S.; WEBER, Raimar; MENEZES, Marcia H. Moreira; NEMR, Nair Katia; TSUJI, Domingos Hiroshi; TSUJI, Robinson Koji
    Objectives. To ascertain whether cochlear implantation (Cl), without specific vocal rehabilitation, is associated with changes in perceptual and acoustic vocal parameters in adults with severe to profound postlingual deafness. Hypothesis. Merely restoring auditory feedback could allow the individual to make necessary adjustments in vocal pattern. Study Design. Prospective and longitudinal. Methods. The experimental group composed of 40 postlingually deaf adults (20 males and 20 females) with no previous laryngeal or voice disorders. Participants' voices were recorded before CI and 6-9 months after Cl. To check for chance modifications between two evaluations, a control group of 12 postlingually deaf adults, six male and six female, without CI was also evaluated. All sessions composed of the recording of read sentences from Consensus Auditory-Perceptual Evaluation of Voice and sustained vowel /a/. Auditory and acoustic analyses were then conducted. Results. We found a statistically significant reduction in overall severity, strain, loudness, and instability in auditory analysis. In vocal acoustic analysis, we found statistically significant reduction fundamental frequency (F0) values (in male participants) and F0 variability (in both genders). The control group showed no statistically significant changes in most vocal parameters assessed, apart from pitch and F0 (in female participants only). On comparing the interval of variation of results between the experimental and control groups, we found no statistically significant difference in vocal parameters between Cl recipients and nonrecipients, with the exception of F0 variability in male participants. Conclusions. The patients in our sample showed changes in overall severity, strain, loudness, and instability values, and reductions in F0 and its variability. On comparing the variation of results between the groups, we were able to prove in our study that implant recipients postlingually deaf adults (experimental group), without specific vocal rehabilitation, differed from nonrecipients (control group) in loudness and F0 variability sustained vowel /a/ in male participants.