LEANDRO EZIQUIEL DE SOUZA
Índice h a partir de 2011
7
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
conferenceObject IMPACT OF ANGIOTENSIN CONVERTING ENZYME (ACE) GENE LEVELS ON BAROREFLEX IMPAIRMENT AND SYMPATHETIC HYPERACTIVITY AFTER A HIGH FRUCTOSE DIET(2017) MORAES-SILVA, Ivana C.; SOUZA, Leandro E. de; CASARINI, Dulce E.; IRIGOYEN, Maria-ClaudiaconferenceObject Effects of aerobic exercise training on obese female mice: metabolic and autonomic evaluation(2013) SARTORI, Michelle; SOUZA, Leandro Eziquiel de; SOUZA, Pamella Ramona de; SANTOS, Fernando; ANGELIS, Katia De; IRIGOYEN, Maria ClaudiaThe aim of this study was to evaluate the metabolic and autonomic parameters in trained youth obese mice. Female ob/ob mice (4 weeks old) were randomized into sedentary (OS, n=11) or trained (OT, n=8) (treadmill,5 days/w, 60 min/d, during 8 wks) groups. Blood glucose was measured by reagent strips. Blood pressure signals were recorded using a data acquisition system. The OT group had higher exercise capacity compared to the OS group. OT group had reduced body weight, white adipose tissue and blood glucose (45±2g, 6.1±1g and 133±3g mg/dL) compared to OS group (50±2g, 6.1±1g and 169±8 mg/dL). Exercise training improved glucose tolerance in trained group (OS: 27961±6501 vs. OT: 18258±6283 mg/dL/min). Mean arterial pressure and heart rate were similar between groups. The trained group showed increase in heart rate variability compared with sedentary group (OT: 50±12 vs. OS: 33±8ms2). Blood pressure variability and low frequency band were higher in OS group (23 ± 3mmHg2 and 7 ± 1 mmHg2) compared to OT (15 ± 2 mmHg2 and 5 ± 1 mmHg2). Moreover, the spontaneous baroreflex sensitivity, represented by alpha index, was lower in sedentary group (1.2 ± 0.3 ms/mmHg) compared to trained group (1.9 ± 0.2 ms/mmHg). In conclusion, our results suggest that physical training during lifespan can be effective inattenuating the increase in weight and blood glucose and improving blood pressure variability in obese mice.conferenceObject The metabolic mechanisms involved in the heart protection from myocardial infarction induced by diabetes(2012) MALFITANO, Christiane; CARBONARO, M.; SOUZA JUNIOR, A. L. de; ALBA-LOUREIRO, T. C.; SOUZA, L. E.; FIGUEROA, D.; SILVA, K.; CURI, R.; IRIGOYEN, M. C.conferenceObject Interval training program improves cardiac function and physical performance in heart failure mice(2013) ABAD, Cesar Cavinato Cal; MOSTARDA, Cristiano Teixeira; NASCIMENTO, Ademir Manuel; SANTOS, Mirailton Alves dos; SOUZA, Leandro Eziquiel; FIGUEROA, Diego M. T.; SANTOS, Fernando; SOUZA, Pamella Ramona Moraes de; ANGELIS, Katia De; IRIGOYEN, Maria ClaudiaThis study was designed to verify the effect of an eight week interval training program (IT) in systolic and diastolic functions and physical performance in heart failure mice (C57B16, n=24). The animals were divided in three groups: 1) Intact sedentary (C), 2) infarcted sedentary (MI-s); and 3) infarcted trained (MI-i). At the begining (M1) and at the end of IT (M2), time of maximal running performance (Tvmáx) was quantified. The ejection fraction (EF), shortening fraction (SF) and isovolumetric relaxation time (IVRT) were accessed by echocardiography in M2. At 60 days after coronary artery occlusion the IT was started (4min at 80% Vmáx x 4min at 40% Vmáx during 60min). The results are presented in mean and SEM. Myocardial infarction area was not different between groups in both evaluation time (M1vsM2). The EF and SF in MI-s were lower than C (44±3.7vs58±3.0% and 22±2vs31±2%, respectively; p≤0.05) but similar between MI-i and C (56±5vs58±3% and 25±3vs31±2%, respectively). The IVRT was higher (p≤0.05) in MI-s (25.6±4ms) than C (13±1ms) while similar in MI-i (15±0.8ms) and C. At the M1 both MI-s and MI-i Tvmáx decreased in comparison with C (620±16 and 644±28vs840±17s, respectively). At the M2, Tvmáx delta percent was higher in MI-i than MI-s (64±8vs15±6%, respectively; p≤0.05) and C (0.53±3%). The results suggest that IT improves cardiac function and physical performance in heart failure mice.- (Pro)renin receptor expression in myocardial infarction in transgenic mice expressing rat tonin(2018) RIBEIRO, Amanda A.; AMORIM, Rebeca Padrao; PALOMINO, Zaira J.; LIMA, Mercia de Paula; MORAES-SILVA, Ivana Cinthya; SOUZA, Leandro Ezequiel; PESQUERO, Jorge Luiz; IRIGOYEN, Maria Claudia; CASARINI, Dulce E.The (pro)renin receptor [(P)RR] has been implicated as a renin/prorenin receptor, and plays a role in local renin angiotensin system activation. Our goal was to investigate whether a transgenic mouse that expresses rat tonin [TGM'(rTon)] can regulate (P)RR mRNA levels. Control (C) and TGM'(rTon) animals were subdivided into the C sham, C MI, TGM'(rTon) sham, and TGM'(rTon) MI groups. The levels of tonin, (P)RR, and renin were determined using RT-PCR mRNA. Tonin activity as determined by RIE was significantly increased in the TGM'(rTon) sham group as compared to the C sham group in the atrium (AT) and right ventricle (RV), respectively. In most mice, tonin mRNA levels were significantly reduced compared to those in the TGM'(rTon) sham group in the atria. In this structure, the (P)RR mRNA levels were statistically significantly reduced in the TGM'(rTon) sham and TGM'(rTon) MI groups compared to the control groups. However, the (P)RR mRNA values were significantly increased when we compared the TGM'(rTon) MI vs TGM'(rTon) sham groups. In the RV, the renin mRNA levels in the TGM'(rTon) sham group were significantly reduced compared to the C sham group. Tonin overexpression may act in the regulation of (P)RR mRNA levels during MI.
