RAQUEL LEAO ORFALI
Projetos de Pesquisa
Unidades Organizacionais
Instituto Central, Hospital das Clínicas, Faculdade de Medicina - Médico
LIM/56 - Laboratório de Investigação em Dermatologia e Imunodeficiências, Hospital das Clínicas, Faculdade de Medicina
LIM/56 - Laboratório de Investigação em Dermatologia e Imunodeficiências, Hospital das Clínicas, Faculdade de Medicina
14 resultados
Resultados de Busca
Agora exibindo 1 - 10 de 14
conferenceObject A 25-year overview of the atopic dermatitis outpatient clinic at the University of Sao Paulo Medical School, Brazil(2014) ZANIBONI, M. C.; ORFALI, R. L.; TAKAOKA, R.; ISHIZAKI, A. S.; AOKI, V.conferenceObject IgG from atopic dermatitis patients induces IL-17 and IL-10 production in infant intra-thymic TCD4 and TCD8 cells(2018) SGNOTTO, F. R.; OLIVEIRA, M. G.; LIRA, A. A. L.; INOUE, A. H. S.; TITZ, T. O.; ORFALI, R. L.; BENTO-DE-SOUZA, L.; SATO, M. N.; AOKI, V.; DUARTE, A. J. S.; VICTOR, J. R.conferenceObject Atopic dermatitis in adults: Augmented circulating IgG4 and IgE antibodies against Staphylococcus aureus enterotoxin B(2013) ORFALI, R. L.; SATO, M. N.; SANTOS, V. G. Dos; TITZ, T. O.; DUARTE, A. S.; TAKAOKA, R.; AOKI, V.The aim of this study was to evaluate the profile of anti-Staphylococcus aureus enterotoxin B (SEB) antibody (Ab) response in adults with AD. We selected 38 patients diagnosed as AD (Hanifin & Rajka's criteria), aged between 18 and 65, and 26 healthy controls (HC), aged between 20 and 47 years. Severity of the disease was established according to EASI (Eczema Area and Severity Index) and patients were graded as mild (28%), moderate (58%) and severe (14%). Sera were assessed for IgG subclasses, IgA, IgM and IgE against SEB by ELISA. The humoral response in AD patients to SEB showed elevated circulating IgE and IgG4 levels (p < 0.05 and p δ 0.001, respectively), and decreased IgA and IgM levels (p < 0.05). Severity of atopic dermatitis was related with low IgG1 and IgG3 levels (p < 0.05), and high IgE antibody response (p < 0.05) to SEB. Interestingly, absence of IgE and IgG1 response to SEB was seen in some AD patients (26.3% and 18.4% respectively). In AD, the immunoglobulin-related subtype Th2 lymphocytes, such as IgE and IgG4, appear to be relevant in the response to superantigens. These results emphasize an altered pattern of Ab response to SEB in AD, which is impaired according the disease severity.- Evaluating chronic pruritus prevalence, characteristics and effects on atopic dermatitis patients from a referral university hospital in Sao Paulo, Brazil: a cross-sectional study(2023) SOARES, Georgia Biazus; ORFALI, Raquel Leao; AVERBACH, Beatriz Lacerda; YOSIPOVITCH, Gil; AOKI, Valeria
conferenceObject Staphylococcus aureus enterotoxins modulate IL-22-secreting cells in adults with atopic dermatitis(2018) ORFALI, R. L.; OLIVEIRA, L. M. S.; LIMA, J. F.; CARVALHO, G. C.; RAMOS, Y. A. L.; PEREIRA, N. Z.; VIEIRA, N. P.; ZANIBONI, M. C.; SATO, M. N.; AOKI, V.conferenceObject Impact of climate change on atopic dermatitis: A systematic search and review by the International Eczema Council(2023) WANG, S.; STEFANOVIC, N.; ORFALI, R. L.; AOKI, V.; BROWN, S.; DHAR, S.; EICHENFIELD, L. F.; FLOHR, C.; HA, A.; MORA, C.; MURASE, J.; ROSENBACH, M.; THYSSEN, J.; WEI, M. L.; IRVINE, A. D.; ABUABARA, K.conferenceObject Atopic dermatitis in adults: Association of impaired of CD38 and CD69 expression and eosinophilia(2014) TITZ, Tiago de Oliveira; LOLLO, Camila de; SATO, Maria Notomi; ORFALI, Raquel Leao; AOKI, Valeria; SANTOS, Vanessa Goncalves dosconferenceObject Phenotypic profile of CLA plus natural killer cells in adults with atopic dermatitis(2018) ORFALI, R. L.; LIMA, J. F.; CARVALHO, G. C. C.; RAMOS, Y. A. L.; DUARTE, A. J. S.; SATO, M.; AOKI, V.conferenceObject Adult atopic dermatitis: Evaluation of TH17 and TH22 cytokines in peripheral blood mononuclear cells induced by staphylococcal enterotoxins A and B(2012) ORFALI, Raquel Lean; RIVITTI, Evandro; SATO, Maria Notomi; TAKAOKA, Roberto; AOKI, ValeriaObjective: Evaluation of interleukins (IL) 17, 22, and 23 induced by Staphylococcus aureus enterotoxin stimulation in peripheral blood mononuclear cells (PBMC) of adults with atopic dermatitis. Background: Atopic dermatitis (AD) is a chronic, inflammatory disease with a high prevalence and complex etiopathogenesis.S aureusis present in 80% to 100% of AD patients, and secretes exotoxins that might relate to its pathogenesis. TH17 has been described as playing a major role in inflammatory diseases with a close relationship to bacterial pathogens, as well as TH22 in modulating the immunopathogenesis of some skin diseases. Methods: Thirty-eight AD patients (mean age, 28.55), 19 female and 19 male, and 40 healthy controls (mean age, 34.1), 21 females and 19 males (without personal or family history of atopy and negative prick-test) were selected. Hanifin and Rajka’s criteria were used to diagnose AD. Disease severity was established according to EASI (Eczema Area and Severity Index). IL-17, IL-22, and IL-23 production from PBMC after staphylococcal enterotoxins A (SEA) and B (SEB), and phytohemaglutinin (PHA) stimuli and IL-22 serum levels were measured by ELISA. Results: In AD patients, there was increased IL-22 levels in sera as well as in vitro secretion by PBMC after SEA and SEB stimuli, when compared to healthy controls. No correlation between IL-22 levels and EASI was observed (P ≤ .05). Conclusion: These findings suggest an involvement of TH22 subtype in the pathogenesis of adults with AD, especially because of its link to S aureus enterotoxins.conferenceObject Evaluation of circulating chemokines in patients with inflammatory dermatoses (atopic dermatitis and psoriasis) and autoimmune blistering diseases(2014) ANZAI, Alessandra; HONG, Bomi; SZWAKO, Natasha; ORFALI, Raquel; AOKI, Valeria