ALFREDO ELIAS GILIO
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Pediatria, Faculdade de Medicina - Docente
PAINT-62, Hospital Universitário
PAINT-62, Hospital Universitário
9 resultados
Resultados de Busca
Agora exibindo 1 - 9 de 9
- Health worker recommended vaccination rates among medical students in Brazil(2021) LEME, Mateus Deckers; GILIO, Alfredo EliasHealth workers are a risk population for many infectious diseases, which leads to a number of vaccines being routinely recommended for health care staff. Medical students are also prone to such hazards. This study accesses undergraduate medicine students' compliance to recommended health-staff vaccination, and their reasons for noncompliance. Method: An online questionnaire was sent to all undergraduates in a major public medical school in Brazil, asking about vaccination status to Hepatitis B, Measle-mumps-rubella, Varicella, Pertussis and Influenza, and reasons in case of noncompliance Results: 146 students answered the questionnaire, (response rate 14,6%). Overall vaccination status showed 74,7% of students with incomplete vaccination in some way, with an increase in vaccination status toward the end of the course. The highest noncompliance rates were Pertussis (49,3%), Varicella (47,3%) and Influenza (30,1%) vaccines. The vaccine with the lowest noncompliance rate was measles (9,6%). During the course, the greatest increases in adequate vaccination status were Hepatitis B, from 53,2% in first-years to 93,2% by the end sixth year (chi-sq 21, p < 0,0001), and Influenza, from 48,9% to 91,5% (chi-sq 22,5, p < 0,00009). Main reasons given not to vaccinate were vaccination hesitancy for influenza and varicella (respectively 61% and 46%), and lack of awareness of the need to vaccinate for pertussis (53%). Conclusions: Overall vaccine coverage in medical students in Brazil is still far from optimal. There is a markedly high level of vaccine hesitancy and unawareness of need to vaccinate for some diseases, particularly pertussis and influenza. (C) 2021 Published by Elsevier Ltd.
- Number Needed to Treat in Fluconazole Prophylaxis in the Neonatal Intensive Care Unit(2011) SHIEH, Huei Hsin; IBIDI, Silvia Maria; GILIO, Alfredo Elias
- THE BACTERIAL MENINGITIS SCORE TO DISTINGUISH BACTERIAL FROM ASEPTIC MENINGITIS IN CHILDREN FROM SAO PAULO, BRAZIL(2013) MEKITARIAN FILHO, Eduardo; HORITA, Sergio Massaru; GILIO, Alfredo Elias; ALVES, Anna Claudia Dominguez; NIGROVIC, Lise E.In a retrospective cohort of 494 children with meningitis in Sao Paulo, Brazil, the Bacterial Meningitis Score identified all the children with bacterial meningitis (sensitivity 100%, 95% confidence interval: 92-100% and negative predictive value 100%, 95% confidence interval: 98-100%). Addition of cerebrospinal fluid lactate to the score did not improve clinical prediction rule performance.
- High Frequency of Staphylococcus Saprophyticus Urinary Tract Infections Among Female Adolescents(2015) LO, Denise Swei; SHIEH, Huei Hsin; BARREIRA, Eliane Roseli; RAGAZZI, Selma Lopes Betta; GILIO, Alfredo EliasStaphylococcus saprophyticus is a rarely reported agent of urinary tract infection (UTI) in the pediatric population. In our retrospective 3-year study, S. saprophyticus comprised 24.5% of 106 isolates of UTIs in female adolescents 12-15 years of age who attended an emergency department. Clinicians should be aware of the high prevalence of this etiology when empirically treating UTIs in female adolescents.