conferenceObject Ventricular disfunction induces morphofunctional changes in adrenal glands(2016) ANDRADE, Thulio Ramos de; SILVA, Juliane; SOUZA, Leandro; JORDAO, Camila; FERRELRA, Tatiane; NEGRAO, Carlos; BRUM, Patricia; IRIGOYEN, Maria Claudia; LACCHINI, Silvia; ALVES, Maria JanieireconferenceObject Simvastatin improves cardiovascular sympathetic modulation and endothelial function of resistance arteries from hypercholesterolemic mice(2012) MORAES-SILVA, Ivana C.; SOUZA, L. E.; ROSSONI, L. V.; IRIGOYEN, M. C.Can simvastatin treatment (S) alter sympathetic cardiovascular control and mesenteric resistance arteries (MRA) relaxation of LDL receptor knock out (L) mice? Male L mice were treated with S (2 mg/kg, i.p., 7 days) or vehicle. C67Bl/6 mice were used as control (C). Total cholesterol (TC), blood pressure (BP) and autonomic modulation were analyzed. MRA rings were studied in an isometric myograph. Concentration-response curves to Ach (0.01nM-30μM) were obtained in rings incubated for 30 min with L-NAME (100 μM), a NOS inhibitor, tetraethylammonium (TEA; 5mM), a K+ channel blocker, or with vehicle. TC was 3x higher and BP was increased (systolic:30%, diastolic:7%) in L vs. C whereas S had no effect. Cardiac autonomic balance, which was 3x higher, and sympathetic modulation to the vessels, 5x higher in L vs. C, were significantly reduced in L+S (17 and 57%, respectively). MRA % of relaxation to Ach was impaired in L (37±2%) vs. C (92±5%) while S restored it to 70±6%. In C MRA, L-NAME and TEA inhibited Ach-induced relaxation in 46±8% and 74±5%, respectively (p<0.05 L-NAME vs. TEA). This inhibition was ~90±2% in L for both drugs (p<0.05 vs. C); while S shifted it to 70±7% of relaxation inhibition (p<0.05 vs. C and L). S partially restored MRA endothelial function in L mainly by modulating NO concomitantly with an improved autonomic cardiovascular control even without changes in systemic BP and lipid levels.conferenceObject Markers of acute cardiovascular inflammation induced by angiotensin II in a murine model(2013) SANTANA, Andre Bento Chaves; SOUZA-OLIVEIRA, Thais; BARAUNA, Valerio; SOUZA, Leandro; IRIGOYEN, Maria Claudia; CAMPOS, Luciene; KRIEGER, Jose Eduardo; LACCHINI, SilviaAngiotensin II has important physiological functions for the homeostasis of the cardiovascular system and may also induce to inflammatory responses. The aim of this study was to evaluate the effetc of subpressor angiotensin II (AngII) on expression of inflammatory markers in cardiac vessels. Were used C57Bl/6J male mice, treated with subpressor dose of AngII (30ng/kg IP), confirmed by carotid catheterization and arterial pressure measure after 10, 30, 60min, and 2 and 6hours of AngII injection (n=5/saline; n=5/AngII). Inflammatory markers were evaluated in cardiac vessels (TGF-beta, IL-6 and iNOS) by western blot, and localized in vessels by immunohistochemistry. Mice were evaluated after 1 and 24 hours to identify acute responses. Results were compared by ANOVA, using p≤0.05 as significant. Blood pressure measurements showed no changes in arterial pressure and heart rate. Protein analysis showed an increase of inflammatory markers in cardiac tissue after 1 hour TGF-beta (84%), IL-6 (90%) and iNOS (70%). However, these markers were unchanged after 24 hours. Immunohistochemistry analysis showed a specific increase in these markers associated to cardiac vessels. The results suggests that, independently on hemodynamic influences, Angiotensin II leads to expression of inflammatory markers in cardiac vessels in a short period, and this may represent a direct pro-inflammatory action mediated by angiotensin II.