MARCIA KIYOMI KOIKE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
11 resultados
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conferenceObject Palmitic Interesterified Fat Induces Atherosclerosis and Inflammatory Cytokine Secretion in LDL Receptor Knockout Mice(2014) AFONSO, Milessa S.; LAVRADOR, Maria Silvia F.; KOIKE, Marcia; BOMBO, Renata P.; NUNES, Valeria S.; CATANOZI, Sergio; CASTILHO, Gabriela; PASSARELLI, Marisa; NAKANDAKARE, Edna R.; LOTTENBERG, Ana Maria- Increased intestinal production of alpha-defensins in aged rats with acute pancreatic injury(2014) CUNHA, Debora Maria Gomes; KOIKE, Marcia Kiyomi; BARBEIRO, Denise Frediani; BARBEIRO, Hermes Vieira; HAMASAKI, Mike Yoshio; COELHO NETO, Guilherme Tude; MACHADO, Marcel Cerqueira Cesar; SILVA, Fabiano Pinheiro daAcute pancreatitis is a life-threatening situation, frequently associated with uncontrolled local and systemic inflammation, and aging is associated with a worst prognosis. Antimicrobial peptides are ancient molecules that belong to innate immunity, produced by epithelial and immune cells, and are able to trigger a myriad of effector responses. We have hypothesized that antimicrobial peptides could play an important role during serious pancreatic injury. To investigate our hypothesis, alpha-defensin-5, alpha-defensin-7 and CRAMP gene expression levels were measured in the intestinal tissue of old and young rats submitted to chemical pancreatic damage. We found significantly higher levels of alpha-defensin-5 and alpha-defensin-7, but not CRAMP, in the samples from old mice. This increase was not associated with a worse systemic inflammatory response. We conclude that alpha-defensins may have a pivotal role during acute pancreatitis and that the elderly develops a more severe local, but not systemic inflammatory process.
- Sequential hemodynamic assessment in aortic valve insufficiency in rats(2014) KOIKE, Marcia Kiyomi; MATSUBARA, Beatriz Bojikian; MATSUBARA, Luiz Shiguero; FRIMM, Clovis de CarvalhoOBJECTIVE: In animal models of aortic insufficiency, the right common carotid artery is typically used to damage valve leaflets; this strategy impedes subsequent assessments of left ventricular hemodynamics. The present study aimed to establish an alternative technique that would allow subsequent left ventricular catheterization to monitor sequential hemodynamics in rats with aortic insufficiency. METHOD: The right internal and external carotid artery branches were dissected. The internal branch was temporarily occluded. The external branch was also occluded, and the proximal, patent segment was catheterized. Via the catheter, ventricular hemodynamic evaluations were performed before and after incurring leaflet damage. The catheter was removed, and the right external branch was permanently ligated. The temporary right internal carotid occlusion was released, and blood flow was re-established. After four weeks, left ventricular hemodynamic measurements were performed from the right common carotid artery. RESULTS: Four weeks after the establishment of aortic insufficiency, left ventricular hemodynamic parameters showed a classic chronic hemodynamic pattern, similar to that observed in patients with chronic or compensated aortic insufficiency. Systolic blood pressure was elevated and pulse pressure was increased. CONCLUSION: This new method of carotid artery catheterization permitted two sequential, distinct hemodynamic measurements, in experimental model of aortic valve insufficiency.
- Ischemic Preconditioning and Atenolol on Lung Injury After Intestinal Ischemia and Reperfusion in Rats(2014) BONSERVIZI, W. G. S.; KOIKE, M. K.; SAURIM, R.; FELIX, G. A. A.; SILVA, S. M. da; MONTERO, E. F. S.; TAHA, M. O.The aim of this study was evaluate the beta blocker atenolol (AT) and ischemic preconditioning (IPC) strategies for tissue protection against systemic effects of intestinal ischemia (I) and reperfusion (R) injury. Forty-two rats were pretreated with AT (1.5 mg . kg(-1)), 0.9% saline solution (SS; 0.1 mL), or IPC and then subjected to prolonged occlusion of the superior mesenteric artery for 60 minutes leading to I followed or not by 120 minutes of R, according to the group. For IPC, 5 minutes of I prior to 10 minutes of R were established. After this process of I or I-R, the right lung of each animal was adequately prepared for staining with hematoxylin and eosin and subsequent histologic analysis for quantification of inflammatory infiltrate was done. The left lung was frozen and prepared for assessment of oxidative stress by the quantification of thiobarbituric acid-reactivity substances (TBARS). Histologic analysis showed an important inflammatory infiltrate in the I-R + SS (I-R + SS 4.5), which was significantly (P < .05) reduced by IPC (I-R + IPC = 3.0) or AT (I-R + AT = 3.0). Likewise, the TBARS levels were decreased by both strategies (I-R + SS = 0.63; I-R + IPC = 0.23; I-R + AT = 0.38; P < .05). Our results showed that AT and IPC attenuate pulmonary lesions caused by intestinal I and R process.
- N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats(2014) AMORIM, Elivania Marques Gomes de; DAMOUS, Luciana Lamarao; DURANDO, Maria Clara Silva; SARAIVA, Marcia Viviane Alves; KOIKE, Marcia Kiyomi; MONTERO, Edna Frasson de SouzaPURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC. METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) - treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4th or 15th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test. RESULTS: At 4th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation. CONCLUSION: In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle.
