JEANNE DA ROSA OITICICA RAMALHO
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina
LIM/32 - Laboratório de Otorrinolaringologia, Hospital das Clínicas, Faculdade de Medicina
LIM/32 - Laboratório de Otorrinolaringologia, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
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- Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate in vivo and Promote Facial Nerve Regeneration(2019) PEREIRA, Larissa Vilela; BENTO, Ricardo Ferreira; CRUZ, Dayane B.; MARCHI, Claudia; SALOMONE, Raquel; OITICICCA, Jeanne; COSTA, Marcio Paulino; HADDAD, Luciana A.; MINGRONI-NETTO, Regina Celia; COSTA, Heloisa Juliana Zabeu RossiPost-traumatic lesions with transection of the facial nerve present limited functional outcome even after repair by gold-standard microsurgical techniques. Stem cell engraftment combined with surgical repair has been reported as a beneficial alternative. However, the best association between the source of stem cell and the nature of conduit, as well as the long-term postoperative cell viability are still matters of debate. We aimed to assess the functional and morphological effects of stem cells from human exfoliated deciduous teeth (SHED) in polyglycolic acid tube (PGAt) combined with autografting of rat facial nerve on repair after neurotmesis. The mandibular branch of rat facial nerve submitted to neurotmesis was repaired by autograft and PGAt filled with purified basement membrane matrix with or without SHED. Outcome variables were compound muscle action potential (CMAP) and axon morphometric. Animals from the SHED group had mean CMAP amplitudes and mean axonal diameters significantly higher than the control group (p < 0.001). Mean axonal densities were significantly higher in the control group (p = 0.004). The engrafted nerve segment resected 6 weeks after surgery presented cells of human origin that were positive for the Schwann cell marker (S100), indicating viability of transplanted SHED and a Schwann cell-like phenotype. We conclude that regeneration of the mandibular branch of the rat facial nerve was improved by SHED within PGAt. The stem cells integrated and remained viable in the neural tissue for 6 weeks since transplantation, and positive labeling for S100 Schwann-cell marker suggests cells initiated in vivo differentiation.
- Video head impulse test relevance in the early postoperative period after cochlear implantation(2019) BITTAR, Roseli Saraiva Moreira; SATO, Eduardo; RIBEIRO, Douglas Josimo Silva; OITICICA, Jeanne; GRASEL, Signe Schuster; MEZZALIRA, Raquel; TSUJI, Robinson Koji; BENTO, Ricardo FerreiraBackground: Cochlear implantation (CI) is the gold standard therapy for profound or severe sensorineural hearing loss. It is a safe surgical procedure but, because of the proximity of the cochlea and vestibule, postoperative vestibular disorder may occur. Our hypothesis is that the video head impulse test (vHIT) may be a good tool to achieve a topographic diagnosis of dizziness in the early postoperative period after CI. Aims/Objectives: To evaluate patients with instability, imbalance and vertigo between 7 and 14 days after CI procedure. Material and methods: A total of 31patients scheduled for unilateral CI were included in this study. vHIT for horizontal semicircular canal was performed before CI and between days 7 to 14 after the surgery. Results: Six subjects had dizziness complaints after CI: instability (N = 2), imbalance (N = 2) and vertigo (N = 2). The postoperative vHIT test turned abnormal only in subjects with vertigo as compared to the preoperative vHIT test results. Conclusion and significance: vHIT is a good vestibular function test during the first 2 weeks after CI surgery when vertigo is the main complaint
- Caloric test and video head impulse test sensitivity as vestibular impairment predictors before cochlear implant surgery(2019) BITTAR, Roseli Saraiva Moreira; SATO, Eduardo Setsuo; SILVA-RIBEIRO, Douglas Josimo; OITICICA, Jeanne; MEZZALIRA, Raquel; TSUJI, Robinson Koji; BENTO, Ricardo FerreiraOBJECTIVES: Currently, cochlear implant procedures are becoming increasingly broad and have greatly expanded. Bilateral cochlear implants and cochlear implants are more frequently applied in children. Our hypothesis is that the video head impulse test may be more sensitive than the caloric test in detecting abnormal vestibular function before cochlear implant surgery. The objective of this study was to compare the video head impulse test and caloric test results of patients selected for cochlear implant procedures before surgery. METHODS: The patients selected for cochlear implant surgery were submitted to a bithermal caloric test and video head impulse test. RESULTS: By comparing angular slow phase velocity values below 5 degrees in the bithermal caloric test (hypofunction) and video head impulse test with a gain lower than 0.8, we identified 37 (64.9%) patients with vestibular hypofunction or canal paresis and 21 (36.8%) patients with abnormal video head impulse test gain before the cochlear implant procedure. Of the 37 patients with caloric test vestibular hypofunction, 20 (54%) patients exhibited an abnormal gain in the video head impulse test. CONCLUSION: The caloric test is more sensitive than the video head impulse test (Fisher's exact test, p=0.0002) in detecting the impaired ear before cochlear implant delivery. The proportion of caloric test/video head impulse test positive identification of abnormal vestibular function or caloric test/video head impulse test sensitivity was 1.8:1.
