FRANCINE MARIA DE ALMEIDA
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/20 - Laboratório de Terapêutica Experimental, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
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- Lodenafil treatment in the monocrotaline model of pulmonary hypertension in rats(2014) POLONIO, Igor Bastos; ACENCIO, Milena Marques Pagliareli; PAZETTI, Rogerio; ALMEIDA, Francine Maria de; SILVA, Brbara Soares da; PEREIRA, Karina Aparecida Bonifacio; SOUZA, RogerioWe assessed the effects of Iodenafil on hemodynamics and inflammation in the rat model of monocrotaline-induced pulmonary hypertension (PH). Thirty male Sprague-Dawley rats were randomly divided into three groups: control; monocrotaline (experimental model); and Iodenafila (experimental model followed by Iodenafil treatment, p.o., 5 mg/kg daily for 28 days) Mean pulmonary artery pressure (mPAP) was obtained by right heart catheterization. We investigated right ventricular hypertrophy (RVH) and IL-1 levels in lung fragments. The number of cases of RVH was significantly higher in the monocrotaline group than in the Iodenafil and control groups, as were mPAP and IL-1 levels. We conclude that Iodenafil can prevent monocrotaline-induced PH, RVH, and inflammation.
- Comparação de dois modelos experimentais de hipertensão pulmonar(2012) POLONIO, Igor Bastos; ACENCIO, Milena Marques Pagliarelli; PAZETTI, Rogerio; ALMEIDA, Francine Maria de; CANZIAN, Mauro; SILVA, Barbara Soares da; PEREIRA, Karina Aparecida Bonifacio; SOUZA, Rogerio deObjective: To compare two models of pulmonary hypertension (monocrotaline and monocrotaline+pneumonectomy) regarding hemodynamic severity, structure of pulmonary arteries, inflammatory markers (IL-1 and PDGF), and 45-day survival. Methods: We used 80 Sprague-Dawley rats in two study protocols: structural analysis; and survival analysis. The rats were divided into four groups: control; monocrotaline (M), pneumonectomy (P), and monocrotaline+pneumonectomy (M+P). In the structural analysis protocol, 40 rats (10/group) were catheterized for the determination of hemodynamic variables, followed by euthanasia for the removal of heart and lung tissue. The right ventricle (RV) was dissected from the interventricular septum (IS), and the ratio between RV weight and the weight of the left ventricle (LV) plus IS (RV/LV+IS) was taken as the index of RV hypertrophy. In lung tissues, we performed histological analyses, as well as using ELISA to determine IL-1 and PDGF levels. In the survival protocol, 40 animals (10/group) were followed for 45 days. Results: The M and M+P rats developed pulmonary hypertension, whereas the control and P rats did not. The RV/LV+IS ratio was significantly higher in M+P rats than in M rats, as well as being significantly higher in M and M+P rats than in control and P rats. There were no significant differences between the M and M+P rats regarding the area of the medial layer of the pulmonary arteries; IL-1 and PDGF levels; or survival. Conclusions: On the basis of our results, we cannot conclude that the monocrotaline+pneumonectomy model is superior to the monocrotaline model.
- Does methylene blue attenuate inflammation in nonischemic lungs after lung transplantation?(2018) ABREU, Marcus da Matta; ALMEIDA, Francine Maria de; SANTOS, Kelli Borges dos; ASSIS, Emilio Augusto Campos Pereira de; HAMADA, Rafael Kenji Fonseca; JATENE, Fabio Biscegli; PEGO-FERNANDES, Paulo Manuel; PAZETTI, Rogerio