ELEAZAR CHAIB

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Departamento de Gastroenterologia, Faculdade de Medicina - Docente
LIM/37 - Laboratório de Transplante e Cirurgia de Fígado, Hospital das Clínicas, Faculdade de Medicina - Líder

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Autor e Coautores FMUSP-HC Normalizados: ENIO RODRIGUES VASQUES ×

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Agora exibindo 1 - 10 de 10
  • article 19 Citação(ões) na Scopus
    Trisulfate Disaccharide Decreases Calcium Overload and Protects Liver Injury Secondary to Liver Ischemia/Reperfusion
    (2016) VASQUES, Enio Rodrigues; CUNHA, Jose Eduardo Monteiro; COELHO, Ana Maria Mendonca; SAMPIETRE, Sandra N.; PATZINA, Rosely Antunes; ABDO, Emilio Elias; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo Andrade; GODOY, Carlos M. G.; RODRIGUES, Tiago; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.
    Background Ischemia and reperfusion (I/R) causes tissue damage and intracellular calcium levels are a factor of cell death. Sodium calcium exchanger (NCX) regulates calcium extrusion and Trisulfated Disaccharide (TD) acts on NCX decreasing intracellular calcium through the inhibition of the exchange inhibitory peptide (XIP). Objectives The aims of this research are to evaluate TD effects in liver injury secondary to I/R in animals and in vitro action on cytosolic calcium of hepatocytes cultures under calcium overload. Methods Wistar rats submitted to partial liver ischemia were divided in groups: Control: (n = 10): surgical manipulation with no liver ischemia; Saline: (n = 15): rats receiving IV saline before reperfusion; and TD: (n = 15): rats receiving IV TD before reperfusion. Four hours after reperfusion, serum levels of AST, ALT, TNF-alpha, IL-6, and IL-10 were measured. Liver tissue samples were collected for mitochondrial function and malondialdehyde (MDA) content. Pulmonary vascular permeability and histologic parameters of liver were determined. TD effect on cytosolic calcium was evaluated in BRL3A hepatic rat cell cultures stimulated by thapsigargin pre and after treatment with TD. Results AST, ALT, cytokines, liver MDA, mitochondrial dysfunction and hepatic histologic injury scores were less in TD group when compared to Saline Group (p<0.05) with no differences in pulmonary vascular permeability. In culture cells, TD diminished the intracellular calcium raise and prevented the calcium increase pre and after treatment with thapsigargin, respectively. Conclusion TD decreases liver cell damage, preserves mitochondrial function and increases hepatic tolerance to I/R injury by calcium extrusion in Ca2+ overload situations.
  • article 0 Citação(ões) na Scopus
    A new heparin fragment decreases liver ischemia-reperfusion injury
    (2022) VASQUES, Enio R.; FIGUEIRA, Estela R. R.; ROCHA-FILHO, Joel A.; LANCHOTTE, Cinthia; XIMENES, Jorge L. S.; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo A.; RODRIGUES, Tiago; CUNHA, Jose E. M.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz A. C.; GALVAO, Flavio H. F.
  • article 5 Citação(ões) na Scopus
    THE M-RNA, EXPRESSION OF SERCA2 AND NCX1 IN THE PROCESS OF PHARMACOLOGICAL CELL PROTECTION IN EXPERIMENTAL ACUTE PANCREATITIS INDUCED BY TAUROCHOLATE
    (2018) VASQUES, Enio Rodrigues; CUNHA, Jose Eduardo Monteiro; KUBRUSLY, Marcia Saldanha; COELHO, Ana Maria; SANPIETRI, Sandra N.; NADER, Helena B.; TERSARIOL, Ivarne L. S.; LIMA, Marcelo A.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto Carneiro