- Multisystem Inflammatory Syndrome Associated With COVID-19 With Neurologic Manifestations in a Child: A Brief Report(2020) PAULIS, Milena De; OLIVEIRA, Danielle Bruna Leal; VIEIRA, Rodolfo P.; PINTO, Isabella Coutinho; MACHADO, Rafael Rahal Guaragna; CAVALCANTI, Mariana Pauferro; SOARES, Camila Pereira; ARAUJO, Ananda Medeiros Pereira de; ARAUJO, Danielle Bastos; BACHI, Andre Luis Lacerda; LEAL, Fabyano Bruno; DORLASS, Erick Gustavo; GILIO, Alfredo Elias; DURIGON, Edison Luiz; BARREIRA, Eliane RoseliAlthough first considered a benign infection, recent studies have disclosed severe and potentially lethal inflammatory manifestations of COVID-19 in children. We report the case of a 4-year-old child with a post-infectious multisystem inflammatory syndrome associated with COVID-19, with a Kawasaki-like shock and prominent neurologic features, for whom a cytokine storm and reduced brain-derived neurotrophic factor were well documented.
- Frequency of Community-acquired Methicillin-resistant Staphylococcus aureus in Pediatric Population in a General Hospital in Sao Paulo, Brazil, Over 5 Years(2019) PENTEADO, Fernando Domingues; TUBERO, Thais Zenero; HEIN, Noely; GILIO, Alfredo EliasWe retrospectively evaluated the frequency of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections in children at a general hospital in Sao Paulo, Brazil, from 2011 to 2015. Of 64 patients with confirmed S. aureus infection, 11 (17.2%) had CA-MRSA. CA-MRSA infections in Brazil, and in particular those with a severe presentation, should not be overlooked.
- A study of acceptance and hesitation factors towards tetanus, diphtheria, and acellular pertussis (Tdap) and influenza vaccines during pregnancy(2023) QUILES, Raquel; LEME, Mateus Deckers; LO, Denise Swei; GILIO, Alfredo EliasIntroduction: Vaccination of pregnant women with tetanus, diphtheria, and acellular pertussis (Tdap) and influenza vaccines is desirable to reduce neonatal and maternal morbidity and mortality. However, vaccine coverage rates and acceptance are frequently below recommended rates.Objectives: To ascertain Tdap and influenza vaccine coverage rates in our population and to study the reasons behind sub-optimal rates.Method: A survey was submitted to pregnant or in their puerperium women at the University Hospital of Sa & SIM;o Paulo University. Data were obtained during two consecutive influenza seasons (2017-2018), and vaccination was verified through vaccination chart checking. Respondents were classified according to their status as ""Received Tdap"" and ""Didn't receive Tdap"", and as ""Know"" or ""Doesn't know"" regarding their awareness of Tdap safety during pregnancy and protective effect on the newborn. Vaccine uptake and personal awareness of vaccination status were compared among these groups for Tdap and influenza vaccines.Results: In a studied sample of 207 patients (representative of the whole), coverage rates for Tdap and influenza vaccines were respectively 85.5% and 95.2%. Additionally, 84.5% received both vaccines. There was no vaccine refusal for Tdap and only 0.5% for influenza. For either Tdap or influenza vaccines, the main reason for not vaccinating was a lack of knowledge/information. Factors associated with not vaccinating Tdap during pregnancy were lower number of prenatal visits, being unemployed or freelance worker, not being aware of vaccine safety or its benefits for the baby, not being oriented by the doctor to be vaccinated, not being aware of personal vaccination status, and not having been vaccinated for influenza.Conclusion: While influenza vaccination coverage during pregnancy was ideal, Tdap rates were below recommended values. Significant factors associated with better coverage for Tdap during pregnancy included being employed and not being self-employed, (not yet reported in the Americas) and being aware of personal vaccination status.
