PAULO CESAR SERAFINI
Projetos de Pesquisa
Unidades Organizacionais
Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina - Docente
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
LIM/58 - Laboratório de Ginecologia Estrutural e Molecular, Hospital das Clínicas, Faculdade de Medicina
18 resultados
Resultados de Busca
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bookPart Investigação básica do casal infértil(2016) MIYADAHIRA, Eduardo Hideki; BIANCHI, Paulo Homem de Mello; SERAFINI, Paulo César- Blind aspiration biopsy versus a guided hysteroscopic technique for investigation of the endometrium in infertile women(2016) EJZENBERG, Dani; SIMOES, Manuel de Jesus; PINHEIRO, Walter; SOARES JUNIOR, Jose Maria; SERAFINI, Paulo Cesar; BARACAT, Edmund ChadaEmbryo implantation failure and recurrent abortion are common indications for endometrial evaluation to determine the implantation window and diagnose endometrial anomalies. There are few research studies comparing the efficacy of different techniques used for endometrial sampling in infertile females during the luteal phase. Likewise, morphometric studies of the endometrium through aspiration biopsy are scant. A cross-sectional study of 30 infertile and 10 fertile females was carried out. The study participants underwent hysteroscopic and aspiration biopsies (pipelle) at the midluteal phase. Computer-assisted morphometric and pathological anatomy analyses were conducted independently by two pathologists blinded to the study. The two endometrial sampling biopsy techniques were compared through morphometric and pathological anatomy analyses using three parameters: a) the amount of material collected for the endometrial studies; b) the scope and origin of sampled materials; and c) the quality of the sample. Both biopsy techniques produced sufficient material for analysis. The directed biopsies yielded higher quality samples from targeted segments of the uterine cavity because samples were homogeneous and had no architectural distortion (p<0.05). Blood was present only in the samples obtained through a Pipelle. Endometritis was detected in 10% of the infertile women. Our findings suggest that hysteroscopic biopsies are superior to blinded aspiration biopsies.
conferenceObject Melatonin action on luteal - granulosa cells in women with marital infertility undergoing in vitro fertilization(2016) MAGANHIN, Carla; CARVALHO, Katia; TURCO, Edson Lo; SERAFINI, Paulo; GARCIA, Natalia; CIPOLLA-NETTO, Jose; SIMOES, Manuel; BARACAT, Edmund; SOARES-JUNIOR, Jose MariabookPart Infertilidade por fator ovulatório(2016) FETTBACK, Paula; BIANCHI, Paulo Homem de Mello; YAMAKAMI, Lucas Yugo Shiguehara; IZZO, Carlos; SERAFINI, Paulo CésarconferenceObject Higher Expression of NOTCH1 Signaling in Endometriotic Lesions Contributes to Progesterone Resistance via Hyper-Methylation of Progesterone Receptor.(2016) SU, Ren-Wei; STRUG, Michael; JOSHI, Niraj; JEONG, Jae-Wook; MIYADAHIRA, Eduardo; SERAFINI, Paulo; FAZLEABAS, AsgerallybookPart Fator tuboperitoneal - obstrução tubária, hidrossalpinge e aderências pélvicas(2016) TOMIOKA, Renato Bussadori; DUARTE FILHO, Oscar Barbosa; KRöGER, Gustavo Burcovschi; RIBEIRO, Sérgio Conti; SERAFINI, Paulo César- Expression of stem cell-related genes in the endometrium and endometriotic lesions: a pilot study(2016) FETTBACK, Paula B. T.; PEREIRA, Ricardo M. A.; ROCHA, Andre M.; SOARES- JR., Jose Maria; SMITH, Gary D.; BARACAT, Edmund C.; SERAFINI, Paulo C.Objective: To compare the expression of stem cell-related genes in the endometrium (END), superficial endometriosis (SE), and deep infiltrating endometriosis (DIE).Study design: We performed a prospective pilot study of six women suffering from SE and DIE who gave consent for laparoscopy surgery, endometrial biopsies, and participation in this study. Quantitative RT-PCR analysis of 84 stem cell-related genes was performed in 18 biopsy samples.Results: A total of 40 of 84 genes were expressed in SE and DIE, but were different from END as follows. Seven genes were over-expressed in SE and 33 genes were under-expressed in DIE compared with END. Two genes were only over-expressed in SE and three genes were only over-expressed in DIE. Six under-expressed genes were exclusively located in SE and one was only located in DIE. The remaining 31 genes were not different among the groups. There was no significant difference in gene expression between SE and DIE samples.Conclusion: Tissue of DIE and SE appears to have similar stem cell-related genes. Nevertheless, there are differences in gene expression between SE and DIE.
bookPart Infertilidade Femina(2016) IZZO, Carlos Roberto; MONTELEONE, Pedro Augusto Araújo; SERAFINI, Paulo Cesar; BARACAT, Edmund ChadaconferenceObject Fertility and Pregnancy Outcomes after Ovarian Stimulation in Five Patients with Congenital Hypopituitarism Treated with GH Since Childhood(2016) CORREA, F. A.; BIANCHI, P. H. M.; FRANCA, M. M.; OTTO, A. P.; RODRIGUES, R. J. M.; I, D. Ejzenberg; SERAFINI, P. C.; ARNHOLD, I. J. P.; MENDONCA, B. B.; CARVALHO, L. R. S.- Outcomes of elective cryopreserved single or double embryo transfers following failure to conceive after fresh single embryo transfer(2016) MONTELEONE, Pedro Augusto Araujo; MIRISOLA, R. J.; GONALVES, S. P.; BARACAT, Edmund C.; SERAFINI, Paulo C.The main adverse effect of IVF is the high multiple pregnancy rate resulting from the transfer of two or more embryos. The objective was to evaluate pregnancy rates in infertile women with a good prognosis who failed to conceive in a fresh elective single embryo transfer (eSET) and had a second cycle with elective double vitrified-warmed embryo transfer (eDFET) compared with elective single vitrified-warmed embryo transfer (eSFET). A total of 142 intracytoplasmic sperm injection cycles using a conventional protocol were evaluated. Good-prognosis patients underwent eSET in a fresh cycle, and those who failed to conceive underwent a second vitrified-warmed embryo transfer: eDFET (n = 102) or eSFET (n = 40). Embryos were transferred and vitrified on day 5 of development. Patients who received eDFET had fewer implantations (30.9%) than eSFET (52.5%; P = 0.004); pregnancy rates were similar (eDFET: 35.3%, eSFET: 42.5%). Patients with the eSFET had one monozygotic twin (5.9%), and 22.2% of eDFET patients had multiple pregnancies. Patients with a good prognosis who failed to conceive in the first fresh eSET did not have an advantage when receiving eDFET in the second cycle, as pregnancy rates were similar; 22.2% of patients in the eDFET group had multiple pregnancies.