MARCIA KIYOMI KOIKE
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Clínica Médica, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
LIM/51 - Laboratório de Emergências Clínicas, Hospital das Clínicas, Faculdade de Medicina
5 resultados
Resultados de Busca
Agora exibindo 1 - 5 de 5
- Synergy of Rapamycin and Cyanoacrylate in Reducing Intimal Hyperplasia(2019) KOIKE, Marcia Kiyomi
- Factors Associated With Medication Adherence In Elderly Retired Outpatients In Sao Paulo, Brazil(2019) VALASSI, Juliana Martins Ribeiro; CARVAS JUNIOR, Nelson; SHIRASSU, Mirian Matsura; PAULA, Kaleo Eduardo de; ATKINSON, Elena R.; KOIKE, Marcia KiyomiObjective: To evaluate medication adherence and associated socioeconomic factors in elderly Brazilians. Methodology: This observational study was conducted with 159 elderly retired in an outpatient clinic in the city of Sao Paulo. Treatment adherence was assessed with the questions from the Morisky Green Levine Medication Adherence Questionnaire, and medications were classified using the Anatomical Therapeutic Chemical system. Statistical tests and adjusted Poisson regression models were used to analyze variables. Results: The study population was mostly female (67.5%), had an average age of, and took an average of 6.5 medications per day. The most commonly used drugs were agents acting on the renin-angiotensin system (67.9%), statins (62.3%), antithrombotic agents (48.4%), and biguanides (37.1%) for the treatment of hypertension (76.7%), dyslipidemia (54.1%), and diabetes (47.8%). The rate of adherence was below 60% in the groups of participants that were analyzed except for the high household income category, which had a rate of 75.8%. Conclusion: Medication adherence among the elderly was low in all categories except for the high household income category, a relevant finding that will help to understand medication adherence patterns in elderly Brazilians.
- Interesterified Fats Induce Deleterious Effects on Adipose Tissue and Liver in LDLr-KO Mice(2019) LAVRADOR, Maria Silvia Ferrari; AFONSO, Milessa Silva; CINTRA, Dennys Esper; KOIKE, Marcia; NUNES, Valeria Sutti; DEMASI, Marina; LIN, Chin Jia; BEDA, Lis Mie Masuzawa; GIOIELLI, Luiz Antonio; BOMBO, Renata de Paula Assis; MACHADO, Roberta Marcondes; CATANOZI, Sergio; NAKANDAKARE, Edna Regina; LOTTENBERG, Ana MariaInteresterified fats are being widely used by the food industry in an attempt to replace trans fatty acids. The effect of interesterified fats containing palmitic or stearic acids on lipid metabolism and inflammatory signaling pathways in adipose and hepatic tissues was evaluated. Male LDLr-KO mice were fed a high-fat diet containing polyunsaturated (PUFA), palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR), or stearic interesterified (STEAR INTER) fats for 16 weeks. The expression of genes and protein levels involved in lipid metabolism and inflammatory processes in liver and white adipose tissue was determined by quantitative RT-PCR and by Western blot, respectively. The infiltration of inflammatory cells in hepatic and adipose tissues was determined by eosin and hematoxylin, while liver collagen content was determined by Sirius Red staining. Both interesterified fats increased liver collagen content and JNK phosphorylation. Additionally, the STEAR INTER group developed nonalcoholic steatohepatitis (NASH) associated with higher neutrophil infiltration. PALM INTER induced adipose tissue expansion and enlargement of adipocytes. Furthermore, PALM INTER triggered increased IKK phosphorylation and TNF protein content, conditions associated with the upstream activation of the NFkB signaling pathway. STEAR INTER induced NASH, while PALM INTER triggered hepatic fibrosis and adipocyte hypertrophy with inflammatory response in LDLr-KO mice.
