NATALIA GARCIA
Projetos de Pesquisa
Unidades Organizacionais
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
4 resultados
Resultados de Busca
Agora exibindo 1 - 4 de 4
- SHH pathway in uterine mesenchymal tumors(2014) GARCIA, Natalia; SOUZA, Faila C.; BOZZINI, Nilo; BAIOCCHI, Glauco; CUNHA, Isabela W.; SOARES, Fernando A.; BARACAT, Edmund C.; CARVALHO, Katia C.
conferenceObject GLI transcription factors involvement in leiomyoma genesis(2016) GARCIA, Natalia; ANJOS, Laura; BOZZINI, Nilo; MACIEL, Gustavo; BARACAT, Edmund; CARVALHO, Katia- May Sonic Hedgehog proteins be markers for malignancy in uterine smooth muscle tumors?(2016) GARCIA, Natalia; BOZZINI, Nilo; BAIOCCHI, Glauco; CUNHA, Isabela Werneck da; MACIEL, Gustavo Arantes; SOARES JUNIOR, Jose Maria; SOARES, Fernando Augusto; BARACAT, Edmund Chada; CARVALHO, Katia CandidoSeveral studies have demonstrated that the Sonic Hedgehog signaling pathway (SHH) plays an important role in tumorigenesis and cellular differentiation. We analyzed the protein expression of SHH pathway components and evaluated whether their profile could be useful for the diagnosis, prognosis, or prediction of the risk of malignancy for uterine smooth muscle tumors (USMTs). A total of 176 samples (20 myometrium, 119 variants of leiomyoma, and 37 leiomyosarcoma) were evaluated for the protein expression of the SHH signaling components, HHIP1 (SHH inhibitor), and BMP4 (SHE target) by immunohistochemistry. Western blot analysis was performed to verify the specificity of the antibodies. We grouped leiomyoma samples into conventional leiomyomas and unusual leiomyomas that comprise atypical, cellular, mitotically active leiomyomas and uterine smooth muscle tumors of uncertain malignant potential. Immunohistochemical analysis showed that SMO, SUFU, GLIL GLI3, and BMP4 expression gradually increased depending on to the histologic tissue type. The protein expression of SMO, SUFU, and GLI1 was increased in unusual leiomyoma and leiomyosarcoma samples compared to normal myometrium. The inhibitor HHIP1 showed higher expression in myometrium, whereas only negative or basal expression of SMO, SUFU, GLIL and GLI3 was detected in these samples. Strong expression of SHE was associated with poorer overall survival. Our data suggest that the expression of SHH proteins can be useful for evaluating the potential risk of malignancy for USMTs. Moreover, GLI1 and SMO may serve as future therapeutic targets for women with USMTs.
- Smoothened a new perspective for uterine leiomyoma treatment(2017) GARCIA, Natalia; ANJOS, Laura Gonzalez; MAFFAZIOLI, Giovana De Nardo; BOZZINI, Nilo; BARACAT, Edmund Chada; CARVALHO, Katia Candido