ANA CRISTINA BREITHAUPT FALOPPA
Projetos de Pesquisa
Unidades Organizacionais
LIM/11 - Laboratório de Cirurgia Cardiovascular e Fisiopatologia da Circulação, Hospital das Clínicas, Faculdade de Medicina
41 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 41
conferenceObject FEMALE RATS PRESENT HIGHER LUNG INFLAMMATION AFTER BRAIN DEATH FOLLOWED BY EX VIVO PERFUSION(2021) RICARDO-DA-SILVA, Fernanda Yamamoto; ARMSTRONG- JR., Roberto; OTTENS, Petra; ZANDEN, Judith van; VIDAL-DOS-SANTOS, Marina; MOREIRA, Luiz Felipe Pinho; ERASMUS, Michiel; LEUVENINK, Henri; BREITHAUPT-FALOPPA, Ana Cristina- Equity, Diversity and Inclusion (EDI) in Organ Transplantation: An ESOT Survey About EDI Within ESOT as an Organization and its Educational Activities, and Transplantation Research and Science(2023) PENGEL, L. H. M.; KAISAR, M.; BENJAMENS, S.; IBRAHIM, M.; RICCI, V.; BELLINI, M. I.; BREITHAUPT-FALOPPA, A. C.; FALK, C.; MAPLE, H.; MARSON, L.; ORTIZ, F.; PAPALOIS, V.; PAREDES, D.; FORSBERG, A.The European Society of Organ Transplantation (ESOT) strives to promote equity, diversity, and inclusion (EDI) across all its activities. We surveyed the transplant community's experiences and perspectives regarding EDI within ESOT as an organization and its educational activities, and research in general. A total of 299 respondents completed the questionnaire. About half agreed that ESOT's Executive Committee, Council, and Sections/Committees are diverse and inclusive (51%) and that ESOT promotes EDI in its live and digital educational activities (54%). Forty percent of respondents agreed that scientific and clinical trials in the field of transplantation are diverse and inclusive. Despite the wide distribution of the survey, most of the respondents self-identified as White and were either physician or surgeon. However, the results contribute a unique insight into the experiences and perspectives of the transplantation community regarding EDI. Whilst ESOT is committed to the principles of EDI, perceptions and the high number of proposals show the apparent need to prioritize efforts to embed EDI across ESOT and transplantation science. These data should constitute a starting point for change and provide guidance for future efforts to promote EDI within the transplantation community.
conferenceObject SEX-DIFFERENCES ON PLATELET AGGREGATION AND MICROVASCULAR PERFUSION AFTER BRAIN DEATH IN RATS(2019) CORREIA, Cristiano de Jesus; BREITHAUPT-FALOPPA, Ana Cristina; SILVA, Raphael dos Santos Coutinho e; SANTOS, Marina Vidal dos; ANUNCIACAO, Lucas Ferreira da; LEUVENINK, Hendrik Gerrit Derk; MOREIRA, Luiz Felipe Pinho- Estradiol mediates the long-lasting lung inflammation induced by intestinal ischemia and reperfusion(2018) FANTOZZI, Evelyn Thais; BREITHAUPT-FALOPPA, Ana Cristina; RICARDO-DA-SILVA, Fernanda Yamamoto; RODRIGUES-GARBIN, Sara; ROMERO, Daniel Cancelli; RODRIGUES, Adriana da Silva; RIFFO-VASQUEZ, Yanira; TAVARES-DE-LIMA, WothanBackground: Lung inflammation is one of the main consequences of intestinal ischemia reperfusion (intestinal IR) and, in severe cases, can lead to acute respiratory distress syndrome and death. We have previously demonstrated that estradiol exerts a protective effect on lung edema and cytokine release caused by intestinal IR in male rats. Materials and methods: We investigated the role of estradiol on the generation of interleukin (IL)-1 beta, IL-10, vascular endothelial growth factor (VEGF), and cytokine-induced neutrophil chemoattractant 1 (CINC-1) in a female rat model of intestinal IR. Blood and bone marrow leukocytes were also quantified. Seven-days-ovariectomized rats were subjected to intestinal IR by occlusion of the superior mesenteric artery for 45 min. After reperfusion of the tissue for 2 h, the rats were sacrificed. Lung tissue was collected, cultured for 24 h and assayed. Results: We observed a significant increase in serum levels of IL-10, CINC-1, uric acid and circulating, but not bone marrow, leukocyte numbers. In addition, intestinal IR induced a significant increase in the ex-vivo lung levels of IL-1 beta, IL-10, and VEGF. Treatment with 17b-estradiol before the induction of intestinal IR prevented the systemic release of IL-10, CINC-1, and uric acid, but it did not affect the leukocytosis. In addition, 17b-estradiol significantly prevented the ex-vivo release of IL-1b and VEGF from lung tissue. Conclusions: We demonstrated that intestinal IR interferes with lung homeostasis, priming the tissue to generate proinflammatory mediators for at least 24 h postischemia. Furthermore, our data confirm that the inflammatory responses caused by intestinal IR are estradiol mediated.
