MARIA NOTOMI SATO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Dermatologia, Faculdade de Medicina - Docente
LIM/56 - Laboratório de Investigação em Dermatologia e Imunodeficiências, Hospital das Clínicas, Faculdade de Medicina - Líder
LIM/56 - Laboratório de Investigação em Dermatologia e Imunodeficiências, Hospital das Clínicas, Faculdade de Medicina - Líder
8 resultados
Resultados de Busca
Agora exibindo 1 - 8 de 8
- Infrared low-level diode laser on serum chemokine MCP-1 modulation in mice(2013) FUKUDA, Thiago Y.; TANJI, Maury M.; JESUS, Julio Fernandes de; SILVA, Suelen Rocha da; SATO, Maria N.; PLAPLER, HelioThe effect of the low-level laser therapy (LLLT) in the modulation of cells related to inflammatory processes has been widely studied, with different parameters. The objective was to investigate the immediate and cumulative effect of infrared LLLT on chemokine monocyte chemotactic protein-1 (MCP-1) modulation in mice. Fifty-two isogenic mice were distributed in seven groups: control (n = 10, no surgical procedure), laser I (n = 7, surgical procedure and a single LLLT exposure 12 h after the surgery), laser II (n = 7, surgery followed by two LLLT exposures, 12 and 36 h after surgery), and laser III (n = 7, surgery followed by three LLLT exposures, 12, 36, and 60 h after surgery). For each group, a sham group (n = 21) underwent surgery without laser application. The animals in the laser groups received an infrared diode continuous laser exposure (AsGaAl, 780 nm wavelength, power of 20 mW, energy density of 10 J/cm(2), spot size of 0,04 cm(2)) on three points (20 s per point), and a final energy of 0.4 J. The animals were sacrificed 36 h (laser I and sham I groups), 60 h (laser II and sham II), and 84 h (laser III and sham III groups) after surgery. The MCP-1 concentrations were measured by cytometric bead array. There was no significant difference between the three periods in the sham group (p = 0.3). There was a lower concentration of MCP-1 in the laser III group compared to the laser I group (p = 0.05). The infrared LLLT showed a cumulative effect in the modulation of chemokine MCP-1 concentration. Three LLLT exposures were necessary to achieve the MCP-1 modulation.
conferenceObject Atopic dermatitis in adults: Augmented circulating IgG4 and IgE antibodies against Staphylococcus aureus enterotoxin B(2013) ORFALI, R. L.; SATO, M. N.; SANTOS, V. G. Dos; TITZ, T. O.; DUARTE, A. S.; TAKAOKA, R.; AOKI, V.The aim of this study was to evaluate the profile of anti-Staphylococcus aureus enterotoxin B (SEB) antibody (Ab) response in adults with AD. We selected 38 patients diagnosed as AD (Hanifin & Rajka's criteria), aged between 18 and 65, and 26 healthy controls (HC), aged between 20 and 47 years. Severity of the disease was established according to EASI (Eczema Area and Severity Index) and patients were graded as mild (28%), moderate (58%) and severe (14%). Sera were assessed for IgG subclasses, IgA, IgM and IgE against SEB by ELISA. The humoral response in AD patients to SEB showed elevated circulating IgE and IgG4 levels (p < 0.05 and p δ 0.001, respectively), and decreased IgA and IgM levels (p < 0.05). Severity of atopic dermatitis was related with low IgG1 and IgG3 levels (p < 0.05), and high IgE antibody response (p < 0.05) to SEB. Interestingly, absence of IgE and IgG1 response to SEB was seen in some AD patients (26.3% and 18.4% respectively). In AD, the immunoglobulin-related subtype Th2 lymphocytes, such as IgE and IgG4, appear to be relevant in the response to superantigens. These results emphasize an altered pattern of Ab response to SEB in AD, which is impaired according the disease severity.- Infrared low-level diode laser on inflammatory process modulation in mice: pro- and anti-inflammatory cytokines(2013) FUKUDA, Thiago Y.; TANJI, Maury M.; SILVA, Suelen R.; SATO, Maria N.; PLAPLER, HelioTo evaluate the modulation of proinflammatory (interleukin-6, IL-6; tumor necrosis factor-alpha, TNF-alpha; and interferon-gamma, IFN-gamma) and anti-inflammatory cytokines (transforming growth factor-beta 1, TGF-beta 1) in the inflammation processes in vivo with low-level laser action, 50 isogenic mice were randomly distributed into three groups: control (no surgical procedure, n = 10), sham (surgical procedure with three standard cutaneous incisions, followed by an abdominal muscle incision and suture, n = 20), and laser (same procedure followed by laser exposure, n = 20). The sham group was divided into three subgroups: sham I (euthanasia and evaluation, 36 h after surgical procedure), sham II (euthanasia and evaluation, 60 h after surgical procedure), and sham III (euthanasia and evaluation, 84 h after surgical procedure). The laser group was also divided in three subgroups: laser I (a single laser session, 12 h after surgery), laser II (two laser sessions, 12 and 36 h after surgery), and laser III (three laser sessions, 12, 36, and 60 h after surgery). All animals in the laser groups received three points per session of continuous infrared laser (wavelength of 780 nm, power of 20 mW, fluency of 10 J/cm(2), exposure time of 20 s per point, and energy of 0.4 J). After euthanasia, spleen mononuclear cells were isolated and cultured for 48 h. Concentrations of IL-6, TNF-alpha, IFN-gamma, and TGF-beta 1 were obtained by enzyme-linked immunosorbent assay method. There was a significant difference between the IL-6 and TNF-alpha concentrations in the 60-and 84-h evaluations when the laser and sham groups were compared to the control group (p < 0.05), except for laser II in the TNF-alpha analysis (p > 0.05). The IFN-gamma concentration analysis showed a significant difference only in sham II when compared to the control group (p < 0.05). Thus, there was a modulatory effect of TNF-alpha and IFN-gamma in the laser group, particularly in the 60-h postoperative evaluation. There was no significant difference between the laser, sham, and control groups for TGF-beta 1 analysis (p > 0.05). The low-level laser application decreased the TNF-alpha and IFN-gamma release in vivo of spleen mononuclear cells in mice, especially after two exposure sessions. However, there was no modulation of the IL-6 and TGF-beta 1 release.
- Upregulation of Innate Antiviral Restricting Factor Expression in the Cord Blood and Decidual Tissue of HIV-Infected Mothers(2013) PEREIRA, Natalli Zanete; CARDOSO, Elaine Cristina; OLIVEIRA, Luanda Mara da Silva; LIMA, Josenilson Feitosa de; BRANCO, Anna Claudia Calvielli Castelo; RUOCCO, Rosa Maria de Souza Aveiro; ZUGAIB, Marcelo; OLIVEIRA FILHO, Joao Bosco de; DUARTE, Alberto Jose da Silva; SATO, Maria NotomiPrograms for the prevention of mother-to-child transmission of HIV have reduced the transmission rate of perinatal HIV infection and have thereby increased the number of HIV-exposed uninfected (HEU) infants. Natural immunity to HIV-1 infection in both mothers and newborns needs to be further explored. In this study, we compared the expression of antiviral restricting factors in HIV-infected pregnant mothers treated with antiretroviral therapy (ART) in pregnancy (n=23) and in cord blood (CB) (n=16), placental tissues (n=10-13) and colostrum (n=5-6) samples and compared them to expression in samples from uninfected (UN) pregnant mothers (n=21). Mononuclear cells (MNCs) were prepared from maternal and CB samples following deliveries by cesarean section. Maternal (decidua) and fetal (chorionic villus) placental tissues were obtained, and colostrum was collected 24 h after delivery. The mRNA and protein expression levels of antiviral factors were then evaluated. We observed a significant increase in the mRNA expression levels of antiviral factors in MNCs from HIV-infected mothers and CB, including the apolipoprotein B mRNA-editing enzyme 3G (A3G), A3F, tripartite motif family-5 alpha (TRIM-5 alpha), TRIM-22, myxovirus resistance protein A (MxA), stimulator of interferon (IFN) genes (STING) and IFN-beta, compared with the levels detected in uninfected (UN) mother-CB pairs. Moreover, A3G transcript and protein levels and alpha-defensin transcript levels were decreased in the decidua of HIV-infected mothers. Decreased TRIM-5 alpha protein levels in the villi and increased STING mRNA expression in both placental tissues were also observed in HIV-infected mothers compared with uninfected (UN) mothers. Additionally, colostrum cells from infected mothers showed increased tetherin and IFN-beta mRNA levels and CXCL9 protein levels. The data presented here indicate that antiviral restricting factor expression can be induced in utero in HIV-infected mothers. Future studies are warranted to determine whether this upregulation of antiviral factors during the perinatal period has a protective effect against HIV-1 infection.