- Heart injury following intestinal ischemia reperfusion in rats is attenuated by association of ischemic preconditioning and adenosine(2014) MONTERO, Micaela Frasson; SAURIM, Rafael; BONSERVIZI, Wesley Guedes Sava; KOIKE, Marcia Kiyomi; TAHA, Murched OmarPURPOSE: To investigate the effect of ischemic preconditioning (IPC) and adenosine as strategies to protect cardiac injury caused by intestinal IR in rats, based on increasing in adenosine bioavailability and improvement of cell energy state by IPC. METHODS: Male Wistar rats were submitted to 60 minutes of intestinal ischemia and 120 minutes of reperfusion. Intravenous injections of saline or Adenosine (AD) was administered five minutes before ischemia, five minutes before reperfusion and after 55 minutes reperfusion. Cardiac samples were obtained, fixed in formalin solution, embedded in paraffin, and sections of 5 mu m were stained by hematoxylin-eosin. Histological analysis of myocardium was performed according occurrence of necrosis signs: piknosis, band contraction, eosinophilic cytoplasm, karyorrhexis and vacuolization (score - zero to 5). RESULTS: The groups submitted to ischemia alone (I=4.0), and reperfusion (IR=4.5) showed highest level of lesion compared to the others (I+IPC=3.3, IR+IPC=3.6, I+AD=3.0, IR+AD=3.8). The most interesting result was association of IPC and AD in IR model (IR+IPC+AD=1.2, p=0.002), showing preservation of the heart tissue, with fibers showing typical cross-striations and nuclei characteristics. Rare and small areas of tissue necrosis was observed and suggestion of capillaries congestion. CONCLUSION: Intestinal ischemia reperfusion promotes cardiac tissue injury. Ischemic preconditioning in association with adenosine is an efficient strategy to protect the heart against ischemia and reperfusion injury.
- The effect of n-acetylcysteine on hepatic histomorphology during hypothermic preservation(2014) RISSO, Patricia Sayuri; KOIKE, Marcia Kiyomi; ABRAHAO, Marcos de Souza; FERREIRA, Natalie Chaves; MONTERO, Edna Frasson de SouzaPURPOSE: To evaluate the NAC effects on liver hypothermic preservation at different time intervals. METHODS: For this, we used livers of male Wistar rats weighing between 250 and 300g, undergoing preservation in Ringer solution at 4 degrees C for up to 24 hours. Tissue samples were obtained at four moments of preservation for histological examination by hematoxylin and eosin staining: T0 = beginning of preservation, T12 = 12 hours, T18 = 18 hours and T24 = 24 hours. Will be analyzed vacuolation, hepatic apoptosis by optical microscopy and parenchymal. RESULTS: The results showed a progressive increase in hepatic injury in both groups and showed that NAC was effective at T0. The parenchyma preservation was better in the NAC group and no difference when vacuolization of the cells. CONCLUSION: Hypothermic preservation, over time, causes changes in the hepatic parenchyma with increased apoptosis, loss of architecture, vacuolization, culminating in severe injury. The administration of N-acetylcysteine protects against preservation liver injury.
conferenceObject Increased Bacterial Translocation in Aging Animals Is Not Related to Decreased Intestinal Antimicrobial Peptide Expression in Acute Pancreatitis(2014) CUNHA, Debora G.; SILVA, Fabiano Pinheiro da; BARBEIRO, Denise F.; KOIKE, Marcia K.; MACHADO, Marcel C.; VELASCO, Irineu T.conferenceObject Interesterified Fats Induce Nonalcoholic Steatohepatitis or Enhance the Adipose Tissue and Adipocyte Hypertrophy(2014) LAVRADOR, Maria Silvia F.; AFONSO, Milessa S.; KOIKE, Marcia; BOMBO, Renata P.; CINTRA, Dennys E.; NUNES, Valeria S.; CATANOZI, Sergio; NAKANDAKARE, Edna R.; LOTTENBERG, Ana Maria- Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats(2014) FRIAS NETO, Carlos Alberto da Silva; KOIKE, Marcia Kiyomi; SAAD, Karen Ruggeri; SAAD, Paulo Fernandes; MONTERO, Edna Frasson de SouzaPURPOSE: To evaluate effects of ischemic preconditioning and Cilostazol on muscle ischemia-reperfusion injury. METHODS: Male Wistar rats were submitted to muscle ischemic and reperfusion injury (4h of the left common iliac artery occlusion followed by 1h of reperfusion). Five experimental groups were constituted: Control group (n=4); Ischemia-Reperfusion (IR, n=5); Ischemic preconditioning group (IP, n=6); Ischemia-Reperfusion group treated with cilostazol (IRCi, n=6) and Ischemic preconditioning group treated with cilostazol (IPCi, n=6). At the end, left gracile muscle was removed and embedded in paraffin. Histopathology, neutrophil infiltration, myocyte necrosis and edema were analyzed. RESULTS: When compared with the control group, IR group showed increased neutrophil infiltration, severe necrosis and edema. There was significant difference between myocytes necrosis of IR group and IP group. There was no difference between the histopathological changes between IP, IRCi and IPCi groups. CONCLUSIONS: The model of IR caused severe muscle injury in the rat hind limb and ischemic preconditioning has a protective effect, reducing myocyte necrosis, however, treatment with cilostazol and also the association between cilostazol and preconditioning has no protective effect on the skeletal muscle subjected to ischemia and reperfusion injury.