- Sex-related differences in lung inflammation after brain death(2016) BREITHAUPT-FALOPPA, Ana Cristina; FERREIRA, Sueli G.; KUDO, Guilherme K.; ARMSTRONG JR., Roberto; TAVARES-DE-LIMA, Wothan; SILVA, Luiz Fernando Ferraz da; SANNOMIYA, Paulina; MOREIRA, Luiz Felipe P.Background: Donor sex has been suggested to be a factor influencing organ transplantation outcome. Sex hormones possess inflammatory and immune-mediating properties; therefore, immune responses may differ between males and females. Brain death (BD) affects organ function by numerous mechanisms including alterations in hemodynamics, hormonal changes, and increased systemic inflammation. In this study, we investigated sex-dependent differences in the evolution of lung inflammation in a rat model of BD. Materials and methods: BD was induced by a sudden increase in intracranial pressure by rapidly inflating a balloon catheter inserted into the intracranial space. Groups of male, female, and ovariectomized (OVx) female rats were used. Lung vascular permeability, inducible nitric oxide synthase, and intercellular adhesion molecule 1 expression were analyzed 6 h after BD. Serum female sex hormones, vascular endothelial growth factor, and cytokine-induced neutrophil chemoattractant 1 levels were also quantified. Lung sections were analyzed by histology. Results: After 6 h of BD, serum estradiol and progesterone concentrations in female rats were significantly reduced. Lung microvascular permeability was increased in females compared to males. Cytokine-induced neutrophil chemoattractant 1 and vascular endothelial growth factor concentrations were increased in female rats compared to males. Furthermore, female rats showed higher levels of leukocyte infiltration and inducible nitric oxide synthase expression in the lung parenchyma. Conclusions: Our results indicate that the more severe lung inflammation in female animals after BD might be related to acute estradiol reduction. Based on our findings, we believe that, in a future study, a group of female treated with estradiol after BD could indicate a possible therapy for the control of lung inflammation in the female donor.
conferenceObject SEX DIMORPHISM IN INTESTINAL FUNCTION AFTER INTESTINAL ISCHEMIA/REPERFUSION INJURY(2015) FANTOZZI, Evelyn Thais; RICARDO-DA-SILVA, Fernanda Yamamoto; SANTIAGO-ROCHA, Barbara Lais; RODRIGUES-GARBIN, Sara; OLIVEIRA-FILHO, Ricardo Martins; BREITHAUPT-FALOPPA, Ana Cristina; TAVARES-DE-LIMA, Wothan- Sexual Dimorphism in Lung Inflammatory Process After Brain Death Induction in Rats(2014) MOREIRA, L. P.; FERREIRA, S. G.; KUDO, G. K.; CORREIRA, C. J.; TAVARES-DE-LIMA, W.; BREITHAUPT-FALOPPA, A. C.; SANNOMIYA, P.
- Bilateral sympathectomy improves postinfarction left ventricular remodeling and function(2017) ZANONI, Fernando Luiz; SIMAS, Rafael; SILVA, Raphael Grillo da; BREITHAUPT-FALOPPA, Ana Cristina; SILVA, Raphael dos Santos Coutinho e; JATENE, Fabio Biscegli; MOREIRA, Luiz Felipe P.Objectives: To evaluate the influence of bilateral or left sympathectomy on left ventricular remodeling and function after myocardial infarction in rats. Methods: Myocardial infarction was induced in rats by ligation of the left anterior descending coronary. Seven days later, rats were divided into 4 groups: the myocardial infarction, myocardial infarction with left sympathectomy, myocardial infarction with bilateral sympathectomy, and sham groups. After 8 weeks, left ventricular function was evaluated with the use of a pressure-volume conductance catheter under steady-state conditions and pharmacological stress. Infarct size and extracellular matrix fibrosis were evaluated, and cardiac matrix metalloproteinases and myocardial inflammatory markers were analyzed. Results: The myocardial infarction and left sympathectomy group had an increased end diastolic volume, whereas the bilateral sympathectomy group had a mean end-diastolic volume similar to that of the sham group (P <. 002). Significant reduction in ejection fraction was observed in the myocardial infarction and left sympathectomy group, whereas it was preserved after bilateral sympathectomy (P < .001). In response to dobutamine, left ventricular contractility increased in sham rats, rising stroke work, cardiac output, systolic volume, end-diastolic volume, ejection fraction, and dP/dt max. Only bilateral sympathectomy rats had significant increases in ejection fraction (P <. 001) with dobutamine. Fibrotic tissue and matrix metalloproteinase expression decreased in the bilateral sympathectomy group compared to that in the myocardial infarction group (P <. 001) and was associated with left ventricular wall thickness maintenance and better apoptotic markers in noninfarcted myocardium. Conclusions: Bilateral sympathectomy effectively attenuated left ventricular remodeling and preserved systolic function after myocardial infarction induction in rats.
conferenceObject ESTRADIOL TREATMENT MODULATES ESTRADIOL RECEPTORS EXPRESSION AND REDUCES RENAL INJURY AFTER BRAIN DEATH IN FEMALE RATS(2021) CORREIA, Cristiano; ARMSTRONG- JR., Roberto; RICARDO-DA-SILVA, Fernanda Yamamoto; VIDAL-DOS-SANTOS, Marina; ANUNCIACAO, Lucas Ferreira Da; MOREIRA, Luiz Felipe Pinho; LEUVENINK, Henri; BREITHAUPT-FALOPPA, Ana CristinaconferenceObject SEX DIFFERENCES ON DONOR LEUKOCYTE MOBILIZATION AND ORGAN INFLAMMATION AFTER BRAIN DEATH(2015) BREITHAUPT-FALOPPA, Ana Cristina; GOMES, Ferreira Sueli; KONISHI, Kudo Guilherme; RESTIVO, Simao Raif; ARMSTRONG JR., Roberto; SANNOMIYA, Paulina; MOREIRA, Luiz Felipe