- TLR7/TLR8 Activation Restores Defective Cytokine Secretion by Myeloid Dendritic Cells but Not by Plasmacytoid Dendritic Cells in HIV-Infected Pregnant Women and Newborns(2013) CARDOSO, Elaine Cristina; PEREIRA, Natalli Zanete; MITSUNARI, Gabrielle Eimi; OLIVEIRA, Luanda Mara da Silva; RUOCCO, Rosa Maria S. A.; FRANCISCO, Rossana Pulcineli Vieira; ZUGAIB, Marcelo; DUARTE, Alberto Jose da Silva; SATO, Maria NotomiMother-to-child transmission (MTCT) of HIV-1 has been significantly reduced with the use of antiretroviral therapies, resulting in an increased number of HIV-exposed uninfected infants. The consequences of HIV infection on the innate immune system of both mother-newborn are not well understood. In this study, we analyzed peripheral blood and umbilical cord blood (CB) collected from HIV-1-infected and uninfected pregnant women. We measured TNF-alpha, IL-10 and IFN-alpha secretion after the stimulation of the cells with agonists of both extracellular Toll-like receptors (TLRs) (TLR2, TLR4 and TLR5) and intracellular TLRs (TLR7, TLR7/8 and TLR9). Moreover, as an indicator of the innate immune response, we evaluated the responsiveness of myeloid dendritic cells (mDCs) and plasmacytoid DCs (pDCs) to TLRs that are associated with the antiviral response. Our results showed that peripheral blood mononuclear cells (PBMCs) from HIV-1-infected mothers and CB were defective in TNF-alpha production after activation by TLR2, TLR5, TLR3 and TLR7. However, the TNF-alpha response was preserved after TLR7/8 (CL097) stimulation, mainly in the neonatal cells. Furthermore, only CL097 activation was able to induce IL-10 and IFN-alpha secretion in both maternal and CB cells in the infected group. An increase in IFN-alpha secretion was observed in CL097-treated CB from HIV-infected mothers compared with control mothers. The effectiveness of CL097 stimulation was confirmed by observation of similar mRNA levels of interferon regulatory factor-7 (IRF-7), IFN-alpha and TNF-alpha in PBMCs of both groups. The function of both mDCs and pDCs was markedly compromised in the HIV-infected group, and although TLR7/TLR8 activation overcame the impairment in TNF-alpha secretion by mDCs, such stimulation was unable to reverse the dysfunctional type I IFN response by pDCs in the HIV-infected samples. Our findings highlight the dysfunction of innate immunity in HIV-infected mother-newborn pairs. The activation of the TLR7/8 pathway could function as an adjuvant to improve maternal-neonatal innate immunity.