- Stem-cell therapy for hearing loss: are we there yet?(2019) DUFNER-ALMEIDA, Luiz Gustavo; CRUZ, Dayane Bernardino da; MINGRONI NETTO, Regina Celia; BATISSOCO, Ana Carla; OITICICA, Jeanne; SALAZAR-SILVA, RodrigoIntroduction: Mammalian hair cells and auditory neurons do not show regenerative capacity. Hence, damage to these cell types is permanent and leads to hearing loss. However, there is no treatment that re-establishes auditory function. Regenerative therapies using stem cells represent a promising alternative. Objective: This article aims to review the current literature about the main types of stem cells with potential for application in cell therapy for sensorineural hearing loss, the most relevant experiments already performed in animals, as well as the advances that have been recently made in the field. Methods: Research included the databases PubMed/MEDLINE, Web of Science, Science Direct and SciELO, as well as gray literature. Search strategy included the following main terms: ""stem cells'', ""hair cells'' and ""auditory neurons''. Additionally, the main terms were combined with the following secondary terms: ""mesenchymal'', ""iPS'', ""inner ear'', ""auditory''. The research was conducted independently by three researchers. Results: Differentiation of stem cells into hair cells and auditory neurons has a high success rate, reaching up to 82% for the first and 100% for the latter. Remarkably, these differentiated cells are able to interact with hair cells and auditory neurons of cochlear explants through formation of new synapses. When transplanted into the cochlea of animals with hearing loss, auditory restoration has been documented to date only in deafferented animals. Conclusion: Advances have been more prominent in cases of auditory neuropathy, since partial improvement of auditory nerve conditions through cell-based therapy may increase the number of patients who can successfully receive cochlear implants. (C) 2019 Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial.
- Recording of tinnitus psychoacoustic measurements: an integrative literature review(2019) GUIJO, Laura Mochiatti; FONSECA, Angela Renata Sarambelli; HORIUTI, Mirella Boaglio; VASCONCELOS, Laura Garcia E.; CARDOSO, Ana Claudia Vieira; OITICICA, JeanneABSTRACT Purpose: to review both the national and international literature as well as to describe the methods used to collect psychoacoustic measurements in tinnitus patients. Methods: the current integrative review was conducted on articles in specialized national and international journals, in both the Portuguese and English languages, available in the PubMed/Medline, BVS - LILACS and SCIELO databases. The following keywords and descriptors were used: hearing, tinnitus, psychoacoustic measurements, acuphenometry, and assessment, in both the Portuguese and English languages. The retrieved articles were read and selected for the review according to the eligibility criteria, which included the use of psychoacoustic measurements in tinnitus patients and the presence of a detailed methodological description of the protocol employed, in individuals with normal hearing and those with hearing loss. Results: a total of 12 articles, in which psychoacoustic measures were used for the characterization and the measurement of tinnitus in individuals with normal hearing and in those with hearing loss, were reviewed. The main findings were associated with the sample characterization and the way in which the psychoacoustic measures were performed. Conclusion:given the grouping of several protocols for tinnitus evaluation, which were determined following an integrative literature review, a great heterogeneity in the methods used to perform psychoacoustic measurements for tinnitus assessment for both clinical and scientific purposes was noted.