FRANCINE MARIA DE ALMEIDA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice (vol 8, 12636, 2018)(2018) GENARO, Isabella Santos de; ALMEIDA, Francine Maria de; HIZUME-KUNZLER, Deborah Camargo; MORIYA, Henrique Takachi; SILVA, Ronaldo Aparecido; CRUZ, Joao Carlos Goncalves; LOPES, Renan Boeira; RIGHETTI, Renato Fraga; VIEIRA, Rodolfo de Paula; SAIKI, Mitiko; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; ARANTES-COSTA, Fernanda Magalhaes; SARAIVA-ROMANHOLO, Beatriz Mangueira
conferenceObject Creatine Supplementation Attenuates Apoptosis and Proliferation of Inflammatory Cells in a Lung Transplantation Model(2018) ALMEIDA, F. M.; BATTOCHIO, A. S.; NAPOLI, J. P.; ALVES, K. A.; BALBIN, G. S.; OLIVEIRA-JUNIOR, M.; MORIYA, H. T.; PEGO-FERNANDES, P.; VIEIRA, R. P.; PAZETTI, R.- Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice(2018) GENARO, Isabella Santos de; ALMEIDA, Francine Maria de; HIZUME-KUNZLER, Deborah Camargo; MORIYA, Henrique Takachi; SILVA, Ronaldo Aparecido; CRUZ, Joao Carlos Goncalves; LOPES, Renan Boeira; RIGHETTI, Renato Fraga; VIEIRA, Rodolfo de Paula; SAIKI, Mitiko; MARTINS, Milton Arruda; TIBERIO, Iolanda de Fatima Lopes Calvo; ARANTES-COSTA, Fernanda Magalhaes; SARAIVA-ROMANHOLO, Beatriz MangueiraWork-exacerbated asthma (WEA) is defined as preexisting asthma that worsens with exposure to irritants [e.g., chlorine (Cl-2) derivatives] in the workplace. The maximum allowable concentration in the workplace of Cl-2 exposure is 3 mg/m(3) (described in OSHA). We investigated in an experimental asthma model in mice the effects of a single exposure to a sodium hypochlorite dose with this allowed chlorine concentration and a tenfold higher dose. Acute chlorine exposure at 3.3 mg/m(3) in the OVA-sensitized group increased eosinophils in the peribronquial infiltrate, cytokine production, nasal mucus production and the number of iNOS positive cells in the distal lung compared to only sensitized mice. The exposure to a higher dose of 33.3 mg/m(3) in the OVA-sensitized group resulted in an increase in respiratory system elastance, in the total and differential numbers of inflammatory cells in bronchoalveolar lavage fluid, IL-4, IL-5, and IL-17 in the lungs, eosinophils in peribronquial infiltrate and mucus content in nasal compared to non-exposed and sensitized animals. In this asthma model, chorine exposures at an allowable dose, contributed to the potentiation of Th2 responses. The functional alterations were associated with increased iNOS and ROCK-2 activation in the distal lung.
- Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation?(2018) ABREU, Marcus da Matta; ALMEIDA, Francine Maria de; SANTOS, Kelli Borges dos; ASSIS, Emilio Augusto Campos Pereira de; HAMADA, Rafael Kenji Fonseca; JATENE, Fabio Biscegli; PEGO-FERNANDES, Paulo Manuel; PAZETTI, Rogerio
- Pre- and postnatal exposure of mice to concentrated urban PM2.5 decreases the number of alveoli and leads to altered lung function at an early stage of life(2018) LOPES, Thais de Barros Mendes; GROTH, Espen E.; VERAS, Mariana; FURUYA, Tatiane K.; COSTA, Natalia de Souza Xavier; RIBEIRO JUNIOR, Gabriel; LOPES, Fernanda Degobbi; ALMEIDA, Francine M. de; CARDOSO, Wellington V.; SALDIVA, Paulo Hilario Nascimento; CHAMMAS, Roger; MAUAD, ThaisGestational exposure to air pollution is associated with negative outcomes in newborns and children. In a previous study, we demonstrated a synergistic negative effect of pre- and postnatal exposure to PM2.5 on lung development in mice. However, the means by which air pollution affects development of the lung have not yet been identified. In this study, we exposed pregnant BALB/c mice and their offspring to concentrated urban PM2.5 (from Sao Paulo, Brazil; target dose 600 mu g/m(3) for 1 h daily). Exposure was started on embryonic day 5.5 (E5.5, time of placental implantation). Lung tissue of fetuses and offspring was submitted to stereological and transcriptomic analyses at E14.5 (pseudoglandular stage of lung development), E18.5 (saccular stage) and P40 (postnatal day 40, alveolarized lung). Additionally, lung function and cellularity of bronchoalveolar lavage (BAL) fluid were studied in offspring animals at P40. Compared to control animals that were exposed to filtered air throughout gestation and postnatal life, PM-exposed mice exhibited higher lung elastance and a lower alveolar number at P40 whilst the total lung volume and cellularity of BAL fluid were not affected. Glandular and saccular structures of fetal lungs were not altered upon gestational exposure; transcriptomic signatures, however, showed changes related to DNA damage and its regulation, inflammation and regulation of cell proliferation. A differential expression was validated at E14.5 for the candidates Sox8, Angptl4 and Gas1. Our data substantiate the in utero biomolecular effect of gestational exposure to air pollution and provide first-time stereological evidence that pre- and early life-postnatal exposure compromise lung development, leading to a reduced number of alveoli and an impairment of lung function in the adult mouse.
conferenceObject Clinical, Functional and Inflammatory Evaluation in Asthmatic Patients After a Simple Short-Term Educational Program(2018) FELIX, S. N.; AGONDI, R. C.; AUN, M. V.; KURKEJAK, A.; AVONA, M. D.; AMORIM, T. S.; LOPES, M. R.; ALMEIDA, F. M.; GENARO, I. S.; BEZERRA, S. K.; GIAVINA-BIANCHI, P.; KALIL, J.; TIBERIO, I. D.; MARTINS, M. D.; SARAIVA-ROMANHOLO, B. M.