MARA DE SOUZA JUNQUEIRA
Projetos de Pesquisa
Unidades Organizacionais
Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas, Faculdade de Medicina
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
LIM/43 - Laboratório de Medicina Nuclear, Hospital das Clínicas, Faculdade de Medicina
LIM/24 - Laboratório de Oncologia Experimental, Hospital das Clínicas, Faculdade de Medicina
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- Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?(2022) FERNANDES, Bianca H. Ventura; FEITOSA, Natalia Martins; BARBOSA, Ana Paula; BOMFIM, Camila Gasque; GARNIQUE, Anali M. B.; ROSA, Ivana F.; RODRIGUES, Maira S.; DORETTO, Lucas B.; COSTA, Daniel F.; CAMARGO-DOS-SANTOS, Bruno; FRANCO, Gabrielli A.; FAVERO NETO, Joao; LUNARDI, Juliana Sartori; BELLOT, Marina Sanson; ALVES, Nina Pacheco Capelini; COSTA, Camila C.; ARACATI, Mayumi F.; RODRIGUES, Leticia F.; CIRILO, Rafaela Hemily; COLAGRANDE, Raul Marcelino; GOMES, Francisco I. F.; NAKAJIMA, Rafael T.; BELO, Marco A. A.; GIAQUINTO, Percilia Cardoso; OLIVEIRA, Susana Luporini de; ETO, Silas Fernandes; FERNANDES, Dayanne Carla; MANRIQUE, Wilson G.; CONDE, Gabriel; ROSALES, Roberta R. C.; TODESCHINI, Iris; RIVERO, Ilo; LLONTOP, Edgar; SGRO, German G.; OKA, Gabriel Umaji; BUENO, Natalia Fernanda; FERRARIS, Fausto K.; MAGALHAES, Mariana T. Q. de; MEDEIROS, Renata J.; MENDONCA-GOMES, Juliana M.; JUNQUEIRA, Mara Souza; CONCEICAO, Katia; PONTES, Leticia Gomes de; CONDINO-NETO, Antonio; PEREZ, Andrea C.; BARCELLOS, Leonardo J. G.; CORREA JUNIOR, Jose Dias; DORLASS, Erick Gustavo; CAMARA, Niels O. S.; DURIGON, Edison Luiz; CUNHA, Fernando Q.; NOBREGA, Rafael H.; MACHADO-SANTELLI, Glaucia M.; FARAH, Chuck S.; VERAS, Flavio P.; GALINDO-VILLEGAS, Jorge; V, Leticia Costa-Lotufo; CUNHA, Thiago M.; CHAMMAS, Roger; CARVALHO, Luciani R.; GUZZO, Cristiane R.; MALAFAIA, Guilherme; CHARLIE-SILVA, IvesDespite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.