- Atopic dermatitis in adults: clinical and epidemiological considerations(2013) ORFALI, Raquel Leao; SHIMIZUA, Marta M.; TAKAOKA, Roberto; ZANIBONI, Mariana C.; ISHIZAKI, Aline S.; COSTA, Anderson A.; TIBA, Ana Paula L.; SATO, Maria Notomi; AOKI, ValeriaObjective: Atopic dermatitis (AD) is a chronic inflammatory disease causing intense pruritus, and with typical clinical features. There are few epidemiological studies concerning AD in adults, aswell as little information about its prognostic. The aim of this study was to evaluate the clinical and epidemiological course of adults with AD. Methods: 80 patients aged above 18 years (mean age = 29 years) were selected (30 males and 50 females) and interviewed about hospitalization, systemic corticoid usage, age of ADonset, and personal and/or familial history of atopy. Disease severity was evaluated through the Scoring Atopic Dermatitis (SCORAD) tool. Laboratory examination included IgE serum levels and eosinophil blood count. Results: 71 out of 80 patients referred association with respiratory symptoms (18 had asthma, 17 had rhinitis, and 36 had both conditions); nine out of 80 patients denied any respiratory disease. AD patients were divided in mild (n = 25), moderate (n = 30), and severe (n = 25); 56% had one or more hospitalizations due to AD. A positive association was found between IgE serum levels, eosinophil blood count, and disease severity. Conclusion: Adult AD represents a clinical challenge that needs to be better characterized, since it can be misdiagnosed and interferes with the patient's social and personal life. The association of skin and respiratory atopic disease is frequent, and laboratory parameters such as circulating IgE levels and eosinophil blood count may be helpful to assess disease severity.
conferenceObject Impaired APOBEC3G and TRIM-5 alpha expression in decidual and villi tissue of mothers infected by HIV-1(2013) PEREIRA, N. Z.; CARDOSO, E. C.; MITSUNARI, G. E.; RUOCCO, R. M. S. A.; ZUGAIB, M.; OLIVEIRA-FILHO, J. B.; DUARTE, A. J. S.; SATO, M. N.Background: A high percentage of infants from mothers infected by HIV-1 was uninfected even in the absence of antiretroviral therapy. The purpose of this study was to evaluate the expression of antiviral factors in HIV-infected mothers and cord blood (CB), compared with uninfected mothers-CB. Methods: The antiviral factors were evaluated in PBMC, decidual and placental villi tissue. The study was approved by the ethics committee of no 0034/11. Results: High mRNA of antiviral factors was detectable in PBMC of HIV-infected mothers-CB. Furthermore, despite of normal transcriptional APOBEC3G and TRIM-5α, the protein expression was significantly decreased in decidual and villi tissue, respectively, in HIV-infected mothers compared to control mothers. Conclusion: HIV infection may induce an impairment of antiviral factors at post-transcriptional levels at the maternal-fetal interface, with an enhancement of these factors at peripheral blood.conferenceObject Maternal allergen immunization in mice: Prevention of offspring early allergen sensitization(2013) VICTOR, J. R.; OLIVEIRA, L. M.; LIRA, A. A. L.; MUNIZ, B. P.; DUARTE, A. J. S.; SATO, M. N.Background: Previously we have been observed that pre-conceptional maternal immunization to OVA is able to prevent the development of allergy in mice. Methods: Female mice were immunized with OVA and mated with normal males. Offspring were evaluated at 3 days old (d.o.) or immunized with OVA and evaluated at 20 d.o. Results: Maternal immunization up-regulated the expression of FcγRIIb on offspring B cells at 3 and 20 d.o. Serum analysis revealed a down regulation on offspring IL-6 levels. Newborns show similar levels of TCD4+ cells producing IL-10, IL-17, IFN-γ or IL-4. Offspring from immune-mothers shows augmented IL-10+ and IL-17+ B cell levels. After offspring immunization we observed higher levels of IL-17+ and IFN-γ+ TCD4+ cells and IL-17+ and IFN-γ+ B cells of offspring from immune mothers. Conclusion: We confirmed the up-regulation of FcγRIIb on offspring B cells, and the augmented IL-10+ B cells on offspring suggest Breg cells involvement. The role of IL-17 must be investigated.