- Hypertonic saline and pentoxifylline enhance survival, reducing apoptosis and oxidative stress in a rat model of strangulated closed loop small bowel obstruction(2019) SCAPINI, Gustavo; RASSLAN, Roberto; CAYUELA, Natalie Chaves; GOES, Miguel Angelo; KOIKE, Marcia Kiyomi; UTIYAMA, Edivaldo Massazo; MONTERO, Edna Frasson de Souza; RASSLAN, SamirOBJECTIVES: Intestinal obstruction has a high mortality rate when therapeutic treatment is delayed. Resuscitation in intestinal obstruction requires a large volume of fluid, and fluid combinations have been studied. Therefore, we evaluated the effects of hypertonic saline solution (HS) with pentoxifylline (PTX) on apoptosis, oxidative stress and survival rate. METHODS: Wistar rats were subjected to intestinal obstruction and ischemia through a closed loop ligation of the terminal ileum and its vessels. After 24 hours, the necrotic bowel segment was resected, and the animals were randomized into four groups according to the following resuscitation strategies: Ringer's lactate solution (RL) (RL-32 ml/kg); RL+ PTX (25mg/kg); HS+ PTX (HS, 7.5%, 4 ml/kg), and no resuscitation (IO-intestinal obstruction and ischemia). Euthanasia was performed 3 hours after resuscitation to obtain kidney and intestine samples. A malondialdehyde (MDA) assay was performed to evaluate oxidative stress, and histochemical analyses (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling [TUNEL], Bcl-2 and Bax) were conducted to evaluate kidney apoptosis. Survival was analyzed with another series of animals that were observed for 15 days. RESULTS: PTX in combination with RL or HS reduced the MDA levels (nmol/mg of protein), as follows: kidney IO=0.42; RL=0.49; RL+ PTX=0.31; HS+ PTX=0.34 (p<0.05); intestine: IO=0.42; RL=0.48; RL+ PTX=0.29; HS + PTX=0.26 (p<0.05). The number of labeled cells for TUNEL and Bax was lower in the HS+ PTX group than in the other groups (p<0.05). The Bax/Bcl-2 ratio was lower in the HS+ PTX group than in the other groups (p<0.05). The survival rate on the 15 th day was higher in the HS+ PTX group (77%) than in the RL+ PTX group (11%). CONCLUSION: PTX in combination with HS enhanced survival and attenuated oxidative stress and apoptosis. However, when combined with RL, PTX did not reduce apoptosis or mortality.
- Short-Term Effects of Sepsis and the Impact of Aging on the Transcriptional Profile of Different Brain Regions(2019) HAMASAKI, Mike Yoshio; SEVERINO, Patricia; PUGA, Renato David; KOIKE, Marcia Kiyomi; HERNANDES, Camila; BARBEIRO, Hermes Vieira; BARBEIRO, Denise Frediani; MACHADO, Marcel Cerqueira Cesar; REIS, Eduardo Moraes; SILVA, Fabiano Pinheiro daAmong the clinical manifestations observed in septic patients, sepsis-associated encephalopathy (SAE) is probably the most obscure and poorly explored. It is well established, however, that SAE is more prevalent in aged individuals and related to a worse outcome. In this context, we decided to investigate the acute effects of sepsis, induced by cecal ligation and puncture (CLP), on the cerebral transcriptional profile of young and old rats. The idea was to highlight important signaling pathways possibly implicated in the early stages of SAE. Global gene expression analysis of three different brain regions (hippocampus, cerebellum, and cortex) indicated a relatively small interference of sepsis at the transcriptional level. Cerebellum tissue was the least affected by sepsis in aged rats. The increased expression of S100a8, Upp1, and Mt2a in all three brain regions of young septic rats indicate that these genes may be involved in the first line of response to sepsis in the younger brain. On the other hand, altered expression of a network of genes involved in sensory perception of smell in the cortex of aged rats, but not in young ones, indicates an earlier disruption of cortex function, possibly more sensitive to the systemic inflammation. The expression of S100a8 at the protein level was confirmed in all brain regions, with clear-up regulation in septic aged cortex. Taken together, our results indicate that the transcriptional response of the central nervous system to early sepsis varies between distinct brain regions and that the cortex is affected earlier in aged animals, in line with early neurological manifestations observed in older patients.