- Macrophage profile and homing into breast milk in response to ongoing respiratory infections in the nursing infant(2020) ZHENG, Yingying; CORREA-SILVA, Simone; SOUZA, Eloisa Correa de; RODRIGUES, Regina Maria; FONSECA, Fernanda A. Macaferri da; GILIO, Alfredo Elias; CARNEIRO-SAMPAIO, Magda; PALMEIRA, PatriciaStudies have shown that immune components of human milk can be changed during an infection in the nursing infant. Macrophages are abundant in human milk and they are classified into inflammatory (CD16(-)) and noninflammatory (CD16(+)) subsets. This study investigated CD16(+) and CD16(-) macrophage homing into breast milk in response to ongoing infections in nursing infants. Peripheral blood and mature milk were collected from 33 healthy mothers of nursing infants with respiratory infections (Group I) and from 26 healthy mothers of healthy nursing infants (Group H). Blood and milk total, CD16(-) and CD16(+) monocyte (Mo)/macrophage (M phi) subsets, respectively, and CCR2 and CX3CR1 expression and cytokine levels were analyzed by flow cytometry. CCL2 and CX3CL1 were quantified by ELISA and cytokines by flow cytometry in serum and milk. There was an increase of total and CD16(+) M phi, and, also a decrease of CD16- M phi frequencies in maternal milk from Group I compared to Group H, but absolute numbers analyses showed higher numbers of all subpopulations of milk M phi in Group I compared to Group H. Higher numbers of CX3CR1(+)CD16(+) and double-staining of CCR2 and CX3CR1 in both CD16(+) and CD16(-) cells were observed in milk during infant infection, which weren't observed in the blood. CCR2 expression was hardly found in milk CD16(-) M phi in both groups. CCL2 and CX3CL1 were both higher in milk than in blood from both groups, but Group I showed higher levels of these chemokines in milk than Group H. Breast milk showed higher IL-6 and IL-8 concentrations than serum, and infant infection caused an increase in these cytokines only in milk. Our findings suggest that milk M phi profiles are different from blood Mo, and the ongoing infection in the nursing infant could change milk M phi to a more anti-inflammatory profile compared to that in the healthy group, possibly as an additional strategy of infant protection.
- Serotype distribution of Streptococcus pneumoniae isolated from patients with invasive pneumococcal disease in Brazil before and after ten-pneumococcal conjugate vaccine implementation(2013) SANTOS, Silvia R. dos; PASSADORE, Lilian F.; TAKAGI, Elizabeth H.; FUJII, Cristiane M.; YOSHIOKA, Cristina R. M.; GILIO, Alfredo E.; MARTINEZ, Marina B.The ten-pneumococcal conjugate vaccine (PCV10) was introduced into the national immunization program for childhood vaccination schedules by the Brazilian Health Public Service in March 2010. The aim of this study was to compare Streptococcus pneumoniae serotype distribution, antibiotic resistance patterns, and potential coverage before (January 2006-June 2010) and after (July 2010-September 2012) PCV10 introduction. The incidence of invasive pneumococcal disease (IPD), patient demographics, and disease characteristics were recorded. This study was conducted at the University Hospital of Sao Paulo University in Brazil from January 2006 to September 2012. Serotyping was performed using multiplex PCR typing, and antimicrobial sensitivity by Clinical and Laboratory Standards Institute (CLSI). A total of 259 S. pneumoniae strains were isolated from patients with IPD. The ages of the patients ranged from 3 months to 95 years old. The strains were isolated from cerebrospinal fluid, pleural fluid, and blood. The incidence of IPD among patients at HU-USP changed after the introduction of PCV10. The overall incidence of IPD was 3.42 cases per 1000 admissions in the vaccine pre- implementation period and of 2.99 cases per 1000 admissions in the vaccine post-implementation period. The incidence of IPD among children<2 y.o. attended at HU-USP changed significantly after the introduction of PCV10, from 20.30 to 3.97 of incidence. The incidence of PCV10- serotypes decrease from 16.47 to 0.44 in the same age, before and after PC10 implementation, respectively. Moreover, it was possible to realize the sensitivity to penicillin among isolates increased significantly in the post-vaccine period. Data from this study suggest that PCV10 contributed to decrease with PID rate among children less than 2 y.o. The resistance rate among pneumococcal isolates also could be observed since serotypes with greater resistance to beta lactam antibiotics were not easily isolated after vaccination.(C) 2013 Elsevier Ltd. All rights reserved.