    Background: Intracellular calcium overload is known to be a precipitating factor of pancreatic cell injury in acute pancreatitis (AP). Intracellular calcium homeostasis depends of Plasmatic Membrane Calcium ATPase (PMCA), Sarcoplasmic Endothelial Reticulum Calcium ATPase 2 (SERCA 2) and the Sodium Calcium Exchanger (NCX1). The antioxidant melatonin (Mel) and Trisulfate Disaccharide (TD) that accelerates NCX1 action could reduce the cell damage determined by the AP. Aim: To evaluate m-RNA expressions of SERCA2 and NCX1 in acute pancreatitis induced by sodium taurocholate in Wistar rats pre-treated with melatonin and/or TD. Methods: Wistar rats were divided in groups: 1) without AP; 2) AP without pre-treatment; 3) AP and Melatonin; 4) AP and TD; 5) AP and Melatonin associated to TD. Pancreatic tissue samples were collected for detection of SERCA2 and NCX1 m-R NA levels by polymerase chain reaction (PCR). Results: Increased m-RNA expression of SERCA2 in the melatonin treated group, without increase of m-RNA expression of the NCX1. The TD did not affect levels of SERCA2 and NCX1 m-RNA expressions. The combined melatonin and TD treatment reduced the m-RNA expression of SERCA2. Conclusions: The effect of melatonin is restricted to increased m-RNA expression of SERCA2. Although TD does not affect gene expression, its action in accelerating calcium exchanger function can explain the slightest expression of SERCA2 m-RNA when associated with Melatonin, perhaps by a joint action of drugs with different and but possibly complementary mechanisms.
  • conferenceObject
    Heparin Fragments Effects in Liver Injury Secondary to Liver Ischemia/Reperfusion (I/R)
    (2014) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; ABDO, Emilio E.; SAMPIETRE, Sandra N.; NADER, Helena B.; TERSARIOL, Ivarne S.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.
  • conferenceObject
    Trisulfated Disaccharide Decreases Intracellular Calcium Concentration in Hepatocytes Under Calcium Overload Situation
    (2015) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; CHAIB, Eleazar; NADER, Helena B.; TERSARIOL, Ivarne S.; ALBUQUERQUE, Luiz C. D.; RODRIGUES, Tiago
  • conferenceObject
    NEW FRAGMENT OF BOVINE HEPARIN FBH001 DECREASES INTRACELLULAR CALCIUM IN HEPATOCYTES SUBMITTED TO CALCIUM OVERLOAD
    (2019) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; NADER, Helena; TERSARIOL, Ivarne S.; LIMA, Marcelo A.; D'ALBUQUERQUE, Luiz Augusto C.; CHAIB, Eleazar; RODRIGUES, Tiago
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    THE MRNA EXPRESSION OF SERCA AND NCX IN ACUTE PANCREATITIS MODEL UNDER MELATONIN AND TRISULFATE DISSACHARIDE ACTION
    (2017) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; KUBRUSLY, Marcia S.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; RODRIGUES, Tiago; NADER, Helena; TERSARIOL, Ivarne S.; LIMA, Marcelo A.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.
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    THE MOLECULAR WEIGHT OF HEPARIN FRAGMENTS INTERFERES WITH THE PROTECTION OF THE HEPATOCYTE SUBJECTED TO INJURY BY ISCHEMIA AND REPERFUSION
    (2018) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; CHAIB, Eleazar; LIMA, Marcelo A.; NADER, Helena; TERSARIOL, Ivarne S.; D'ALBUQUERQUE, Luiz Augusto C.
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    Low Molecular Weight Heparin Fragment Decreases Intracellular Calcium in Human Hepatocarcinoma Cells Under Calcium Overload
    (2016) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; ABDO, Emilio E.; NADER, Helena B.; TERSARIOL, Ivarne S.; LIMA, Marcelo A.; D'ALBUQUERQUE, Luiz Augusto C.; CHAIB, Eleazar; RODRIGUES, Tiago
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    ACTION OF HEPARIN FRAGMENT ON CALCIUM DYNAMICS AND CELL MORTALITY IN A PANCREATIC CELL CULTURE UNDER TAUROCHOLATE EXPOSITION
    (2017) VASQUES, Enio R.; CUNHA, Jose Eduardo M.; COELHO, Ana Maria M.; SAMPIETRE, Sandra N.; KUBRUSLY, Marcia S.; CHAIB, Eleazar; D'ALBUQUERQUE, Luiz Augusto C.; NADER, Helena; LIMA, Marcelo A.; TERSARIOL, Ivarne S.; RODRIGUES, Tiago