FABIO LUIZ NAVARRO MARQUES
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Radiologia, Faculdade de Medicina
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
3 resultados
Resultados de Busca
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- Technetium glucose complexes as potential cancer imaging agents(2015) DAPUETO, Rosina; AGUIAR, Rodrigo B.; MORENO, Maria; MACHADO, Camila M. L.; MARQUES, Fabio L. N.; GAMBINI, Juan P.; CHAMMAS, Roger; CABRAL, Pablo; PORCAL, WilliamsGLUT's (facilitative glucose transporters) over-expression in tumor cells has allowed the detection of several cancer types, using a glucose analogue (F-18-FDG) with PET images, worldwide. New glucose analogs radiolabeled with Tc-99m could be a less-expensive and more accessible alternative for diagnosis using SPECT imaging. D-Glucose (Tc-99m-IDAG) and 2-D-deoxyglucose (Tc-99m-AADG) organometallic complexes were proposed and studied as potential F-18-FDG surrogates. The glucose complexes were prepared and evaluated as potential cancer imaging agents, in a melanoma tumor model. Iminodiacetic acid (IDA) and aminoacetate (AA) moieties were chosen as chelating system for radiolabeling with Tc-99m. Tumor uptake of the formed complexes was evaluated in B16 murine cell line in vitro and in vivo in melanoma bearing C57BL/6 mice. In vitro and in vivo studies were conducted with F-18-FDG in order to compare the uptake of Tc-99m-glucose complexes in the tumor model. IDAG and AADG compounds were synthesized and radiolabeled with (TcO4)-Tc-99m to obtain the Tc-99m-IDAG and Tc-99m-AADG complexes in high yield and stability. In vitro cell studies showed maximum uptake at 60 min for complexes, Tc-99m-IDAG and Tc-99m-AADG, with 6% and 2%, respectively. Biodistribution studies showed high tumor uptake one hour post-injection, reaching tumor-to-muscle ratios of 12.1 +/- 3.73 and 2.88 +/- 1.40 for Tc-99m-IDAG and Tc-99m-AADG, respectively. SPECT and micro-SPECT-CT images acquired after the injection of Tc-99m-IDAG showed accumulation in tumor sites, suggesting that this glucose complex would be a promising candidate for cancer imaging.
conferenceObject Preparation of new [[Tc-99m](MeOBzEN)(H2O)(CO)(3)](+) complexes and in vitro evaluation as potential tumor diagnostic radiopharmaceutical(2013) RADIN, Adriano; MARQUES, Fabio Luiz Navarro; NAGAI, Maria Aparecida; BUCHPIGUEL, Carlos AlbertoObjectives: In the present study we sought to prepare and evaluate the uptake of [[99m Tc](MeOBzEN)(H2O)(CO)3] + in breast cancer cells, MDA-MB-231, in suspension or in monolayer and to compare the results with [[99m Tc](MIBI)6] +, and a well-established radiopharmaceutical for breast cancer detection. Methods: The ligand L1: N-(4-methoxybenzil)ethylenediamine and L2: N,N ́-(di-4-methoxybenzil)ethylenediamine was synthesized by reaction between ethylenediamine and 4-methoxybenzaldehyde, followed by NaBH4 reduction. [[99m Tc](L1)(H2O)(CO)3] + and [[99m Tc](L2)(H2O)(CO)3] + (figure 1) were obtained by CO/NaBH4 standard reaction procedure, and quality controls (LogP, electrophoresis, HPLC) were performed. MDA-MB-23 was growth in RPMI-1640 culture media with 10% fetal bovine serum and part was allowed in suspension and other part was adhered in 12-well plates. Technetium radiopharmaceuticals were added to cells in suspension or monolayer and incubated at 37°C. At 10, 30 and 60 minutes, culture medium and cells were separated by centrifugation and activity was measured in a well counter. Figure 1. Structure of [[99m Tc](MeOBzEN)(H2O)(CO)3] + complex and uptake behavior in MDA-MB-231 cell culture (sus = suspension; mon = monolayer) Results: Ligands were obtained in 68% yield and technetium complex with overall yield high of 90% and were stable at least for four hours, LogP values were 0.266 ± 0.075 and 0.718 ± 0.018, for complex L1 and L2, against 1.330 ± 0.127 for MIBI. Complexes charge was inconclusive, once it depends of electrolitic system. Uptakes of both new complexes were high as compared to [[99m Tc](MIBI)6] +, and the value depends if cells are in suspension or adhered, as show in figure 1. Conclusions: The new [[99mTc](MeOBzEN)(H2O)(CO)3]+ were obtained in high yields and uptake in MDA-MD-231 culture cell is higher than [[99m Tc](MIBI)6]+, suggesting a potential use as radiopharmaceutical. However, it's not clear difference between uptake in suspension and adhered cell and further investigation are been undertaken, including animal imaging.conferenceObject Preparation of Tc-99m-BDTC complexes and evaluation as tumor marker(2015) LAFRATTA, Alyne E.; FERNANDES, Andre G. Araujo; DEFLON, Victor M.; BUCHPIGUEL, Carlos A.; MARQUES, Fabio L. Navarro