MARCELO LUIZ BALANCIN
Projetos de Pesquisa
Unidades Organizacionais
7 resultados
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- Comprehensive analysis of immune, extracellular matrices and pathogens profile in lung granulomatosis of unexplained etiology(2018) SOUZA, Paola da Costa; DONDO, Patricia Suemi; SOUZA, Gabriela; LOPES, Deborah; MOSCARDI, Marcel; MARTINHO, Vinicius de Miranda; LOURENCO, Rodolfo Daniel de Mattos; PRIETO, Tabatha; BALANCIN, Marcelo Luiz; ASSATO, Aline Kawassaki; TEODORO, Walcy Rosolia; RODRIGUES, Silvia; LIMA, Mariana; CASTELLANO, Maria Vera; COLETTA, Ester; PARRA, Edwin Roger; CAPELOZZI, Vera LuizaThis study analyzed the type 1 and type 2 T helper (Th1/Th2) cytokines (including interleukins), immune cellular, matrix profile, and pathogens in granulomas with unexplained etiology compared to those with infectious and noninfectious etiology. Surgical lung biopsies from 108 patients were retrospectively reviewed. Histochemistry, immunohistochemistry, immunofluorescence, morphometry and polymerase chain reaction were used, respectively, to evaluate total collagen and elastin fibers, collagen I and III, immune cells, cytokines, matrix metalloproteinase-9, myofibroblasts, and multiple usual and unusual pathogens. No relevant polymerase chain reaction expression was found in unexplained granulomas. A significant difference was found between the absolute number of eosinophils, macrophages, and lymphocytes within granulomas compared to uninvolved lung tissue. Granulomas with unexplained etiology (UEG) presented increased number of eosinophils and high expression of interleukins (ILs) IL-4/IL-5 and transforming growth factor-beta. In sarcoidosis, CD4/CD8 cell number was significantly higher within and outside granulomas, respectively; the opposite was detected in hypersensitivity pneumonitis. Again, a significant difference was found between the high number of myofibroblasts and matrix metalloproteinase-9 in UEG, hypersensitivity pneumonitis, and sarcoidosis compared to granulomas of tuberculosis. Granulomas of paracoccidioisis exhibited increased type I collagen and elastic fibers. Th1 immune cellular profile was similar among granulomas with unexplained, infectious, and noninfectious etiology. In contrast, modulation of Th2 and matrix remodeling was associated with more fibroelastogenesis and scarring of lung tissue in UEG compared to infectious and noninfectious. We concluded that IL-4/IL-5 and transforming growth factor-beta might be used as surrogate markers of early fibrosis, reducing the need for genotyping, and promise therapeutic target in unexplained granulomas.
conferenceObject Paratesticular fibrous pseudotumour and the importance of immunohistochemical evaluation of IgG4/IgG ratio(2018) MEDEIROS, G.; MORGANTETTI, G.; BALANCIN, M.; CAPELOZZI, V.- Histomorphometric evaluation of the Ki-67 proliferation rate and CD34 microvascular and D2-40 lymphovascular densities drives the pulmonary typical carcinoid outcome(2018) VILHENA, Alyne Fonseca de; PEREIRA, Joao Carlos das Neves; PARRA, Edwin Roger; BALANCIN, Marcelo Luiz; AB'SABER, Alexandre; MARTINS, Vanessa; FARHAT, Cecilia; ABRANTES, Marcelo Militao; CAMPOS, Jose Ribas Milanez de; TEDDE, Miguel Lia; TAKAGAKI, Teresa; CAPELOZZI, Vera LuizaKi-67 has shown promise as a prognostic factor in pulmonary carcinoids. In this study, we sought to validate the importance of Ki-67 and study the relationships between Ki-67 and other stromal biomarkers of vascular density. We examined Ki-67, CD34, and D2-40 in tumor tissues from 128 patients with surgically excised typical carcinoid of the lung. We used immunohistochemistry and morphometry to evaluate the amount of tumor staining for cellular proliferation (Ki-67), microvascular density (CD34-MVD), and D2-40 lymphovascular density. The main outcome was overall survival, considered as life expectancy until death from metastasis. Specimens from patients with central tumors showed high CD34-MVD (P = .01), which was also significantly associated with a compromised surgical margin, lymph node metastasis, and clinical stage Ib. Equally significant was high D2-40 lymphovascular density in central specimens with a compromised surgical margin and lymph node metastasis. A high Ki-67 proliferation rate was significantly associated with tumors from patients with clinical stage IIb, IIIa, and IV disease. Multivariate Cox model analysis demonstrated that tumor location and stage, surgical margin, tumor size, and N stage were significantly related to survival time (P < .05). Quantitative staining of the tumor for Ki-67 and CD34-MVD served as prognostic factors (P < .05), which were more relevant than the surgical and pathological stage. Ki-67 greater than 5% and CD34-MVD greater than 7% staining comprise a subset of patients with higher death hazard; this outcome may harbor evidence for further prospective studies of target therapy after surgical resection.
conferenceObject Mesothelioma grading system and pleural, extra-pleural and histologic subtypes differences in a Latin-American cohort of patients (preliminary data)(2018) MEDEIROS, G.; MORGANTETTI, G.; BALANCIN, M.; AB'SABER, A.; MARTINS, V.; CAPELOZZI, V.conferenceObject Causality establishment in surgical lung and forensics pathology through characterisation of inorganic tissue - a case report with the Maltese cross example(2018) MEDEIROS, G.; MORGANTETTI, G.; BALANCIN, M.; CAPELOZZI, V.; AB'SABER, A.conferenceObject Copper pennies in the tropics: chromoblastomycosis, a diagnosis not to be forgotten on tropical pathology(2018) MEDEIROS, G.; MORGANTETTI, G.; BALANCIN, M.; FABRO, A.; CAPELOZZI, V.- Association of Functional Polymorphism in CTLA-4 Gene with Survival in Non-Small Cell Lung Cancer: A Brazilian Study(2018) MACHADO-RUGOLO, J.; FABRO, A.; CUENTAS, E.; SA, V. De; RAINHO, C.; NAGAI, M.; CAPELOZZI, V.; BALANCIN, M.