ANA CRISTINA BREITHAUPT FALOPPA
Projetos de Pesquisa
Unidades Organizacionais
LIM/11 - Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação, Hospital das Clínicas, Faculdade de Medicina
6 resultados
Resultados de Busca
Agora exibindo 1 - 6 de 6
- Bilateral sympathectomy improves postinfarction left ventricular remodeling and function(2017) ZANONI, Fernando Luiz; SIMAS, Rafael; SILVA, Raphael Grillo da; BREITHAUPT-FALOPPA, Ana Cristina; SILVA, Raphael dos Santos Coutinho e; JATENE, Fabio Biscegli; MOREIRA, Luiz Felipe P.Objectives: To evaluate the influence of bilateral or left sympathectomy on left ventricular remodeling and function after myocardial infarction in rats. Methods: Myocardial infarction was induced in rats by ligation of the left anterior descending coronary. Seven days later, rats were divided into 4 groups: the myocardial infarction, myocardial infarction with left sympathectomy, myocardial infarction with bilateral sympathectomy, and sham groups. After 8 weeks, left ventricular function was evaluated with the use of a pressure-volume conductance catheter under steady-state conditions and pharmacological stress. Infarct size and extracellular matrix fibrosis were evaluated, and cardiac matrix metalloproteinases and myocardial inflammatory markers were analyzed. Results: The myocardial infarction and left sympathectomy group had an increased end diastolic volume, whereas the bilateral sympathectomy group had a mean end-diastolic volume similar to that of the sham group (P <. 002). Significant reduction in ejection fraction was observed in the myocardial infarction and left sympathectomy group, whereas it was preserved after bilateral sympathectomy (P < .001). In response to dobutamine, left ventricular contractility increased in sham rats, rising stroke work, cardiac output, systolic volume, end-diastolic volume, ejection fraction, and dP/dt max. Only bilateral sympathectomy rats had significant increases in ejection fraction (P <. 001) with dobutamine. Fibrotic tissue and matrix metalloproteinase expression decreased in the bilateral sympathectomy group compared to that in the myocardial infarction group (P <. 001) and was associated with left ventricular wall thickness maintenance and better apoptotic markers in noninfarcted myocardium. Conclusions: Bilateral sympathectomy effectively attenuated left ventricular remodeling and preserved systolic function after myocardial infarction induction in rats.
conferenceObject EFFECTS OF 17BETA-ESTRADIOL ON A SUDDEN ONSET BRAIN DEATH MODEL IN MALE RATS(2017) VIEIRA, Roberta Figueiredo; BREITHAUPT-FALOPPA, Ana Cristina; MATSUBARA, Bruno Carvalho; RODRIGUES, Geovana; SANCHES, Marcelo Petrof; FERREIRA, Sueli Gomes; CORREIA, Cristiano De Jesus; MOREIRA, Luiz Felipe; SANNOMIYA, Paulina- VAChT reduction increased mortality probably due to alveolar edema in a model of lung injury induced by intestinal isquemia/reperfusion in female mice(2017) SANTANA, Fernanda Paula Roncon; FANTOZZI, Evelyn; SILVA, Fernanda Yamamoto Ricardo da; PINHEIRO, Nathalia Montouro; TIBERIO, Iolanda de Fatima Lopes Calvo; MARTINS, Milton de Arruda; PRADO, Vania Ferreira; PRADO, Marco Antonio Maximo; LIMA, Wothan Tavares de; FALOPPA, Ana Cristina Breithaupt; PRADO, Carla Maximo
conferenceObject EFFECTS OF HYPERTONIC SALINE SOLUTION ON CARDIAC AND PULMONARY CHANGES AFTER BRAIN DEATH: AN EXPERIMENTAL STUDY(2017) CORREIA, Cristiano; MAGGALHAES, Daniel M.; ZANONI, Fernando; SIMAS, Rafael; SOARES, Rafaela Garcia Ferreira; SILVA, Raphael S. Coutinho E; BREITHAUPT-FALOPPA, Ana Cristina; SANNOMIYA, Paulina; MOREIRA, Luiz Felipe- ESTRADIOL MODULATES LOCAL GUT INJURY INDUCED BY INTESTINAL ISCHEMIA-REPERFUSION IN MALE RATS(2017) RICARDO-DA-SILVA, Fernanda Yamamoto; FANTOZZI, Evelyn Thais; RODRIGUES-GARBIN, Sara; OLIVEIRA-FILHO, Ricardo Martins; VARGAFTIG, Bernardo Boris; BREITHAUPT-FALOPPA, Ana Cristina; LIMA, Wothan Tavares deIntestinal ischemia and reperfusion (I/R) triggers a systemic inflammatory response characterized by leukocyte mobilization from the bone marrow, release of cytokines to the circulation, and increased microvascular permeability, leading to high mortality. Females have shown attenuated inflammatory response to trauma when compared with males, indicatinga role for female sex hormones in this process. Here, we have evaluated the effect of estradiol on the local gut injury induced by I/R in male rats. I/R was induced by the clamping of the superior mesenteric artery for 45 min, followed by 2 h of reperfusion. Agroup received 17 beta-estradiol (280 mu g/kg, i.v., single dose) at 30 min of ischemia. Morphometric analysis of the gut showed I/R induced a reduction of villous height that was prevented by estradiol. White blood cells, notably granulocytes, were mobilized from the circulation to the intestine by I/R, which was also prevented by estradiol treatment. Groups had the intestine wrapped in a plastic bag to collect intestinal fluid, where leukocytescount, TNF-alpha, and IL-10 levels were increased by I/R. Serum chemokines (CINC-1, MIP-1 alpha, MIP-2), ICAM-1 expression in the mesenteric tissue, and neutrophils spontaneous migration measured in vitro were also increased after I/R. Estradiol treatment reduced leukocytes numbers and TNF-alpha on intestinal fluid, serum chemokine release and also downregulated MIP-1 alpha, MIP-2 gene expression, and spontaneous in vitro neutrophil migration. In conclusion, estradiol blunts intestinal injury induced by I/R by modulating chemokines release and leukocyte trafficking.
conferenceObject ESTRADIOL TREATMENT REDUCES LUNG INFLAMMATION INDUCED BY BRAIN DEATH IN FEMALE RATS(2017) SILVA, Fernanda Yamamoto Ricardo Da; AMSTRONG JR., Roberto; SANTOS, Marina Vidal Dos; FERREIRA, Sueli Gomes; SANNOMIYA, Paulina; MOREIRA, Luiz Felipe; BREITHAUPT-FALOPPA, Ana Cristina