REGINA MARIA DE CARVALHO PINTO
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Coração, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
LIM/09 - Laboratório de Pneumologia, Hospital das Clínicas, Faculdade de Medicina
7 resultados
Resultados de Busca
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- Age-associated phenotypic imbalance in TCD4 and TCD8 cell subsets: comparison between healthy aged, smokers, COPD patients and young adults(2022) FERNANDES, Juliana Ruiz; PINTO, Thalyta Nery Carvalho; ARRUDA, Lia Barbara; SILVA, Cibele Cristine Berto Marques da; CARVALHO, Celso Ricardo Fernandes de; PINTO, Regina Maria Carvalho; DUARTE, Alberto Jose da Silva; BENARD, GilBackground COPD is associated with an abnormal lung immune response that leads to tissue damage and remodeling of the lung, but also to systemic effects that compromise immune responses. Cigarette smoking also impacts on innate and adaptative immune responses, exerting dual, pro- and anti-inflammatory effects. Previously, we showed that COPD patients presented accelerated telomere shortening and decreased telomerase activity, while, paradoxically, cigarette-smokers exhibited preserved telomerase activity and slower rate of telomere shortening. Results Here, we evaluated the naive, CM, EM and T-EMRA subsets of TCD4 and TCD8 cells according to the expression of CCR7/CD45RA. We compared age-matched COPD patients, cigarette-smokers without clinical-laboratory evidence of pulmonary compromise, and healthy individuals. They were additionally compared with a group of young adults. For each subset we analysed the expression of markers associated with late differentiation, senescence and exhaustion (CD27/CD28/CD57/KLRG1/PD1). We show that COPD patients presented a drastically reduced naive cells pool, and, paradoxically, increased fractions of naive cells expressing late differentiation, senescence or exhaustion markers, likely impacting on their immunocompetence. Pronounced phenotypic alterations were also evidenced in their three memory T-cell subsets compared with the other aged and young groups, suggesting an also dysfunctional memory pool. Surprisingly, our smokers showed a profile closer to the Healthy aged than COPD patients. They exhibited the usual age-associated shift of naive to EM TCD4 and TCD8 cells, but not to CM or T-EMRA T-cells. Nonetheless, their naive T-cells phenotypes were in general similar to those of the Youngs and Healthy aged, suggesting a rather phenotypically preserved subset, while the memory T-cells exhibited increased proportions of cells with the late-differentiation or senescence/exhaustion markers as in the Healthy aged. Conclusion Our study extends previous findings by showing that COPD patients have cells expressing a full range of late differentiated, senescent or exhausted phenotypes encompassing all TCD4 and TCD8 subsets, consistent with a premature immunosenescence phenotype. Surprisingly, the smokers group's results suggest that moderate to heavy chronic cigarette smoking did not accelerate the pace of immunosenescence as compared with the Healthy aged.
- Postural balance in COPD with obstructive sleep apnea: a cross-sectional study(2022) CENSO, C. Maschio de; PASSINI, V. Vieira; VERRI, B. Aparecida Teodoro Alcantara; CARVALHO-PINTO, R. Maria De; STELMACH, R.; LORENZI-FILHO, G.; XAVIER, R. Fagundes; CARVALHO, C. Ricardo Fernandes
- Celebrating World Asthma Day in Brazil: lessons learned from the pandemic. Can we do better?(2022) PIZZICHINI, Marcia Margaret Menezes; CARVALHO-PINTO, Regina Maria de; PIZZICHINI, Emilio
- Behavior change to increase physical activity reduces sleep disturbances in asthma: A randomized controlled trial(2022) FREITAS, P.; PASSOS, N. F.; CUKIER, A.; CARVALHO-PINTO, R.; CARVALHO, C. R. Fernandes de
- Effect of physical effort on postural balance in COPD and OSA(2022) CENSO, C. Maschio de; PASSINI, V. Vieira; VERRI, B. Aparecida Teodoro Alcantara; CARVALHO-PINTO, R. Maria De; STELMACH, R.; LORENZI-FILHO, G.; XAVIER, R. Fagundes; CARVALHO, C. Ricardo Fernandes
- InspiAir, the mHealth App to monitor asthma symptoms for people with asthma(2022) CARVALHO, C. R. Fernandes de; VELLOSO, L.; CARVALHO-PINTO, R.; YASIRO, R.; FARFAN, C.; JENSEN, G.; FILGUEIRAS, L.
- Characteristics of individuals with moderate to severe asthma who better respond to aerobic training: a cluster analysis(2022) LUNARDI, A.; FREITAS, P.; ENDRIGUE, T.; SILVA, R.; MENDES, F.; FRANCA, A.; CARVALHO-PINTO, R.; CARVALHO, R. Fernandes de